2. Autophagy PI3K/Akt/mTOR
  3. Autophagy PI3K mTOR Akt
  4. Methyl Eugenol

Methyl Eugenol is a bait that has oral activity against oriental fruit fly (Hendel).Methyl Eugenol has anti-cancer and anti-inflammatory activities. Methyl Eugenol can induce Autophagy in cells. Methyl Eugenol can be used in the study of intestinal ischemia/reperfusion injury.

For research use only. We do not sell to patients.

Methyl Eugenol Chemical Structure

Methyl Eugenol Chemical Structure

CAS No. : 93-15-2

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Liquid
100 mg USD 50 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Methyl Eugenol:

Methyl Eugenol Related Antibodies

Top Publications Citing Use of Products

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All mTOR Isoform Specific Products:

View All Isoforms
mTOR mTORC1 mTORC2

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Methyl Eugenol is a bait that has oral activity against oriental fruit fly (Hendel).Methyl Eugenol has anti-cancer and anti-inflammatory activities. Methyl Eugenol can induce Autophagy in cells. Methyl Eugenol can be used in the study of intestinal ischemia/reperfusion injury[1][2][3].

In Vitro

Methyl Eugenol (25% (2 mL)-100% (8 mL)) is able to trap the orange fly in a dose-dependent manner [1].
Methyl Eugenol (3.12-200 μM; 48 h) can inhibit cell proliferation (IC50=50 μM) in human retinoblastoma RB355 cells by inducing (50 μM; 24 h) autophagy and inhibiting (50 μM; 48 h) the PI3K/mTOR/Akt signaling pathway in cells[2].
Methyl Eugenol (25-100 μM) can arrest the cell cycle of RB355 cells at the G2/M phase[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methyl Eugenol Related Antibodies

Cell Autophagy Assay[2]

Cell Line: RB355 (Human retinoblastoma cells)
Concentration: 50 μM
Incubation Time: 24 h
Result: Significantly increased the expression of LC3-II in a dose-dependent manner and decreased the expression of p62.
Weakened the inhibitory effect of the autophagy inhibitor gastrostatin.

Western Blot Analysis[2]

Cell Line: RB355 (Human retinoblastoma cells)
Concentration: 50 μM
Incubation Time: 48 h
Result: Inhibited the PI3K/mTOR/Akt signaling pathway.
Down-regulated the expression of m-TOR and pm-TOR proteins in a concentration-dependent manner.
Down-regulated the expression of PI3K/Akt protein.
In Vivo

Methyl Eugenol (100 mg/kg; Oral intubation; Once daily for 30 days) has anti-inflammatory effects in Wistar rats with intestinal ischemia/reperfusion injury (I/R), and can alleviate intestinal ischemia/reperfusion injury[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult Wistar rats (Rattus norvegicus) model of intestinal ischemia/reperfusion injury[3]
Dosage: 100 mg/kg
Administration: Oral intubation; Once daily for 30 days. Before underwent laparotomy with SMA occlusion 30 min of ischemia followed by 60 min of reperfusion.
Result: Significantly decreased the elevated LDH, MDA, and NO levels and concurrently increased the antioxidant biomarkers under test in the intestinal tissue.
Significantly downregulate the mRNA expression levels of TNF-α and IL-6.
Molecular Weight

178.23

Formula

C11H14O2
CAS No.
Appearance

Liquid (Density: 1.036 g/cm3)

Color

Colorless to light yellow

SMILES

COC1=CC(CC=C)=CC=C1OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (561.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.6107 mL 28.0536 mL 56.1073 mL
5 mM 1.1221 mL 5.6107 mL 11.2215 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.79%

COA (246 KB) HNMR (238 KB) LCMS (86 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.6107 mL 28.0536 mL 56.1073 mL 140.2682 mL
5 mM 1.1221 mL 5.6107 mL 11.2215 mL 28.0536 mL
10 mM 0.5611 mL 2.8054 mL 5.6107 mL 14.0268 mL
15 mM 0.3740 mL 1.8702 mL 3.7405 mL 9.3512 mL
20 mM 0.2805 mL 1.4027 mL 2.8054 mL 7.0134 mL
25 mM 0.2244 mL 1.1221 mL 2.2443 mL 5.6107 mL
30 mM 0.1870 mL 0.9351 mL 1.8702 mL 4.6756 mL
40 mM 0.1403 mL 0.7013 mL 1.4027 mL 3.5067 mL
50 mM 0.1122 mL 0.5611 mL 1.1221 mL 2.8054 mL
60 mM 0.0935 mL 0.4676 mL 0.9351 mL 2.3378 mL
80 mM 0.0701 mL 0.3507 mL 0.7013 mL 1.7534 mL
100 mM 0.0561 mL 0.2805 mL 0.5611 mL 1.4027 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Methyl Eugenol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl Eugenol93-15-2AutophagyPI3KmTORAktPhosphoinositide 3-kinaseMammalian target of RapamycinPKBProtein kinase BHuman retinoblastomaRB355Oriental fruit flybait agentIntestinal Ischemia/Reperfusion injury (I/R)cancerWistar ratsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Methyl Eugenol
Cat. No.:
HY-N6996
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.