2. Cell Cycle/DNA Damage Epigenetics Apoptosis Metabolic Enzyme/Protease
  3. HDAC Apoptosis Drug Metabolite
  4. DL-Sulforaphane N-acetyl-L-cysteine

DL-Sulforaphane N-acetyl-L-cysteine  (Synonyms: SFN-NAC)

Cat. No.: HY-132242 Purity: ≥99.0%
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DL-Sulforaphane N-acetyl-L-cysteine (SFN-NAC) is an orally active HDAC inhibitor and metabolite of sulforaphane (HY-13755) with longer half-life and better blood-brain barrier permeability. DL-Sulforaphane N-acetyl-L-cysteine activates autophagy-mediated downregulation of α-tubulin expression through the ERK pathway and can be used in cancer research.

For research use only. We do not sell to patients.

DL-Sulforaphane N-acetyl-L-cysteine Chemical Structure

DL-Sulforaphane N-acetyl-L-cysteine Chemical Structure

CAS No. : 334829-66-2

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5 mg USD 499 In-stock
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Based on 1 publication(s) in Google Scholar

DL-Sulforaphane N-acetyl-L-cysteine Related Antibodies

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HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

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Description

DL-Sulforaphane N-acetyl-L-cysteine (SFN-NAC) is an orally active HDAC inhibitor and metabolite of sulforaphane (HY-13755) with longer half-life and better blood-brain barrier permeability. DL-Sulforaphane N-acetyl-L-cysteine activates autophagy-mediated downregulation of α-tubulin expression through the ERK pathway and can be used in cancer research[1][2].

Cellular Effect
Cell Line Type Value Description References
LoVo IC50
20.5 μM
Compound: NAC-SFN
Antiproliferative activity against human LoVo cells after 72 hrs by SRB assay
Antiproliferative activity against human LoVo cells after 72 hrs by SRB assay
[PMID: 29945793]
In Vitro

SFN-NAC (24 h) decreases cell viability, with IC50 values of 60.08 μM for HA, 35.20 μM for U87MG, 39.11 μM for U373MG, and 36.20 μM for the U87/TR cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DL-Sulforaphane N-acetyl-L-cysteine Related Antibodies

Cell Cycle Analysis[1]

Cell Line: U87MG and U373MG cells
Concentration: 0, 30 μM
Incubation Time: 24 h
Result: Induced cell-cycle arrest in the G2/M phase and triggered apoptosis at the same time.

Western Blot Analysis[1]

Cell Line: U87MG and U373MG cells
Concentration: 0, 10, 20, 30, 40, 50μM
Incubation Time: 24 h
Result: Activated ERK1/2 (Thr202/Tyr204), downregulated α-tubulin, and induced autophagy in a dose-dependent manner.

Cell Viability Assay[1]

Cell Line: HA, U87MG, U373MG and U87/TR cells
Concentration: 0, 10, 20, 30, 40, 50, 60, 70, 80, 90 μM for HA and U87MG cells or 0, 10, 20, 30, 40, 50, 60 μM for U373MG and U87/TR cells
Incubation Time: 24 h
Result: Decreased the cell viability of these cell lines in a dose-dependent manner.
In Vivo

SFN-NAC (10 μmol; 6 h; Oral gavage; single dose) significantly inhibits HDAC activity in mouse colon mucosa[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: mice[2]
Dosage: 10 μmol
Administration: Oral gavage; 6 h; single dose
Result: Significantly inhibited HDAC activity in mouse colon mucosa.
Molecular Weight

340.48

Formula

C11H20N2O4S3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CS(CCCCNC(SC[C@@H](C(O)=O)NC(C)=O)=S)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (29.37 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9370 mL 14.6852 mL 29.3703 mL
5 mM 0.5874 mL 2.9370 mL 5.8741 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.90%

SDS (251 KB)

COA (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9370 mL 14.6851 mL 29.3703 mL 73.4257 mL
5 mM 0.5874 mL 2.9370 mL 5.8741 mL 14.6851 mL
10 mM 0.2937 mL 1.4685 mL 2.9370 mL 7.3426 mL
15 mM 0.1958 mL 0.9790 mL 1.9580 mL 4.8950 mL
20 mM 0.1469 mL 0.7343 mL 1.4685 mL 3.6713 mL
25 mM 0.1175 mL 0.5874 mL 1.1748 mL 2.9370 mL
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DL-Sulforaphane N-acetyl-L-cysteine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DL-Sulforaphane N-acetyl-L-cysteine334829-66-2SFN-NACHDACApoptosisDrug MetaboliteHistone deacetylasesGliomaAutophagySulforaphaneα-tubulinEpigeneticsEpigenomicsChromatin remodelingAcetylated histonesCancer preventionDietButyrateOrganosulfur compoundsGarlicCruciferous vegetablesInhibitorinhibitorinhibit

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