2. Cell Cycle/DNA Damage Metabolic Enzyme/Protease
  3. HSP
  4. HM03

HM03 is a potent and selective HSPA5 (Heat shock 70kDa protein 5, also known as Bip, Grp78) inhibitor. HM03 has anticancer activity.

For research use only. We do not sell to patients.

HM03 Chemical Structure

HM03 Chemical Structure

CAS No. : 500565-15-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 80 In-stock
25 mg USD 160 In-stock
50 mg USD 260 In-stock
100 mg USD 420 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of HM03:

HM03 Related Antibodies

Top Publications Citing Use of Products

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HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HM03 is a potent and selective HSPA5 (Heat shock 70kDa protein 5, also known as Bip, Grp78) inhibitor. HM03 has anticancer activity[1].

IC50 & Target[1]

HSPA5

 

In Vitro

HM03 (0.1-50 μM; 72 hours) exhibits over 50% inhibition at 25 μM concentration in HCT116 cells[1].
? HM03 forms more binding interactions with HSPA5 and HSPA9 than with the other HSP70 proteins[1].
? HM03 exhibits promising inhibition activities from cancer cell viability and tumor inhibition assays[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HM03 Related Antibodies

Cell Viability Assay[1]

Cell Line: HCT116 cells
Concentration: 0.1, 1, 10, 25, 50 μM
Incubation Time: 72 hours
Result: Exhibited prominent inhibition effect (18% survival at 25 µM).
Molecular Weight

462.97

Formula

C26H27ClN4O2
CAS No.
Appearance

Solid

Color

Pink to red

SMILES

OC1=CC=C(NC2=C(C=C(OC)C=C3)C3=NC4=CC(Cl)=CC=C42)C=C1CN5CCN(C)CC5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (270.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1600 mL 10.7998 mL 21.5997 mL
5 mM 0.4320 mL 2.1600 mL 4.3199 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (13.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1600 mL 10.7998 mL 21.5997 mL 53.9992 mL
5 mM 0.4320 mL 2.1600 mL 4.3199 mL 10.7998 mL
10 mM 0.2160 mL 1.0800 mL 2.1600 mL 5.3999 mL
15 mM 0.1440 mL 0.7200 mL 1.4400 mL 3.5999 mL
20 mM 0.1080 mL 0.5400 mL 1.0800 mL 2.7000 mL
25 mM 0.0864 mL 0.4320 mL 0.8640 mL 2.1600 mL
30 mM 0.0720 mL 0.3600 mL 0.7200 mL 1.8000 mL
40 mM 0.0540 mL 0.2700 mL 0.5400 mL 1.3500 mL
50 mM 0.0432 mL 0.2160 mL 0.4320 mL 1.0800 mL
60 mM 0.0360 mL 0.1800 mL 0.3600 mL 0.9000 mL
80 mM 0.0270 mL 0.1350 mL 0.2700 mL 0.6750 mL
100 mM 0.0216 mL 0.1080 mL 0.2160 mL 0.5400 mL
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HM03 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HM03500565-15-1HM 03HM-03HSPHeat shock proteinsheatshockproteincancercellHCT116tumorInhibitorinhibitorinhibit

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