2. Apoptosis MAPK/ERK Pathway PI3K/Akt/mTOR NF-κB Stem Cell/Wnt Neuronal Signaling Epigenetics
  3. Apoptosis p38 MAPK Akt NF-κB Notch Bcl-2 Family MDM-2/p53 Epigenetic Reader Domain
  4. Hexamethylene bisacetamide

Hexamethylene bisacetamide  (Synonyms: HMBA)

Cat. No.: HY-124284 Purity: 99.56%
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Hexamethylene bisacetamide (HMBA) is a differentiation inducer and selective bromine domain inhibitor that can differentiate across the blood-brain barrier. Hexamethylene bisacetamide can induce tumor cell differentiation and inhibit cell proliferation, showing antitumor activity. Hexamethylene bisacetamide induces apoptosis by Notch1, Bcl-2 and p53 signaling pathways. In addition, Hexamethylene bisacetamide improves the obesity phenotype of mice.

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Hexamethylene bisacetamide Chemical Structure

Hexamethylene bisacetamide Chemical Structure

CAS No. : 3073-59-4

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Based on 1 publication(s) in Google Scholar

Hexamethylene bisacetamide Related Antibodies

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

  • Biological Activity

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Description

Hexamethylene bisacetamide (HMBA) is a differentiation inducer and selective bromine domain inhibitor that can differentiate across the blood-brain barrier. Hexamethylene bisacetamide can induce tumor cell differentiation and inhibit cell proliferation, showing antitumor activity. Hexamethylene bisacetamide induces apoptosis by Notch1, Bcl-2 and p53 signaling pathways. In addition, Hexamethylene bisacetamide improves the obesity phenotype of mice[1][2][3][4][5].

In Vitro

Hexamethylene bisacetamide (2.5-10 mM; 15 days) significantly inhibits the proliferation of SHG-44 cells in a dose-dependent manner, but the inhibitory effect is reversible[2].
Hexamethylene bisacetamide (5-10 mM; 15 days) changes the morphology of SHG-44 cells and increases the degree of cell differentiation[2].
Hexamethylene bisacetamide (1-20 mM; 30-480 minutes) inhibits the activation of MAPK, Akt signaling pathways and NF - κB in TNFα-treated A549 cells[3].
Hexamethylene bisacetamide (5 mM; 2 h-7 days) induces apoptosis through Notch1, Bcl - 2 and p53 signaling pathways in Molt4 cells[4].
Hexamethylene bisacetamide (0.1 mM; 2 h) regulates the expression of neuropeptides through MYH9 and ACTG1 in hypothalamic cells[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hexamethylene bisacetamide Related Antibodies

Cell Viability Assay[2]

Cell Line: Human malignant glioma cell line SHG-44
Concentration: 2.5, 5, 7.5 and 10 mM
Incubation Time: 15 days
Result: Reduced cell proliferation compared with the control group.

Western Blot Analysis[3]

Cell Line: A549 cells
Concentration: 10 mM
Incubation Time: 30, 60, 120 and 240 min
Result: Inhibited the phosphorylation levels of p44/p42 MAPK and MEK1/2.
Inhibited the phosphorylation levels of Akt.

Western Blot Analysis[4]

Cell Line: Molt4 cells
Concentration: 5 mM
Incubation Time: 2 h, 4 h, 8 h, 24 h, 48 h, 5 days and 7 days
Result: Reduced the levels of Notch1.
In Vivo

Hexamethylene bisacetamide (1000 mg/kg intraperitoneal injection; 500-1000 mg/kg intravenous injection; 400 nmoles 2 μL intracerebroventricular injection; 7 days) has an improved effect in mouse obesity model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice with diet-induced obese (DIO) model[5]
Dosage: 1,000 mg/kg (i.p.);
500 and 1,000 mg/kg (i.v.);
400 nmoles 2 μL (ICV)
Administration: Intraperitoneal injection (i.p.); 7 days
Intravenous injection (i.v.); 7 days
Intracerebroventricular injection (ICV); 7 days
Result: Ameliorated obese phenotype in DIO mice, reduced weight gain and food intake and improved metabolic parameters in whatever injection way.
Did not cause sickness behaviors in DIO mice in whatever injection way.
Regulate hypothalamic neuropeptide expression in whatever injection way.
Molecular Weight

200.28

Formula

C10H20N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(NCCCCCCNC(C)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (499.30 mM; Need ultrasonic)

DMSO : 25 mg/mL (124.83 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9930 mL 24.9650 mL 49.9301 mL
5 mM 0.9986 mL 4.9930 mL 9.9860 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.56%

SDS (393 KB)

COA (241 KB) HNMR (234 KB) LCMS (85 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.9930 mL 24.9650 mL 49.9301 mL 124.8252 mL
5 mM 0.9986 mL 4.9930 mL 9.9860 mL 24.9650 mL
10 mM 0.4993 mL 2.4965 mL 4.9930 mL 12.4825 mL
15 mM 0.3329 mL 1.6643 mL 3.3287 mL 8.3217 mL
20 mM 0.2497 mL 1.2483 mL 2.4965 mL 6.2413 mL
25 mM 0.1997 mL 0.9986 mL 1.9972 mL 4.9930 mL
30 mM 0.1664 mL 0.8322 mL 1.6643 mL 4.1608 mL
40 mM 0.1248 mL 0.6241 mL 1.2483 mL 3.1206 mL
50 mM 0.0999 mL 0.4993 mL 0.9986 mL 2.4965 mL
60 mM 0.0832 mL 0.4161 mL 0.8322 mL 2.0804 mL
80 mM 0.0624 mL 0.3121 mL 0.6241 mL 1.5603 mL
100 mM 0.0499 mL 0.2497 mL 0.4993 mL 1.2483 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Hexamethylene bisacetamide Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hexamethylene bisacetamide3073-59-4HMBAApoptosisp38 MAPKAktNF-κBNotchBcl-2 FamilyMDM-2/p53Epigenetic Reader DomainPKBProtein kinase BNuclear factor-κBNuclear factor-kappaBDifferentiation inducerSelective bromine domain inhibitorDIO modelSHG-44 cellsA549 cellsMolt4 cellsInhibitorinhibitorinhibit

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