2. Anti-infection Metabolic Enzyme/Protease Apoptosis
  3. Bacterial MMP Apoptosis Fungal
  4. Methylisothiazolinone

Methylothiazolinone is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone can cause mitochondrial damage in the endothelium of rat cerebral blood vessels.

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Methylisothiazolinone Chemical Structure

Methylisothiazolinone Chemical Structure

CAS No. : 2682-20-4

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Based on 1 publication(s) in Google Scholar

Other Forms of Methylisothiazolinone:

Methylisothiazolinone Related Antibodies

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

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Description

Methylothiazolinone is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone can cause mitochondrial damage in the endothelium of rat cerebral blood vessels[1][2][4].
In Vitro

Methylisothiazolinone (0-8 μg/mL, 24 h) induces cell apoptosis and inflammatory response by activating matrix metalloproteinases (MMPs) in human bronchial epithelial cells[1].
Methylisothiazolinone (0-2.5 μg/mL, 1 h) can cause mitochondrial damage in brain endothelial cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methylisothiazolinone Related Antibodies

Cell Viability Assay[1].

Cell Line: Bronchial epithelial cells (BEAS-2B cells)
Concentration: 0, 2, 4, 8 μg/mL
Incubation Time: 24 h
Result: Caused a decrease in cell viability to 81.0%, 67.9%, and 35.1% at doses of 2, 4, and 8 μg/mL, respectively.

Western Blot Analysis[2].

Cell Line: bEND.3 cell
Concentration: 0, 1, 2.5 μg/mL CMIT/MIT
Incubation Time: 1 h
Result: Increased the level of Drp-1 phosphorylated protein and decreased the level of Mfn-1 phosphorylated protein.
In Vivo

Methylisothiazolinone (0.15 mg/kg; once; i.v.) can increase the susceptibility of rat cerebral vascular endothelium to endogenous pathological stimuli and lead to mitochondrial damage[2].
Methylisothiazolinone (20 or 40 μL; handling skin (20 μL), days 1 and 8; intranasal instillation (40 μL), day 15) can cause sensitization in asthmatic mice when handled on the skin[3].
Methylisothiazolinone (0.1875 mg/kg CMIT/MIT; once daily; 15 days; epidermal contact) promotes the development of atopic dermatitis in mice through Th2/Th17 related immune response dysregulation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male SD rats (230-300 g and 7-8 weeks of age)[2].
Dosage: 0.15 mg/kg
Administration: Intravenous injection (i.v.); once
Result: Resulted in mitochondrial damage and a decrease in tight junction proteins.
Animal Model: Male C57Bl/6 and BALB/c mouse asthma models induced by chemistry (approximately 20 g, 6 weeks old)[3].
Dosage: 20 or 40 μL
Administration: Handled skin (20 μL), on days 1 and 8; intranasal instillation (40 μL), on day 15
Result: Induced an increase in T and B cell proliferation in ear lymph nodes, while producing IFN-γ.
Animal Model: Specific dermatitis (AD) female BALB/c mice established by sensitization of ovalbumin (OVA) epidermis (5 weeks old, n = 5 per group) weighing 16-20 g[4].
Dosage: 0.1875 mg/kg chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT)
Administration: Epidermal contact; once daily; 15 days
Result: Augmented the mRNA expression level of T helper (Th) 2-related cytokines (thymic stromal lymphopoietin, interleukin (IL)-6 and IL-13), Th2 chemokine (chemokine (C-C motif) ligand 17) and the population of CD4IL-4 cells in the skin.
Molecular Weight

115.15

Formula

C4H5NOS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CN1SC=CC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (868.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.6843 mL 43.4216 mL 86.8432 mL
5 mM 1.7369 mL 8.6843 mL 17.3687 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (21.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (21.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.37%

SDS (786 KB)

COA (260 KB) HNMR (245 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 8.6843 mL 43.4216 mL 86.8432 mL 217.1081 mL
5 mM 1.7369 mL 8.6843 mL 17.3686 mL 43.4216 mL
10 mM 0.8684 mL 4.3422 mL 8.6843 mL 21.7108 mL
15 mM 0.5790 mL 2.8948 mL 5.7895 mL 14.4739 mL
20 mM 0.4342 mL 2.1711 mL 4.3422 mL 10.8554 mL
25 mM 0.3474 mL 1.7369 mL 3.4737 mL 8.6843 mL
30 mM 0.2895 mL 1.4474 mL 2.8948 mL 7.2369 mL
40 mM 0.2171 mL 1.0855 mL 2.1711 mL 5.4277 mL
50 mM 0.1737 mL 0.8684 mL 1.7369 mL 4.3422 mL
60 mM 0.1447 mL 0.7237 mL 1.4474 mL 3.6185 mL
80 mM 0.1086 mL 0.5428 mL 1.0855 mL 2.7139 mL
100 mM 0.0868 mL 0.4342 mL 0.8684 mL 2.1711 mL
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Methylisothiazolinone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylisothiazolinone2682-20-4BacterialMMPApoptosisFungalMatrix metalloproteinasessterilizationallergymitochondrial damagedermatitisInhibitorinhibitorinhibit

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