HIF-1α-IN-2 hydrochloride

Name: HIF-1α-IN-2 hydrochloride
Brand: MedChemExpress
SKU: HY-115903A
Rating: 4.5 (1 reviews)

Cat. No.: HY-115903A Purity: 99.16%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

HIF-1α-IN-2 hydrochloride is an effective HIF-1α inhibitor with anticancer potencies (IC₅₀s of 28 nM and 15 nM in MDA-MB-231 and MiaPaCa-2 cells, respectively). HIF-1α-IN-2 hydrochloride suppresses HIF-1α expression by blocking transcription and protein translation.

For research use only. We do not sell to patients.

HIF-1α-IN-2 hydrochloride Chemical Structure

Size	Price	Stock	Quantity
5 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of HIF-1α-IN-2 hydrochloride:

HIF-1α-IN-2 Get quote

1 Publications Citing Use of MCE HIF-1α-IN-2 hydrochloride

•Biology (Basel). 2024 Jul 31;13(8):577. [Abstract]

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HIF-1α HIF

Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

397.92

Formula

C₂₁H₂₀ClN₃OS

Appearance

Solid

Color

Off-white to light yellow

SMILES

CC1=NC(C2=CC(NC3=CC(C)=NC4=CC(OC)=CC=C34)=CC=C2)=CS1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (251.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5131 mL	12.5653 mL	25.1307 mL
5 mM	0.5026 mL	2.5131 mL	5.0261 mL
10 mM	0.2513 mL	1.2565 mL	2.5131 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.16%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (252 KB) HNMR (221 KB) RP-HPLC (244 KB) MS (78 KB)

Handling Instructions (2659 KB)

References

[1]. Wu YC, et al. Synthesis and evaluation of biarylquinoline derivatives as novel HIF-1α inhibitors. Bioorg Chem. 2022;121:105681. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5131 mL	12.5653 mL	25.1307 mL	62.8267 mL
	5 mM	0.5026 mL	2.5131 mL	5.0261 mL	12.5653 mL
	10 mM	0.2513 mL	1.2565 mL	2.5131 mL	6.2827 mL
	15 mM	0.1675 mL	0.8377 mL	1.6754 mL	4.1884 mL
	20 mM	0.1257 mL	0.6283 mL	1.2565 mL	3.1413 mL
	25 mM	0.1005 mL	0.5026 mL	1.0052 mL	2.5131 mL
	30 mM	0.0838 mL	0.4188 mL	0.8377 mL	2.0942 mL
	40 mM	0.0628 mL	0.3141 mL	0.6283 mL	1.5707 mL
	50 mM	0.0503 mL	0.2513 mL	0.5026 mL	1.2565 mL
	60 mM	0.0419 mL	0.2094 mL	0.4188 mL	1.0471 mL
	80 mM	0.0314 mL	0.1571 mL	0.3141 mL	0.7853 mL
	100 mM	0.0251 mL	0.1257 mL	0.2513 mL	0.6283 mL