Beta-Sitosterol (purity>98%) (Synonyms: β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%))

Name: Beta-Sitosterol (purity>98%)
Brand: MedChemExpress
SKU: HY-N0171A
Rating: 5.0 (7 reviews)

Cat. No.: HY-N0171A Purity: 99.47%: FAQs
Data Sheet SDS COA Handling Instructions

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Beta-Sitosterol (purity>98%) is a plant sterol. Beta-Sitosterol (purity>98%) interfere with multiple cell signaling pathways, including cell cycle, apoptosis, proliferation, survival, invasion, angiogenesis, metastasis and inflammation.

For research use only. We do not sell to patients.

Beta-Sitosterol (purity>98%) Chemical Structure

CAS No. : 83-46-5

Size	Price	Stock	Quantity
5 mg	USD 41	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
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Based on 7 publication(s) in Google Scholar

Other Forms of Beta-Sitosterol (purity>98%):

Beta-Sitosterol (purity>80%) In-stock
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7 Publications Citing Use of MCE Beta-Sitosterol (purity>98%)

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
1A9	ED₅₀	10.6 μg/mL Compound: 17	Cytotoxicity against human 1A9 cells after 6 days by SRB assay Cytotoxicity against human 1A9 cells after 6 days by SRB assay	[PMID: 14640511]
1A9	ED₅₀	16.8 μg/mL Compound: 17	Cytotoxicity against human 1A9 cells after 3 days by SRB assay Cytotoxicity against human 1A9 cells after 3 days by SRB assay	[PMID: 14640511]
1A9/ptx-10	ED₅₀	20 μg/mL Compound: 17	Cytotoxicity against human 1A9/PTX10 cells after 3 days by SRB assay Cytotoxicity against human 1A9/PTX10 cells after 3 days by SRB assay	[PMID: 14640511]
1A9/ptx-10	ED₅₀	9.5 μg/mL Compound: 17	Cytotoxicity against human 1A9/PTX10 cells after 6 days by SRB assay Cytotoxicity against human 1A9/PTX10 cells after 6 days by SRB assay	[PMID: 14640511]
A2780	IC₅₀	> 10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human A2780 cells after 96 hrs by MTT assay Cytotoxicity against human A2780 cells after 96 hrs by MTT assay	[PMID: 17125236]
A549	IC₅₀	> 10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human A549 cells after 96 hrs by MTT assay Cytotoxicity against human A549 cells after 96 hrs by MTT assay	[PMID: 17125236]
A549	ED₅₀	> 20 μg/mL Compound: 17	Cytotoxicity against human A549 cells after 3 days by SRB assay Cytotoxicity against human A549 cells after 3 days by SRB assay	[PMID: 14640511]
Bel-7402	IC₅₀	> 10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay	[PMID: 17125236]
BGC-823	IC₅₀	> 10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay	[PMID: 17125236]
ECV-304	IC₅₀	472 μM Compound: Beta-sitosterol	Membrane toxicity against human ECV304 cells after 2 hrs by LDH release assay Membrane toxicity against human ECV304 cells after 2 hrs by LDH release assay	[PMID: 24084294]
ECV-304	IC₅₀	472 μM Compound: beta-sitosterol	Membranolytic activity in human ECV304 cells assessed as leakage of intracellular lactate dehydrogenase after 2 hrs by spectrophotometry Membranolytic activity in human ECV304 cells assessed as leakage of intracellular lactate dehydrogenase after 2 hrs by spectrophotometry	[PMID: 22503361]
ECV-304	IC₅₀	61 μM Compound: beta-sitosterol	Cytotoxicity against human ECV304 cells after 72 hrs by Hoechst 33258 staining based fluorescence assay Cytotoxicity against human ECV304 cells after 72 hrs by Hoechst 33258 staining based fluorescence assay	[PMID: 22503361]
ECV-304	IC₅₀	61 μM Compound: Beta-sitosterol	Cytotoxicity against human ECV304 cells after 72 hrs by MTT assay Cytotoxicity against human ECV304 cells after 72 hrs by MTT assay	[PMID: 24084294]
HCT-8	IC₅₀	> 10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay	[PMID: 17125236]
HCT-8	ED₅₀	> 20 μg/mL Compound: 17	Cytotoxicity against human HCT8 cells after 3 days by SRB assay Cytotoxicity against human HCT8 cells after 3 days by SRB assay	[PMID: 14640511]
HeLa	IC₅₀	46.22 μM Compound: 9	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19447618]
HEp-2	IC₅₀	11.4 μM Compound: 119	Antiproliferative activity against human Hep2 cells by MTT assay Antiproliferative activity against human Hep2 cells by MTT assay	[PMID: 30830783]
J774	IC₅₀	> 241.1 μM Compound: 11	Cytotoxicity against mouse J774 cells by alamar blue assay Cytotoxicity against mouse J774 cells by alamar blue assay	[PMID: 17637068]
KB	ED₅₀	> 20 μg/mL Compound: 17	Cytotoxicity against human KB cells after 3 days by SRB assay Cytotoxicity against human KB cells after 3 days by SRB assay	[PMID: 14640511]
MCF7	ED₅₀	> 20 μg/mL Compound: 17	Cytotoxicity against human MCF7 cells after 3 days by SRB assay Cytotoxicity against human MCF7 cells after 3 days by SRB assay	[PMID: 14640511]
MCF7	IC₅₀	42.1 μM Compound: 9	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 19447618]
PC-3	ED₅₀	> 20 μg/mL Compound: 17	Cytotoxicity against human PC3 cells after 3 days by SRB assay Cytotoxicity against human PC3 cells after 3 days by SRB assay	[PMID: 14640511]
SK-MEL-1	IC₅₀	> 50 μM Compound: 9	Cytotoxicity against human SK-MEL-1 cells by MTT assay Cytotoxicity against human SK-MEL-1 cells by MTT assay	[PMID: 19447618]
U-87MG ATCC	ED₅₀	> 20 μg/mL Compound: 17	Cytotoxicity against human U87MG cells after 3 days by SRB assay Cytotoxicity against human U87MG cells after 3 days by SRB assay	[PMID: 14640511]

