2. Cell Cycle/DNA Damage Epigenetics Apoptosis
  3. HDAC Apoptosis Caspase
  4. Elevenostat

Elevenostat  (Synonyms: JB3-22)

Cat. No.: HY-145757 Purity: 95.85%
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Elevenostat (JB3-22) is a selective HDAC11 inhibitor with an IC50 of 0.235 µM. Elevenostat can induce apoptosis of multiple myeloma cells and has anti-tumor effect. In addition, Elevenostat inhibits the maturation of mouse oocytes.

For research use only. We do not sell to patients.

Elevenostat Chemical Structure

Elevenostat Chemical Structure

CAS No. : 1454902-97-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 80 In-stock
25 mg USD 150 In-stock
50 mg USD 250 In-stock
100 mg USD 450 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Elevenostat Related Antibodies

Top Publications Citing Use of Products

View All HDAC Isoform Specific Products:

View All Isoforms
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

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Description

Elevenostat (JB3-22) is a selective HDAC11 inhibitor with an IC50 of 0.235 µM. Elevenostat can induce apoptosis of multiple myeloma cells and has anti-tumor effect. In addition, Elevenostat inhibits the maturation of mouse oocytes[1][2].

IC50 & Target[1]

HDAC11

0.235 μM (IC50)

Caspase-3

 

Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
139 nM
Compound: 1
Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate
Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate
[PMID: 23009203]
Sf9 IC50
265 nM
Compound: 1
Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate
Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate
[PMID: 23009203]
In Vitro

Elevenostat (10 μM; 4-16 h) inhibits the maturation of mouse oocytes, leading to spindle disintegration and chromosome dislocation[2].
Elevenostat (750 nM; 48 h) induces apoptosis in MM1.S cells, with about 31.4% of cells expressing activated caspase-3[3].
Elevenostat (10-8-10-4 M; 72 h) shows severe cytotoxicity to human multiple myeloma cells with an IC50 ranging from 0.803 to 3.410 μM[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Elevenostat Related Antibodies
In Vivo

Elevenostat (1-10 mg/kg; intraperitoneal injection; 0-4 weeks) has an anti-tumor effect in a mouse model of multiple myeloma[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5TGM1/C57BL/KaLwRij mouse multiple myeloma model[3]
Dosage: 1 mg/kg and 10 mg/kg
Administration: Intraperitoneal injection (i.p.); once daily for the first 10 days and every alternate day for the rest of the treatment period until mice reached disease endpoint.
Result: Effectively inhibited tumor growth.
Decrease serum IgG2b level.
Significantly prolonged the survival period of tumor-bearing mice.
Was well-tolerated at these doses and dosing frequencies, supported by lack of changes in body weight.
Molecular Weight

315.32

Formula

C16H17N3O4
CAS No.
Appearance

Solid

Color

Off-white to pink

SMILES

O=C(NO)C1=CC=C(CNC(NC2=CC=CC=C2OC)=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (792.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1714 mL 15.8569 mL 31.7138 mL
5 mM 0.6343 mL 3.1714 mL 6.3428 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.60 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (6.60 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 95.85%

SDS (251 KB)

COA (246 KB) HNMR (270 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1714 mL 15.8569 mL 31.7138 mL 79.2845 mL
5 mM 0.6343 mL 3.1714 mL 6.3428 mL 15.8569 mL
10 mM 0.3171 mL 1.5857 mL 3.1714 mL 7.9285 mL
15 mM 0.2114 mL 1.0571 mL 2.1143 mL 5.2856 mL
20 mM 0.1586 mL 0.7928 mL 1.5857 mL 3.9642 mL
25 mM 0.1269 mL 0.6343 mL 1.2686 mL 3.1714 mL
30 mM 0.1057 mL 0.5286 mL 1.0571 mL 2.6428 mL
40 mM 0.0793 mL 0.3964 mL 0.7928 mL 1.9821 mL
50 mM 0.0634 mL 0.3171 mL 0.6343 mL 1.5857 mL
60 mM 0.0529 mL 0.2643 mL 0.5286 mL 1.3214 mL
80 mM 0.0396 mL 0.1982 mL 0.3964 mL 0.9911 mL
100 mM 0.0317 mL 0.1586 mL 0.3171 mL 0.7928 mL
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Elevenostat Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Elevenostat1454902-97-6JB3-22HDACApoptosisCaspaseHistone deacetylasesmultiple myelomaHDAC11oocytesInhibitorinhibitorinhibit

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