K145 hydrochloride

Name: K145 hydrochloride
Brand: MedChemExpress
SKU: HY-15779A
Rating: 5.0 (8 reviews)

Cat. No.: HY-15779A Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

K145 hydrochloride is a selective, substrate-competitive and orally active SphK2 inhibitor with an IC₅₀ of 4.3 μM and a K_i of 6.4 μM. K145 hydrochloride is inactive against SphK1 and other protein kinases. K145 hydrochloride induces cell apoptosis and has potently antitumor activity.

For research use only. We do not sell to patients.

K145 hydrochloride Chemical Structure

CAS No. : 1449240-68-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 121	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 121	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 466	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of K145 hydrochloride:

K145 Get quote

6 Publications Citing Use of MCE K145 hydrochloride

: K145 hydrochloride purchased from MedChemExpress. Usage Cited in: Am J Cancer Res. 2019 Mar 1;9(3):546-561. [Abstract]; Western immunoblotting analysis of NOXA protein levels in RBE and HCCC9810 cells treated with different concentrations of K145 for 24 h. Data shown represents 2 independent experiments.

: K145 hydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2017 Nov 4;493(1):286-290. [Abstract]; Inhibition of pAkt by API-2 effectively prevents K145 induced increasing phosphorylation of FoxO1 in response to insulin. API-2 also significantly reverses K145 suppressed PEPCK and G6Pase mRNA and protein expression.

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SphK1 SphK2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

SphK2

In Vitro

K145 (0-10 μM; 24-72 hours; U937 cells) treatment significantly inhibits the growth of U937 cells in a concentration-dependent manner^[1].
K145 (10 μM; 24 hours; U937 cells) treatment significantly induces apoptosis in U937 cells^[1].
K145 (4-8 μM; 3 hours; U937 cells) treatment decreases the phosphorylation of ERK and Akt^[1].
Treatment with K145 (10 μM) causes a decrease of total cellular S1P without significant effects on ceramide levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	U937 cells
Concentration:	0 µM, 4 µM, 6 µM, 8 µM, 10 µM
Incubation Time:	24 hours, 48 hours, 72 hours
Result:	Significantly inhibited the growth of U937 cells in a concentration-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	U937 cells
Concentration:	10 µM
Incubation Time:	24 hours
Result:	Significantly induced apoptosis in U937 cells.

Western Blot Analysis^[1]

Cell Line:	U937 cells
Concentration:	4 µM, 8 µM
Incubation Time:	3 hours
Result:	Phosphorylated ERK and Akt were decreased.

In Vivo

K145 (50 mg/kg; oral gavage; daily; for 15 days; BALB/c-nu mice) treatment significantly inhibits the growth of U937 tumors in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c-nu mice injected with U937 cells^[1]
Dosage:	50 mg/kg
Administration:	Oral gavage; daily; for 15 days
Result:	Inhibited the growth of U937 tumors at 50 mg/kg dose and no apparent toxicity was observed.

Molecular Weight

384.92

Formula

C₁₈H₂₅ClN₂O₃S

CAS No.

1449240-68-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(N(CCN)C/1=O)SC1=C/CCC2=CC=C(OCCCC)C=C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (129.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5979 mL	12.9897 mL	25.9794 mL
5 mM	0.5196 mL	2.5979 mL	5.1959 mL
10 mM	0.2598 mL	1.2990 mL	2.5979 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (245 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Liu K, et al. Biological characterization of 3-(2-amino-ethyl)-5-[3-(4-butoxyl-phenyl)-propylidene]-thiazolidine-2,4-dione (K145) as a selective sphingosine kinase-2 inhibitor and anticancer agent. PLoS One. 2013;8(2):e56471. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5979 mL	12.9897 mL	25.9794 mL	64.9486 mL
	5 mM	0.5196 mL	2.5979 mL	5.1959 mL	12.9897 mL
	10 mM	0.2598 mL	1.2990 mL	2.5979 mL	6.4949 mL
	15 mM	0.1732 mL	0.8660 mL	1.7320 mL	4.3299 mL
	20 mM	0.1299 mL	0.6495 mL	1.2990 mL	3.2474 mL
	25 mM	0.1039 mL	0.5196 mL	1.0392 mL	2.5979 mL
	30 mM	0.0866 mL	0.4330 mL	0.8660 mL	2.1650 mL
	40 mM	0.0649 mL	0.3247 mL	0.6495 mL	1.6237 mL
	50 mM	0.0520 mL	0.2598 mL	0.5196 mL	1.2990 mL
	60 mM	0.0433 mL	0.2165 mL	0.4330 mL	1.0825 mL
	80 mM	0.0325 mL	0.1624 mL	0.3247 mL	0.8119 mL
	100 mM	0.0260 mL	0.1299 mL	0.2598 mL	0.6495 mL