In Vitro

Beta-Sitosterol is one of the most abundant dietary phytosterols. Beta-Sitosterol shows anticancer properties against breast cancer, prostate cancer, colon cancer, lung cancer, stomach cancer, ovarian cancer, and leukemia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

414.71

Formula

C₂₉H₅₀O

CAS No.

83-46-5

Appearance

Solid

Color

White to off-white

SMILES

CC[C@@H](C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

Ethanol : 5 mg/mL (12.06 mM; ultrasonic and warming and heat to 60°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4113 mL	12.0566 mL	24.1132 mL
5 mM	0.4823 mL	2.4113 mL	4.8226 mL
10 mM	0.2411 mL	1.2057 mL	2.4113 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.41 mM); Suspended solution

This protocol yields a suspended solution of ≥ 1 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 1 mg/mL (2.41 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Corn Oil
Solubility: 10 mg/mL (24.11 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 5 mg/mL (12.06 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) RP-HPLC (219 KB)

Handling Instructions (2659 KB)

References

[1]. Bin Sayeed MS, et al. Beta-Sitosterol: A Promising but Orphan Nutraceutical to Fight Against Cancer. Nutr Cancer. 2015;67(8):1214-20. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.4113 mL	12.0566 mL	24.1132 mL	60.2831 mL
	5 mM	0.4823 mL	2.4113 mL	4.8226 mL	12.0566 mL
	10 mM	0.2411 mL	1.2057 mL	2.4113 mL	6.0283 mL

Beta-Sitosterol (purity>98%) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Beta-Sitosterol (purity>98%) (Synonyms: β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%))

Customer Review

Other Forms of Beta-Sitosterol (purity>98%):

Beta-Sitosterol (purity>98%) Related Antibodies

7 Publications Citing Use of MCE Beta-Sitosterol (purity>98%)

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Beta-Sitosterol (purity>98%) Related Antibodies

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Complete Stock Solution Preparation Table

Beta-Sitosterol (purity>98%) Related Classifications

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