2. Metabolic Enzyme/Protease MAPK/ERK Pathway PI3K/Akt/mTOR NF-κB
  3. Endogenous Metabolite p38 MAPK Akt Keap1-Nrf2 NF-κB
  4. Ergothioneine

Ergothioneine  (Synonyms: L-(+)-Ergothioneine)

Cat. No.: HY-N1914 Purity: 99.99%
SDS COA Handling Instructions

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Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity.

For research use only. We do not sell to patients.

Ergothioneine Chemical Structure

Ergothioneine Chemical Structure

CAS No. : 497-30-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
5 mg USD 61 In-stock
10 mg USD 95 In-stock
25 mg USD 190 In-stock
50 mg USD 365 In-stock
100 mg USD 511 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Ergothioneine:

Ergothioneine Related Antibodies

Top Publications Citing Use of Products

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK p38α p38β MAPK13 p38δ p38γ

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity[1][2].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
HEK293 EC50
40 μM
Compound: 5351619
Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4
Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4
10.5281/zenodo.7360363
In Vitro

Ergothioneine (0.25, 1 mM, 23 h) can regulate PC12 DNA damage, MAPKs activation and cell death induced by hydrogen peroxide[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ergothioneine Related Antibodies

Cell Viability Assay [1]

Cell Line: PC12
Concentration: 0.25, 1 mM
Incubation Time: 23 h
Result: Increased cell viability by approximately 13% and 34%, respectively.

Western Blot Analysis [1]

Cell Line: PC12
Concentration: 0.25, 1 mM
Incubation Time: 23 h
Result: Counteracted the p38 phosphorylation induced by 1 h incubation with H2O2.
In Vivo

Ergothioneine (70 mg/kg orally, For 14 consecutive days) mediates the improvement of cisplatin-induced nephrotoxicity in rats by regulating Nrf2, p53 and NF-κB signaling and inhibiting γ-glutamyltranspeptidase[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Cisplatin-treated rats[1]
Dosage: 70 mg/kg
Administration: p.o. For 14 consecutive days
Result: Decreased serum creatinine and BUN.
Increased GFR at two-fold.
Suppressed cisplatin-induced oxidative stress and GCT.
Up-regulated the levels of Nrf2, NQO1, and HO-1.
Suppresses NF-κB signaling and the pro-inflammatory cytokines.
Clinical Trial
Molecular Weight

229.30

Formula

C9H15N3O2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

S=C1NC(C[C@@H](C([O-])=O)[N+](C)(C)C)=CN1
Structure Classification
Initial Source

many bacteria and most fungi
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (545.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3611 mL 21.8055 mL 43.6110 mL
5 mM 0.8722 mL 4.3611 mL 8.7222 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (436.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.99%

SDS (393 KB)

COA (249 KB) HNMR (247 KB) LCMS (265 KB) OR (203 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.3611 mL 21.8055 mL 43.6110 mL 109.0275 mL
5 mM 0.8722 mL 4.3611 mL 8.7222 mL 21.8055 mL
10 mM 0.4361 mL 2.1805 mL 4.3611 mL 10.9027 mL
15 mM 0.2907 mL 1.4537 mL 2.9074 mL 7.2685 mL
20 mM 0.2181 mL 1.0903 mL 2.1805 mL 5.4514 mL
25 mM 0.1744 mL 0.8722 mL 1.7444 mL 4.3611 mL
30 mM 0.1454 mL 0.7268 mL 1.4537 mL 3.6342 mL
40 mM 0.1090 mL 0.5451 mL 1.0903 mL 2.7257 mL
50 mM 0.0872 mL 0.4361 mL 0.8722 mL 2.1805 mL
60 mM 0.0727 mL 0.3634 mL 0.7268 mL 1.8171 mL
80 mM 0.0545 mL 0.2726 mL 0.5451 mL 1.3628 mL
100 mM 0.0436 mL 0.2181 mL 0.4361 mL 1.0903 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ergothioneine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ergothioneine497-30-3L-(+)-ErgothioneineEndogenous Metabolitep38 MAPKAktKeap1-Nrf2NF-κBPKBProtein kinase BNuclear factor-κBNuclear factor-kappaBMAPKAntioxidantPC12CisplatinDNA fragmentationInhibitorinhibitorinhibit

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