2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Estrogen Receptor/ERR Endogenous Metabolite
  4. Ethinylestradiol

Ethinylestradiol  (Synonyms: Ethynyl estradiol; 17α-Ethynylestradiol)

Cat. No.: HY-B0216 Purity: 99.91%
COA Handling Instructions

Molarity Calculator

Ethinylestradiol is an orally active steroidal estrogen. Ethinylestradiol is widely used in research on menopausal symptoms, gynecological conditions, and certain hormone-sensitive cancers.

For research use only. We do not sell to patients.

Ethinylestradiol Chemical Structure

Ethinylestradiol Chemical Structure

CAS No. : 57-63-6

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 44 In-stock
Solution
10 mM * 1 mL in DMSO USD 44 In-stock
Solid
500 mg USD 30 In-stock
1 g USD 58 In-stock
5 g USD 184 In-stock
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Ethinylestradiol:

Ethinylestradiol Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Ethinylestradiol

View All Estrogen Receptor/ERR Isoform Specific Products:

View All Isoforms
Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ethinylestradiol is an orally active steroidal estrogen. Ethinylestradiol is widely used in research on menopausal symptoms, gynecological conditions, and certain hormone-sensitive cancers[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Ethinylestradiol (0.01-10 nM, 24-48 h) increases cGMP formation in RFL6 cells[2].
Ethinylestradiol (0.01-10 nM, 6-48 h) reduces superoxide anion production in BAEC cells in dose- and time-dependent manner[2].
Ethinylestradiol (1-100 nM, 24 h) decreases mRNA (XPC and XPA) abundance and NER capacity in zebrfish liver cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ethinylestradiol Related Antibodies
In Vivo

Ethinylestradiol (5 mg/kg, s.c., 5 days) increases synthesis and expression of low density lipoprotein-receptor in the liver of female Sprague-Dawley rats at pharmacological doses[4].
Ethinylestradiol (0-50 μg/kg, i.g., daily, 21 days) can have adverse effects on the reproductive development of nulliparous female Wistar rats[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Sprague-Dawley rats[4]
Dosage: 5 mg/kg
Administration: Subcutaneous injection (s.c.), 5 days
Result: Decreases plasma cholesterol levels and cholesterol content.
Increased low density lipoprotein tissue spaces and clearance rates in the liver.
Enhanced the hepatic expression of low density lipoprotein-receptor protein and mRNA.
Increased cholesterol synthesis in several extrahepatic tissues, such as adrenals, ovaries, small bowel, and spleen.
Animal Model: Nulliparous female Wistar rats[5]
Dosage: 0-50 μg/kg
Administration: i.g., daily, 21 days
Result: Increased number of nipples and reduced ovary weight in female offspring.
Induced malformations of female genitalia.
Deepened the width of urethral slits in adult rats.
Increased the expression of estrogen-regulated gene in ventral prostate of prepubertal male offspring in a dose-dependent manner.
Decreased ventral prostate weight at 15μg/kg in prepubertal male offspring.
Clinical Trial
Molecular Weight

296.40

Formula

C20H24O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C2C(CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4(C#C)O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (337.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3738 mL 16.8691 mL 33.7382 mL
5 mM 0.6748 mL 3.3738 mL 6.7476 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.91%

COA (250 KB) HNMR (246 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3738 mL 16.8691 mL 33.7382 mL 84.3455 mL
5 mM 0.6748 mL 3.3738 mL 6.7476 mL 16.8691 mL
10 mM 0.3374 mL 1.6869 mL 3.3738 mL 8.4345 mL
15 mM 0.2249 mL 1.1246 mL 2.2492 mL 5.6230 mL
20 mM 0.1687 mL 0.8435 mL 1.6869 mL 4.2173 mL
25 mM 0.1350 mL 0.6748 mL 1.3495 mL 3.3738 mL
30 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8115 mL
40 mM 0.0843 mL 0.4217 mL 0.8435 mL 2.1086 mL
50 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6869 mL
60 mM 0.0562 mL 0.2812 mL 0.5623 mL 1.4058 mL
80 mM 0.0422 mL 0.2109 mL 0.4217 mL 1.0543 mL
100 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ethinylestradiol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethinylestradiol57-63-6Ethynyl estradiol 17α-EthynylestradiolEstrogen Receptor/ERREndogenous Metabolitesteroidal estrogenorally activeRFL6 cellsBAEC cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ethinylestradiol
Cat. No.:
HY-B0216
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.