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  5. Imiquimod hydrochloride

Imiquimod hydrochloride  (Synonyms: R 837 hydrochloride)

Cat. No.: HY-B0180A Purity: 99.74%
COA Handling Instructions

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Imiquimod hydrochloride (R 837 hydrochloride), an immune response modifier, is a selective toll like receptor 7 (TLR7) agonist. Imiquimod hydrochloride exhibits antiviral and antitumor effects in vivo. Imiquimod hydrochloride can be used for the research of external genital, perianal warts, cancer and COVID-19.

For research use only. We do not sell to patients.

Imiquimod hydrochloride Chemical Structure

Imiquimod hydrochloride Chemical Structure

CAS No. : 99011-78-6

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10 mM * 1 mL in DMSO
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Based on 39 publication(s) in Google Scholar

Other Forms of Imiquimod hydrochloride:

Imiquimod hydrochloride Related Antibodies

Top Publications Citing Use of Products

38 Publications Citing Use of MCE Imiquimod hydrochloride

WB

    Imiquimod hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2016 May 25;7:11724.  [Abstract]

    Freshly prepared splenic B cells are left unstimulated (lanes 1 and 4), or stimulated by 10 μg/mL αIgM for 6 or 24 h (lanes 2 and 3), or by 1 μg/mL IMQ for 6, 24, 48 and 72 h (lanes 5-8), or by αIgM and IMQ for 24 or 48 h (lanes 9+10), respectively.

    View All Toll-like Receptor (TLR) Isoform Specific Products:

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    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    View All HSV Isoform Specific Products:

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Imiquimod hydrochloride (R 837 hydrochloride), an immune response modifier, is a selective toll like receptor 7 (TLR7) agonist. Imiquimod hydrochloride exhibits antiviral and antitumor effects in vivo. Imiquimod hydrochloride can be used for the research of external genital, perianal warts, cancer and COVID-19[1][2].

    IC50 & Target[1][3]

    TLR7

     

    HSV-1

     

    In Vivo

    In animal models, Imiquimod hydrochloride stimulates the innate immune response by increasing NK cell activity, activating macrophages to secrete cytokines and nitric oxide, and inducing proliferation and differentiation of B lymphocytes. Imiquimod hydrochloride stimulates the innate immune response through induction, synthesis, and release of cytokines, including interferon-a (IFN-α), interleukin (IL)-6, and tumour necrosis factor (TNF)-α[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    276.76

    Formula

    C14H17ClN4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC1=NC2=CC=CC=C2C3=C1N=CN3CC(C)C.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    Methanol : 24 mg/mL (86.72 mM; Need ultrasonic)

    H2O : 8.33 mg/mL (30.10 mM; Need ultrasonic)

    DMSO : 8 mg/mL (28.91 mM; ultrasonic and warming and heat to 50°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 3.85 mg/mL (13.91 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6132 mL 18.0662 mL 36.1324 mL
    5 mM 0.7226 mL 3.6132 mL 7.2265 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.8 mg/mL (2.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.8 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 0.8 mg/mL (2.89 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 0.8 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.77%

    COA (255 KB) HNMR (237 KB) LCMS (121 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO / H2O / Methanol 1 mM 3.6132 mL 18.0662 mL 36.1324 mL 90.3310 mL
    5 mM 0.7226 mL 3.6132 mL 7.2265 mL 18.0662 mL
    10 mM 0.3613 mL 1.8066 mL 3.6132 mL 9.0331 mL
    DMSO / H2O / Methanol 15 mM 0.2409 mL 1.2044 mL 2.4088 mL 6.0221 mL
    20 mM 0.1807 mL 0.9033 mL 1.8066 mL 4.5165 mL
    25 mM 0.1445 mL 0.7226 mL 1.4453 mL 3.6132 mL
    H2O / Methanol 30 mM 0.1204 mL 0.6022 mL 1.2044 mL 3.0110 mL
    Methanol 40 mM 0.0903 mL 0.4517 mL 0.9033 mL 2.2583 mL
    50 mM 0.0723 mL 0.3613 mL 0.7226 mL 1.8066 mL
    60 mM 0.0602 mL 0.3011 mL 0.6022 mL 1.5055 mL
    80 mM 0.0452 mL 0.2258 mL 0.4517 mL 1.1291 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Imiquimod hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Imiquimod99011-78-6R 837R837R-837Toll-like Receptor (TLR)AutophagySARS-CoVHSVSARS coronavirusHerpes simplex virushumanpapillomaviruswartsmolluscumcontagiosumactinickeratosisbasal ,cellcarcinomasquamouscellBowen'sdiseasenongenitalinfectionvulvarintraepithelialneoplasiaInhibitorinhibitorinhibit

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