Ezabenlimab  (Synonyms: BI-754091)

Ezabenlimab (BI-754091) is an anti-PD-1 mAb with binding constant K_d value of 6 nM (CHO cells). Ezabenlimab blocks the interaction of PD-1 with PD-L1 and PD-L2. Ezabenlimab increases interferon-γ secretion in T cells, and inhibits tumor growth in vivo^[1].

Human IgG4 kappa

Human IgG4 (S228P) kappa, Isotype Control

Humanized

PD-1/PD-L1, PD-1/PD-L2, IFN-γ^[1]

Ezabenlimab inhibits the interaction of PD-1 to PD-L1 and PD-L2 in CHO cells with IC₉₀s of 4.14 nM and 3.98 nM, respectively; and inhibits the interaction of PD-1 to PD-L1 and PD-L2 in cynomolgus with IC₉₀s of 5.61 nM and 7.54 nM, respectively^[1].
Ezabenlimab increases IFN-γ secretion in primary human antigen-experienced CD3-positive T cells, with an EC₅₀ value of 0.9 nM^[1].
Ezabenlimab (200 nM) has synergistic effect with BI 754111, resulting in further increase of IFN-γ secretion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ezabenlimab (0.3-30 mg/kg; i.p.; single dose or twice per week or every 3 weeks) inhibits tumor progress and shows comparable anti-tumor activity in hPD-1 knock-in mouse model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2249882-54-8

Liquid

Colorless to light yellow

[Ezabenlimab]

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

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  Human IgG4 kappa

• 
  Immobilized Human PD-1 Protein (ECD, His Tag) can bind Ezabenlimab. The EC₅₀ for this effect is 3.34 ng/mL.

• [1]. Zettl M, et al. Combination of two novel blocking antibodies, anti-PD-1 antibody ezabenlimab (BI 754091) and anti-LAG-3 antibody BI 754111, leads to increased immune cell responses. Oncoimmunology. 2022 Jun 16;11(1):2080328.  [Content Brief]