Indole

Name: Indole
Brand: MedChemExpress
SKU: HY-W001132
Rating: 4.5 (1 reviews)

Cat. No.: HY-W001132 Purity: 99.97%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Indole is an aromatic, heterocyclic, organic compound which widely distributed in the natural environment and can be produced by a variety of bacteria. Indole regulates various aspects of bacterial physiology, including spore formation, plasmid stability, resistance to drugs, biofilm formation, and virulence as an intercellular signal molecule.

For research use only. We do not sell to patients.

Indole Chemical Structure

CAS No. : 120-72-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Indole:

Indole-d₆ Get quote
Indole-¹³C Get quote
Indole-¹³C₈,¹⁵N Get quote
Indole-¹⁵N Get quote
Indole-d₇ Get quote
Indole (standard) Get quote

1 Publications Citing Use of MCE Indole

•Imeta. 2024 Jan 15.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 2	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 22386528]
HEp-2	IC₅₀	> 50 μM Compound: 24	Cytotoxicity against human Hep2 cells after 72 hrs by MTT assay Cytotoxicity against human Hep2 cells after 72 hrs by MTT assay	[PMID: 27116711]
HL-60	IC₅₀	> 50 μM Compound: 24	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 27116711]
MDA-MB-231	IC₅₀	> 100 μM Compound: 2	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 22386528]
MRC5	IC₅₀	15.58 μM Compound: 2	Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay	[PMID: 22386528]
NCI-H292	IC₅₀	> 50 μM Compound: 24	Cytotoxicity against human NCI-H292 cells after 72 hrs by MTT assay Cytotoxicity against human NCI-H292 cells after 72 hrs by MTT assay	[PMID: 27116711]
SK-N-SH	IC₅₀	1.78 μM Compound: 2	Cytotoxicity against human SK-N-SH cells after 48 hrs by MTT assay Cytotoxicity against human SK-N-SH cells after 48 hrs by MTT assay	[PMID: 22386528]
U-251	EC₅₀	> 625 μM Compound: Indole	Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U251MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]
U-87MG ATCC	EC₅₀	> 625 μM Compound: Indole	Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human U87MG cells after 72 hrs by sulforhodamine B assay	[PMID: 30568753]

In Vitro

Indole (2 μM, 48 h) inhibits cell proliferation and cell connection damage in HCT116 and SW620 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HCT116, SW620
Concentration:	2 μM
Incubation Time:	48 h
Result:	Inhibited cell growth and moderated cell cycle progression from G1 to S phase in CRC cell lines HCT116 and SW620.

Western Blot Analysis^[2]

Cell Line:	HCT116, SW620
Concentration:	2 μM
Incubation Time:	48 h
Result:	Increased protein expressions of occludin and claudin-3.

Clinical Trial

Molecular Weight

117.15

Formula

C₈H₇N

CAS No.

120-72-9

Appearance

Solid

Color

White to off-white

SMILES

C1(C=CC=C2)=C2NC=C1

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (853.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 3.57 mg/mL (30.47 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.5360 mL	42.6800 mL	85.3599 mL
5 mM	1.7072 mL	8.5360 mL	17.0720 mL
10 mM	0.8536 mL	4.2680 mL	8.5360 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (23.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (23.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (23.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (280 KB) SDS (624 KB)

COA (254 KB) HNMR (133 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. Lee JH, et al. Indole as an intercellular signal in microbial communities. FEMS Microbiol Rev. 2010 Jul;34(4):426-44. [Content Brief]

[2]. Wang L, et al. Black rice diet alleviates colorectal cancer development through modulating tryptophan metabolism and activating AHR pathway. Imeta. 2024 Jan 15;3(1):e165. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.5360 mL	42.6800 mL	85.3599 mL	213.3998 mL
	5 mM	1.7072 mL	8.5360 mL	17.0720 mL	42.6800 mL
	10 mM	0.8536 mL	4.2680 mL	8.5360 mL	21.3400 mL
	15 mM	0.5691 mL	2.8453 mL	5.6907 mL	14.2267 mL
	20 mM	0.4268 mL	2.1340 mL	4.2680 mL	10.6700 mL
	25 mM	0.3414 mL	1.7072 mL	3.4144 mL	8.5360 mL
	30 mM	0.2845 mL	1.4227 mL	2.8453 mL	7.1133 mL
DMSO	40 mM	0.2134 mL	1.0670 mL	2.1340 mL	5.3350 mL
	50 mM	0.1707 mL	0.8536 mL	1.7072 mL	4.2680 mL
	60 mM	0.1423 mL	0.7113 mL	1.4227 mL	3.5567 mL
	80 mM	0.1067 mL	0.5335 mL	1.0670 mL	2.6675 mL
	100 mM	0.0854 mL	0.4268 mL	0.8536 mL	2.1340 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Indole Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indole120-72-9Endogenous Metaboliteorganic compoundintercellular signal moleculespore formationplasmid stabilitydrug resistancebiofilm formationvirulenceInhibitorinhibitorinhibit

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Indole

Customer Review

Other Forms of Indole:

Indole Related Antibodies

1 Publications Citing Use of MCE Indole

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Indole Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indole Related Classifications

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