2. Metabolic Enzyme/Protease
  3. Cytochrome P450
  4. Mephenytoin

Mephenytoin, an anticonvulsant, is the CYP2C19 and CYP2B6 substrate.

For research use only. We do not sell to patients.

Mephenytoin Chemical Structure

Mephenytoin Chemical Structure

CAS No. : 50-12-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 182 In-stock
Solution
10 mM * 1 mL in DMSO USD 182 In-stock
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5 mg USD 106 In-stock
10 mg USD 165 In-stock
50 mg USD 275 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Mephenytoin:

Mephenytoin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Mephenytoin

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms
CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Mephenytoin, an anticonvulsant, is the CYP2C19 and CYP2B6 substrate[1].

IC50 & Target

CYP2

 

In Vivo

Mephenytoin (orally administration, 100 mg/kg, 200 mg/kg) can reduce maternal weight gain and increase offspring mortality at 200 mg/kg but not produce excessive offspring mortality at 100 mg/kg in Pregnant Sprague-Dawley CD rats[2].
Mephenytoin (i.p., 20 mg/kg per day for 16 days) significantly reduces serum cholesterol and triglyceride levels in mice, which may have a hypolipidemic effect[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

218.26

Formula

C12H14N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(C)C(C(C2=CC=CC=C2)(CC)N1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (229.09 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5818 mL 22.9089 mL 45.8178 mL
5 mM 0.9164 mL 4.5818 mL 9.1636 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.96%

SDS (393 KB)

COA (254 KB) HNMR (245 KB) LCMS (255 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5818 mL 22.9089 mL 45.8178 mL 114.5444 mL
5 mM 0.9164 mL 4.5818 mL 9.1636 mL 22.9089 mL
10 mM 0.4582 mL 2.2909 mL 4.5818 mL 11.4544 mL
15 mM 0.3055 mL 1.5273 mL 3.0545 mL 7.6363 mL
20 mM 0.2291 mL 1.1454 mL 2.2909 mL 5.7272 mL
25 mM 0.1833 mL 0.9164 mL 1.8327 mL 4.5818 mL
30 mM 0.1527 mL 0.7636 mL 1.5273 mL 3.8181 mL
40 mM 0.1145 mL 0.5727 mL 1.1454 mL 2.8636 mL
50 mM 0.0916 mL 0.4582 mL 0.9164 mL 2.2909 mL
60 mM 0.0764 mL 0.3818 mL 0.7636 mL 1.9091 mL
80 mM 0.0573 mL 0.2864 mL 0.5727 mL 1.4318 mL
100 mM 0.0458 mL 0.2291 mL 0.4582 mL 1.1454 mL
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Mephenytoin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mephenytoin50-12-4Cytochrome P450CYPsCYP2C19CYP2B6phenotypingsubstrateleukopeniaInhibitorinhibitorinhibit

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