2. Anti-infection Apoptosis Autophagy Epigenetics PI3K/Akt/mTOR Cell Cycle/DNA Damage
  3. Bacterial Apoptosis Antibiotic Autophagy AMPK Eukaryotic Initiation Factor (eIF)
  4. Maduramicin ammonium

Maduramicin ammonium  (Synonyms: Maduramycin ammonium)

Cat. No.: HY-N7071A Purity: 99.89%
SDS
COA
Handling Instructions Technical Support

Maduramicin (Maduramycin) ammonium is isolated from the actinomycete Actinomadura rubra. Maduramicin ammonium is an oral active anticoccidial agent used in research related to Eimeria, Adenosarcina, Enterococcus faecalis, and Enterococcus disperitus infections. Maduramicin ammonium induces apoptosis (apoptosis) in chicken cardiomyocytes through both intrinsic and extrinsic pathways and also impairs autophagic flux and induces rhabdomyolysis by activating the AMPK-mediated eIF2α-ATF4 endoplasmic reticulum stress pathway.

For research use only. We do not sell to patients.

Maduramicin ammonium Chemical Structure

Maduramicin ammonium Chemical Structure

CAS No. : 84878-61-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Other Forms of Maduramicin ammonium:

Maduramicin ammonium Related Antibodies

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Description

Maduramicin (Maduramycin) ammonium is isolated from the actinomycete Actinomadura rubra. Maduramicin ammonium is an oral active anticoccidial agent used in research related to Eimeria, Adenosarcina, Enterococcus faecalis, and Enterococcus disperitus infections. Maduramicin ammonium induces apoptosis (apoptosis) in chicken cardiomyocytes through both intrinsic and extrinsic pathways and also impairs autophagic flux and induces rhabdomyolysis by activating the AMPK-mediated eIF2α-ATF4 endoplasmic reticulum stress pathway[1][2][3][4].

IC50 & Target

IC50: bacterial; apoptosis[1]
In Vitro

Maduramicin ammonium (0.5-2 μM, 10 h) induces endoplasmic reticulum stress in C2C12 and L6 cells by activating the eIF2α-ATF4 signaling pathway[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Maduramicin ammonium Related Antibodies

Western Blot Analysis[4]

Cell Line: C2C12 (mouse myoblasts), L6 (rat myoblasts)
Concentration: 0.5, 1, 2 μM
Incubation Time: 10 hours
Result: Dose-dependently increased eIF2α phosphorylation and ATF4 expression, indicating activation of endoplasmic reticulum stress.
In Vivo

Maduramicin ammonium (3.5 and 7 mg/kg, oral administration, once daily for 7 days) induced rhabdomyolysis in ICR mice, leading to weight loss, elevated serum biochemical markers, and damage to the liver, kidney, myocardium, and skeletal muscle[3].
Maduramicin ammonium (1.75–7 mg/kg, oral administration, once daily for 7 days) impaired autophagic flux by activating the AMPK-mediated eIF2α-ATF4 endoplasmic reticulum stress pathway in Wistar rats, thereby inducing skeletal muscle injury[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mouse (Maduramicin-induced rhabdomyolysis model)[3]
Dosage: 3.5, 7 mg/kg
Administration: Oral gavage (p.o.), once daily for 7 days
Result: Significantly reduced body weight, increased AST, ALT, LDH, BUN, and CK levels;
Caused that liver exhibited vacuolar degeneration, kidney showed renal tubular epithelial cell shedding, and myocardial and skeletal muscle fibers were disorganized and partially lysed.
Animal Model: Wistar rats (Maduramicin-induced skeletal muscle injury model)
Dosage: 1.75, 3.5, 7 mg/kg
Administration: Oral gavage (p.o.), once daily for 7 days
Result: Dose-dependently increased LC3-II and p62 protein levels in skeletal muscle, indicating impaired autophagic flux;
Upregulated eIF2α phosphorylation and ATF4 expression, activating endoplasmic reticulum stress;
Increased AMPK phosphorylation, suggesting activation of the AMPK signaling pathway.
Molecular Weight

934.16

Formula

C47H83NO17
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

C[C@]1([C@]2([H])O[C@](C)([C@@]3([H])[C@H](C[C@@]([C@@]4([H])O[C@](O)([C@H](C)C[C@@H]4C)C)([H])O3)O[C@]5([H])C[C@@H]([C@@H](OC)[C@H](C)O5)OC)CC2)O[C@]6(O[C@]([C@H](C)[C@@H](O)C6)([H])[C@H]([C@@]7([H])[C@@H]([C@H]([C@H](C)[C@](CC(O)=O)(O)O7)OC)OC)C)CC1.N
Structure Classification
Initial Source

Streptomyces
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (17.84 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0705 mL 5.3524 mL 10.7048 mL
5 mM 0.2141 mL 1.0705 mL 2.1410 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (1.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.67 mg/mL (1.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 1.67 mg/mL (1.79 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

SDS (419 KB)

COA (256 KB) HNMR (333 KB) RP-HPLC (241 KB) MS (69 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0705 mL 5.3524 mL 10.7048 mL 26.7620 mL
5 mM 0.2141 mL 1.0705 mL 2.1410 mL 5.3524 mL
10 mM 0.1070 mL 0.5352 mL 1.0705 mL 2.6762 mL
15 mM 0.0714 mL 0.3568 mL 0.7137 mL 1.7841 mL
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Maduramicin ammonium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Maduramicin84878-61-5MaduramycinBacterialApoptosisAntibioticAutophagyAMPKEukaryotic Initiation Factor (eIF)AMP-activated protein kinasecoccidiosis?Eimeria meleagrimitisE. adenoeidesE. gallopavonisE. dispersaInhibitorinhibitorinhibit

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