L-Asparagine monohydrate

Name: L-Asparagine monohydrate
Brand: MedChemExpress
SKU: HY-W017443
Rating: 5.0 (3 reviews)

Cat. No.: HY-W017443 Purity: 98.67%: Data Sheet
SDS

COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

L-Asparagine monohydrate is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine monohydrate can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.

For research use only. We do not sell to patients.

L-Asparagine monohydrate Chemical Structure

CAS No. : 5794-13-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of L-Asparagine monohydrate:

L-Asparagine In-stock
L-Asparagine-¹³C₄,¹⁵N₂ monohydrate Get quote
L-Asparagine-amide-¹⁵N monohydrate Get quote
L-Asparagine-¹³C₄,¹⁵N₂,d₃ monohydrate Get quote
L-Asparagine-¹⁵N₂,d₃ monohydrate Get quote
L-Asparagine-1,2,3,4-¹³C₄ monohydrate Get quote
L-Asparagine monohydrate (Standard) Get quote

3 Publications Citing Use of MCE L-Asparagine monohydrate

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Microbial Metabolite

In Vitro

L-Asparagine monohydrate is the biological substrate of L-Asparaginase. The potential side effects of L-Asparaginase are causing normal cell death and inducing inflammation, metabolic disorders and neurotoxicity after use. The toxic side effects of L-Asparaginase may be caused by the action of glutaminase in it^[1].
L-Asparagine monohydrate and L-Asparaginase have also been used to develop biosensors for analyzing asparagine levels in leukemia or food industry. In application, the characteristics of L-Asparagine monohydrate hydrolysis, which produces ammonium ions and causes pH changes, can be used to determine the level of L-Asparagine monohydrate by color and absorbance changes^[1].
L-Asparaginase can preferentially kill leukemia cells, and the leukemia cell killing rate can be accurately measured using leukemia cell surface markers. L-Asparaginase showed no significant dose response at doses of 0.0001-0.1 IU/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-Asparagine monohydrate is widely found in bacteria, fungi, actinomycetes, plants and animals. L-Asparagine monohydrate from Escherichia coli (E. coli) and Enterobacter chrysanthemi (E. chrysanthemi) has been used to acute lymphoblastic leukemia (ALL) research, and has made progress in the subcutaneous Gardner 6C3HED lymphosarcoma model established in mice^[1].
In the NOD.Cg-PRKDC^scidIL2RG^tm1Wjl (NSG) mouse model, L-Asparaginase (1000 or 5000 IU/kg; intraperitoneal injection; once a day for 3 consecutive days) significantly depletes L-Asparagine monohydrate in the blood; in the high-dose group, L-Asparagine monohydrate also recoveres slowly after the cessation of L-Asparaginase injection^[3].
When L-Asparaginase is encapsulated in red blood cells (ASP-RBC) and given to Swiss mice by intravenous injection (200 or 1000 IU/kg), the depletion time of L-Asparagine monohydrate in their plasma is prolonged. In the 1000 IU/kg dose group, levels of L-Asparagine monohydrate remains undetectable for up to 14 days, and the half-life of the enzyme is 9-10 days^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

150.13

Formula

C₄H₁₀N₂O₄

CAS No.

5794-13-8

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](C(O)=O)CC(N)=O.[H]O[H]

Structure Classification

Amino acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 20 mg/mL (133.22 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6609 mL	33.3045 mL	66.6089 mL
5 mM	1.3322 mL	6.6609 mL	13.3218 mL
10 mM	0.6661 mL	3.3304 mL	6.6609 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Purity & Documentation

Purity: ≥99.0%

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Data Sheet (276 KB) SDS (393 KB)

COA (247 KB)

Handling Instructions (2659 KB)

References

[1]. Batool T, et al. A Comprehensive Review on L-Asparaginase and Its Applications. Appl Biochem Biotechnol. 2016 Mar;178(5):900-23. [Content Brief]

[2]. Asselin BL, et al. In vitro and in vivo killing of acute lymphoblastic leukemia cells by L-asparaginase. Cancer Res. 1989 Aug 1;49(15):4363-8. [Content Brief]

[3]. Horvath TD, et al. Assessment of l-Asparaginase Pharmacodynamics in Mouse Models of Cancer. Metabolites. 2019 Jan 9;9(1):10. [Content Brief]

[4]. Kravtzoff R, et al. Erythrocytes as carriers for L-asparaginase. Methodological and mouse in-vivo studies. J Pharm Pharmacol. 1990 Jul;42(7):473-6. [Content Brief]

[5]. Ilaria M Michelozzi, et al. Acute myeloid leukaemia niche regulates response to L-asparaginase. Br J Haematol. 2019 Aug;186(3):420-430. doi: 10.1111/bjh.15920. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	6.6609 mL	33.3045 mL	66.6089 mL	166.5223 mL
	5 mM	1.3322 mL	6.6609 mL	13.3218 mL	33.3045 mL
	10 mM	0.6661 mL	3.3304 mL	6.6609 mL	16.6522 mL
	15 mM	0.4441 mL	2.2203 mL	4.4406 mL	11.1015 mL
	20 mM	0.3330 mL	1.6652 mL	3.3304 mL	8.3261 mL
	25 mM	0.2664 mL	1.3322 mL	2.6644 mL	6.6609 mL
	30 mM	0.2220 mL	1.1101 mL	2.2203 mL	5.5507 mL
	40 mM	0.1665 mL	0.8326 mL	1.6652 mL	4.1631 mL
	50 mM	0.1332 mL	0.6661 mL	1.3322 mL	3.3304 mL
	60 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7754 mL
	80 mM	0.0833 mL	0.4163 mL	0.8326 mL	2.0815 mL
	100 mM	0.0666 mL	0.3330 mL	0.6661 mL	1.6652 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.