2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. HIF-IN-1

HIF-IN-1 (Compound 3c) is a hypoxia-inducible factor (HIF)-1 inhibitor. HIF-IN-1 suppresses HIF-1α protein accumulation without affecting the levels of HIF-1α mRNA. HIF-IN-1 shows no obvious cytotoxicity.

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HIF-IN-1 Chemical Structure

HIF-IN-1 Chemical Structure

CAS No. : 2393928-76-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

HIF-IN-1 Related Antibodies

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HIF-1α HIF

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Description

HIF-IN-1 (Compound 3c) is a hypoxia-inducible factor (HIF)-1 inhibitor. HIF-IN-1 suppresses HIF-1α protein accumulation without affecting the levels of HIF-1α mRNA. HIF-IN-1 shows no obvious cytotoxicity[1].

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
> 10 μM
Compound: 3c
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs under normoxia condition by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs under normoxia condition by MTT assay
[PMID: 31740202]
HeLa IC50
0.79 μM
Compound: 3c
Inhibition of HIF1alpha transcriptional activity in human HeLa cells transfected with HRE-firefly luciferase and cytomegalovirus-promoter expressing Renilla luciferase incubated for 12 hrs under 1% O2 condition by dual luciferase reporter gene assay
Inhibition of HIF1alpha transcriptional activity in human HeLa cells transfected with HRE-firefly luciferase and cytomegalovirus-promoter expressing Renilla luciferase incubated for 12 hrs under 1% O2 condition by dual luciferase reporter gene assay
[PMID: 31740202]
Molecular Weight

260.29

Formula

C17H12N2O
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C(C1=C2C=NN1C3=CC=C(C)C=C3)C4=C2C=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (38.42 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8419 mL 19.2093 mL 38.4187 mL
5 mM 0.7684 mL 3.8419 mL 7.6837 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8419 mL 19.2093 mL 38.4187 mL 96.0467 mL
5 mM 0.7684 mL 3.8419 mL 7.6837 mL 19.2093 mL
10 mM 0.3842 mL 1.9209 mL 3.8419 mL 9.6047 mL
15 mM 0.2561 mL 1.2806 mL 2.5612 mL 6.4031 mL
20 mM 0.1921 mL 0.9605 mL 1.9209 mL 4.8023 mL
25 mM 0.1537 mL 0.7684 mL 1.5367 mL 3.8419 mL
30 mM 0.1281 mL 0.6403 mL 1.2806 mL 3.2016 mL
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HIF-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HIF-IN-12393928-76-0HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHHeLa cellsHIF-1αno obvious cytotoxicityInhibitorinhibitorinhibit

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