GB-110 hydrochloride

Name: GB-110 hydrochloride
Brand: MedChemExpress
SKU: HY-120528A
Rating: 4.5 (1 reviews)

Cat. No.: HY-120528A Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

GB-110 hydrochloride is a potent, orally active, and nonpeptidic protease activated receptor 2 (PAR2) agonist. GB-110 hydrochloride selectively induces PAR2-mediated intracellular Ca²⁺ release in HT29 cells with an EC₅₀ of 0.28 μM.

For research use only. We do not sell to patients.

GB-110 hydrochloride Chemical Structure

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 497	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 497	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 990	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of GB-110 hydrochloride:

GB-110 Get quote

1 Publications Citing Use of MCE GB-110 hydrochloride

•Cell Mol Gastroenterol Hepatol. 2022 Jul 14;S2352-345X(22)00157-6. [Abstract]

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Protease Activated Receptor (PAR) Isoform Specific Products:

View All Isoforms

PAR1 PAR2 PAR4

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PAR2

.28 μM (EC₅₀)

In Vitro

In an intracellular Ca²⁺ (iCa²⁺) mobilization assay using HT29 colon cancer cells, GB110 (EC₅₀ 240±20 nM; pEC₅₀ 6.7±0.05) is equipotent with the peptide agonist 2f-LIGRLO-NH2 (EC₅₀ 210±30 nM; pEC₅₀ 6.6±0.05), 10-fold more potent than SLIGRL-NH2, but ~35-fold less potent than trypsin (EC₅₀ 6±0.5 nM; pEC₅₀ 8.2±0.8)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

645.23

Formula

C₃₃H₄₉ClN₆O₅

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=NO1)N[C@@H](CC2CCCCC2)C(N[C@@H]([C@@H](C)CC)C(NCC3=CC=CC(C(N4CCC(CN)CC4)=O)=C3)=O)=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (193.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (38.75 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5498 mL	7.7492 mL	15.4983 mL
5 mM	0.3100 mL	1.5498 mL	3.0997 mL
10 mM	0.1550 mL	0.7749 mL	1.5498 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.33 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.33 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.33 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.33 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (23.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (246 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Barry GD, et al. Novel agonists and antagonists for human protease activated receptor 2. J Med Chem. 2010 Oct 28;53(20):7428-40. [Content Brief]

[2]. Suen JY, et al. Modulating human proteinase activated receptor 2 with a novel antagonist (GB88) and agonist (GB110). Br J Pharmacol. 2012 Mar;165(5):1413-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.5498 mL	7.7492 mL	15.4983 mL	38.7459 mL
	5 mM	0.3100 mL	1.5498 mL	3.0997 mL	7.7492 mL
	10 mM	0.1550 mL	0.7749 mL	1.5498 mL	3.8746 mL
	15 mM	0.1033 mL	0.5166 mL	1.0332 mL	2.5831 mL
	20 mM	0.0775 mL	0.3875 mL	0.7749 mL	1.9373 mL
	25 mM	0.0620 mL	0.3100 mL	0.6199 mL	1.5498 mL
	30 mM	0.0517 mL	0.2583 mL	0.5166 mL	1.2915 mL
DMSO	40 mM	0.0387 mL	0.1937 mL	0.3875 mL	0.9686 mL
	50 mM	0.0310 mL	0.1550 mL	0.3100 mL	0.7749 mL
	60 mM	0.0258 mL	0.1292 mL	0.2583 mL	0.6458 mL
	80 mM	0.0194 mL	0.0969 mL	0.1937 mL	0.4843 mL
	100 mM	0.0155 mL	0.0775 mL	0.1550 mL	0.3875 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.