2. Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK JAK/STAT Signaling Epigenetics Stem Cell/Wnt Apoptosis
  3. Endogenous Metabolite JAK Apoptosis
  4. Crocin

Crocin (Crocin I) is an orally active natural product that can be isolated from the stigma of Crocus sativus. Crocin inhibits tumor cell proliferation and promotes apoptosis through JAK pathway. Crocin has anti-inflammatory, antioxidant and antitumor activities .

For research use only. We do not sell to patients.

Crocin Chemical Structure

Crocin Chemical Structure

CAS No. : 42553-65-1

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Crocin:

Crocin Related Antibodies

Top Publications Citing Use of Products

    Crocin purchased from MedChemExpress. Usage Cited in: Biomolecules. 2022, 12(12), 1813.

    Crocin (5, 10, 50,100 μM) significantly increases the level of ERK1/2 phosphorylation in GHSR1a-mCherry HEK293T cells.

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    JAK1 JAK2 JAK3 Tyk2 JAK

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Crocin (Crocin I) is an orally active natural product that can be isolated from the stigma of Crocus sativus. Crocin inhibits tumor cell proliferation and promotes apoptosis through JAK pathway. Crocin has anti-inflammatory, antioxidant and antitumor activities [1][2].

    Cellular Effect
    Cell Line Type Value Description References
    RAW264.7 IC50
    > 100 μM
    Compound: 16, crocin
    Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method
    Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method
    		[PMID: 23305920]
    In Vitro

    Crocin (150, 200 μM, 24 h) plays an antitumor role in colon cancer cells by inhibiting the JAK pathway[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Crocin Related Antibodies

    Cell Viability Assay[1]

    Cell Line: HCT116
    Concentration: 0, 50, 100, 150, 200, 250, 300, 350, 400, and 450 μM
    Incubation Time: 24 h
    Result: Weakened the cell vitality in a concentration-dependent manner.

    Apoptosis Analysis[1]

    Cell Line: HCT116
    Concentration: 150, 200 μM
    Incubation Time: 24 h
    Result: Facilitated apoptosis of HCT-116 cells in a concentration-dependent manner.
    Abated the colony number and downregulated the positive expression of Ki-67.

    Western Blot Analysis[1]

    Cell Line: HCT116
    Concentration: 150, 200 μM
    Incubation Time: 24 h
    Result: Decreased the levels of p-JAK2, p-STAT3, and p-ERK, as well as the ratios of p-JAK2/JAK2, p-STAT3/STAT3, and pERK/ERK.
    In Vivo

    Crocin (20 mg/kg, orally, for 5 consecutive weeks) shows significant antioxidant, anti-inflammatory and antifibrotic effects on Bleomycin (HY-108345) -induced pulmonary fibrosis in rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Bleomycin-induced pulmonary fibrosis in rat[2]
    Dosage: 20 mg/kg
    Administration: p.o.
    Result: Reduced the expression of TLR4 and IL-10.
    Reduced the expression of TNF-α and TGF-β1.
    Clinical Trial
    Molecular Weight

    976.96

    Formula

    C44H64O24
    CAS No.
    Appearance

    Solid

    Color

    Pink to red

    SMILES

    O[C@@H]([C@H](O)[C@H]1O)[C@H](O[C@H]1OC(/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C(O[C@@H]([C@@H]([C@@H](O)[C@@H]2O)O)O[C@@H]2CO[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=O)=O)CO[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (127.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.0236 mL 5.1179 mL 10.2358 mL
    5 mM 0.2047 mL 1.0236 mL 2.0472 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (2.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (2.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.68%

    SDS (251 KB)

    COA (249 KB) HNMR (346 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.0236 mL 5.1179 mL 10.2358 mL 25.5896 mL
    5 mM 0.2047 mL 1.0236 mL 2.0472 mL 5.1179 mL
    10 mM 0.1024 mL 0.5118 mL 1.0236 mL 2.5590 mL
    15 mM 0.0682 mL 0.3412 mL 0.6824 mL 1.7060 mL
    20 mM 0.0512 mL 0.2559 mL 0.5118 mL 1.2795 mL
    25 mM 0.0409 mL 0.2047 mL 0.4094 mL 1.0236 mL
    30 mM 0.0341 mL 0.1706 mL 0.3412 mL 0.8530 mL
    40 mM 0.0256 mL 0.1279 mL 0.2559 mL 0.6397 mL
    50 mM 0.0205 mL 0.1024 mL 0.2047 mL 0.5118 mL
    60 mM 0.0171 mL 0.0853 mL 0.1706 mL 0.4265 mL
    80 mM 0.0128 mL 0.0640 mL 0.1279 mL 0.3199 mL
    100 mM 0.0102 mL 0.0512 mL 0.1024 mL 0.2559 mL
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    Crocin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Crocin42553-65-1Crocin IEndogenous MetaboliteJAKApoptosisJanus kinaseAnti-inflammatoryAntitumorHCT116BleomycinInhibitorinhibitorinhibit

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