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CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution.

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CHAPS Chemical Structure

CHAPS Chemical Structure

CAS No. : 75621-03-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
500 mg USD 50 In-stock
1 g USD 60 In-stock
5 g USD 119 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of CHAPS:

CHAPS Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CHAPS, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution[1][2].

In Vitro

CHAPS (0.5 %) is able to stabilize complexes between DNA and DNA-binding factors such as AP-1, SPI, GATA-1 and α-regulated factor ISGF3, and retains their biochemical activity[2].
CHAPS can prevent dissociation of mononucleosomes diluted to sub-nanomolar concentrations[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CHAPS Related Antibodies
Molecular Weight

614.88

Formula

C32H58N2O7S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1CC[C@]2(C)[C@@]3([H])C[C@H](O)[C@]4(C)[C@@H]([C@H](C)CCC(NCCC[N+](C)(C)CCCS([O-])(=O)=O)=O)CC[C@@]4([H])[C@]3([H])[C@H](O)C[C@@]([H])2C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 116.67 mg/mL (189.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 83.33 mg/mL (135.52 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6263 mL 8.1317 mL 16.2633 mL
5 mM 0.3253 mL 1.6263 mL 3.2527 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 10 mg/mL (16.26 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.87%

COA (247 KB) HNMR (283 KB) RP-HPLC (215 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.6263 mL 8.1317 mL 16.2633 mL 40.6583 mL
5 mM 0.3253 mL 1.6263 mL 3.2527 mL 8.1317 mL
10 mM 0.1626 mL 0.8132 mL 1.6263 mL 4.0658 mL
15 mM 0.1084 mL 0.5421 mL 1.0842 mL 2.7106 mL
20 mM 0.0813 mL 0.4066 mL 0.8132 mL 2.0329 mL
25 mM 0.0651 mL 0.3253 mL 0.6505 mL 1.6263 mL
30 mM 0.0542 mL 0.2711 mL 0.5421 mL 1.3553 mL
40 mM 0.0407 mL 0.2033 mL 0.4066 mL 1.0165 mL
50 mM 0.0325 mL 0.1626 mL 0.3253 mL 0.8132 mL
60 mM 0.0271 mL 0.1355 mL 0.2711 mL 0.6776 mL
80 mM 0.0203 mL 0.1016 mL 0.2033 mL 0.5082 mL
100 mM 0.0163 mL 0.0813 mL 0.1626 mL 0.4066 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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CHAPS Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CHAPS75621-03-3OtherszwitterionicdetergentsurfactantproteincomplexesstabilizedInhibitorinhibitorinhibit

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