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C3TD879 is an inhibitor of citron kinase (CITK), an AGC family serine/threonine kinase that regulates cytokinesis. C3TD879 inhibits CITK catalytic activity with an IC50 of 12 nM. C3TD879 does not induce CITK knockdown effects on cell proliferation, cell cycle progression, or cytokinesis, but rather reduces the activity by directly binding full-length human CITK (NanoBRET Kd< 10 nM).

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C3TD879 Chemical Structure

C3TD879 Chemical Structure

CAS No. : 2850259-33-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 616 In-stock
Solution
10 mM * 1 mL in DMSO USD 616 In-stock
Solid
1 mg USD 224 In-stock
5 mg USD 560 In-stock
10 mg USD 900 In-stock
25 mg USD 1800 In-stock
50 mg USD 2900 In-stock
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Based on 1 publication(s) in Google Scholar

C3TD879 Related Antibodies

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Description

C3TD879 is an inhibitor of citron kinase (CITK), an AGC family serine/threonine kinase that regulates cytokinesis. C3TD879 inhibits CITK catalytic activity with an IC50 of 12 nM. C3TD879 does not induce CITK knockdown effects on cell proliferation, cell cycle progression, or cytokinesis, but rather reduces the activity by directly binding full-length human CITK (NanoBRET Kd< 10 nM)[1].

IC50 & Target

IC50: 12 nM (Citron kinase, CITK)[1]
Molecular Weight

364.48

Formula

C22H28N4O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=CC(NC([C@H](N)CC(C)(C)C)=O)=CC=C1C2=C3C(NC(C)=C3)=NC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (274.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7436 mL 13.7182 mL 27.4363 mL
5 mM 0.5487 mL 2.7436 mL 5.4873 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7436 mL 13.7182 mL 27.4363 mL 68.5909 mL
5 mM 0.5487 mL 2.7436 mL 5.4873 mL 13.7182 mL
10 mM 0.2744 mL 1.3718 mL 2.7436 mL 6.8591 mL
15 mM 0.1829 mL 0.9145 mL 1.8291 mL 4.5727 mL
20 mM 0.1372 mL 0.6859 mL 1.3718 mL 3.4295 mL
25 mM 0.1097 mL 0.5487 mL 1.0975 mL 2.7436 mL
30 mM 0.0915 mL 0.4573 mL 0.9145 mL 2.2864 mL
40 mM 0.0686 mL 0.3430 mL 0.6859 mL 1.7148 mL
50 mM 0.0549 mL 0.2744 mL 0.5487 mL 1.3718 mL
60 mM 0.0457 mL 0.2286 mL 0.4573 mL 1.1432 mL
80 mM 0.0343 mL 0.1715 mL 0.3430 mL 0.8574 mL
100 mM 0.0274 mL 0.1372 mL 0.2744 mL 0.6859 mL
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C3TD879 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C3TD8792850259-33-3OthersCitron kinaseCITKType I kinaseInhibitorinhibitorinhibit

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