2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Epigenetics Apoptosis Anti-infection
  3. Topoisomerase ADC Cytotoxin MicroRNA Influenza Virus Apoptosis Fungal Antibiotic
  4. Camptothecin

Camptothecin  (Synonyms: Campathecin; (S)-(+)-Camptothecin; CPT)

Cat. No.: HY-16560 Purity: 99.86%
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Camptothecin (CPT), a kind of alkaloid, is a DNA topoisomerase I (Topo I) inhibitor with an IC50 of 679 nM. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression patterns in human cancer cells.

For research use only. We do not sell to patients.

Camptothecin Chemical Structure

Camptothecin Chemical Structure

CAS No. : 7689-03-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 61 In-stock
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10 mM * 1 mL in DMSO USD 61 In-stock
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500 mg USD 165 In-stock
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Based on 42 publication(s) in Google Scholar

Other Forms of Camptothecin:

Camptothecin Related Antibodies

Top Publications Citing Use of Products

38 Publications Citing Use of MCE Camptothecin

WB
IF
IP
Cell Viability Assay

    Camptothecin purchased from MedChemExpress. Usage Cited in: Arch Cancer Res. 2023 Jan 30.

    Camptothecin (CPT; 10-5 -10-10 M; 72 h) demonstrates a strong antitumor activity and an inhibitory of proliferation (IC50 = 25.36 nM) in U937 cells.

    Camptothecin purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2020 Nov;27(11):3162-3176.  [Abstract]

    293T cells transfected with Flag-TopBP1 and GFP-tagged RBMX and its domain (RRM, RBM1CTR, and C-RBD) are treated with 100 nM CPT for 24 h and subjected to immunoprecipitation with Flag-beads. Immunoprecipitates are immunoblotted with Flag and GFP antibodies, respectively.

    Camptothecin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2019 Aug 21;10(1):3761.  [Abstract]

    Genotoxic stress-induced β-catenin signaling is activated via a TCF- or FOXO-independent mechanism. Representative images of the subcellular localization of β-catenin in the indicated cells treated with CPT (10 μM, 1 h), IR (10 Gy), and CDDP (10 μM, 1 h), as analyzed by immunofluorescence staining.

    Camptothecin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 Oct 8;9(1):4139.  [Abstract]

    One hour after treated with UV or 2 h after treated with MMS, Camptothecin (CPT) (10 μM), or Doxorubicin (DOX; 0.5 μM), HEK293T cells expressing HA-SUMO2 and His-RhoB are subjected to sumoylation assay to detect the SUMO2 conjugation of RhoB.

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    • Biological Activity

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    • References

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    Description

    Camptothecin (CPT), a kind of alkaloid, is a DNA topoisomerase I (Topo I) inhibitor with an IC50 of 679 nM[1]. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression patterns in human cancer cells[2][3].

    IC50 & Target[3]

    Topoisomerase I

    679 nM (IC50)

    Camptothecins

     

    In Vitro

    High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward Camptothecin (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50 values of 0.089 μM and 0.067 μM, respectively[4].
    Camptothecin (0.5 μM; 6 and 24 hours) reduces desferrioxamine-activated VEGF expression.
    Camptothecin (0.5 μM; 8 to 24 hours) strongly prevents the desferrioxamine-dependent HIF-1a accumulation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Camptothecin Related Antibodies

    Cell Viability Assay[4]

    Cell Line: MCF7 (Luminal subtype) and HCC1419 (HER2 subtype)
    Concentration: 0.1 μM to 5 μM
    Incubation Time: 72 hours
    Result: High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward CPT (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50 values of 0.089 μM and 0.067 μM, respectively.

    RT-PCR[2]

    Cell Line: HeLa and HEK293 cell lines
    Concentration: 0.5 μmol/L
    Incubation Time: 6 and 24 hours
    Result: Reduces desferrioxamine-activated VEGF expression in both cell lines after 6 and 24 hours of treatment, whereas in normoxic condition camptothecin does not affect the VEGF mRNA level.

    Western Blot Analysis[2]

    Cell Line: HeLa and HEK293 cell lines
    Concentration: 0.5 μmol/L
    Incubation Time: 8 to 24 hours
    Result: Strongly prevents the desferrioxamine-dependent HIF-1a accumulation after 8 to 24 hours, whereas it does not affect HIF-1b levels.
    In Vivo

    Camptothecin (2 mg/kg every other day) treats mice, has developed numerous pulmonary metastases.
    Treatment with both kinase inhibitor of nuclear factor-kappaB-1 (KINK-1) and Camptothecin led to a statistically significant reduction in the number of pulmonary metastases[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL6 mice (injected with B16F10 melanoma cells)[5]
    Dosage: 2 mg/kg
    Administration: every other day, after 19 days
    Result: Has developed numerous pulmonary metastases.
    Clinical Trial
    Molecular Weight

    348.35

    Formula

    C20H16N2O4
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C2=C([C@@](O)(CC)C(OC2)=O)C=C(N1C3)C(C3=C4)=NC5=C4C=CC=C5
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    Solvent & Solubility
    In Vitro: 

    1M NaOH : 10 mg/mL (28.71 mM; ultrasonic and adjust pH to 11 with NaOH)

    DMSO : 6.25 mg/mL (17.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8707 mL 14.3534 mL 28.7068 mL
    5 mM 0.5741 mL 2.8707 mL 5.7414 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Volume (start)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  15% Cremophor EL    85% Saline

      Solubility: 10 mg/mL (28.71 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.86%

    COA (259 KB) HNMR (251 KB) RP-HPLC (242 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / 1M NaOH 1 mM 2.8707 mL 14.3534 mL 28.7068 mL 71.7669 mL
    5 mM 0.5741 mL 2.8707 mL 5.7414 mL 14.3534 mL
    10 mM 0.2871 mL 1.4353 mL 2.8707 mL 7.1767 mL
    15 mM 0.1914 mL 0.9569 mL 1.9138 mL 4.7845 mL
    1M NaOH 20 mM 0.1435 mL 0.7177 mL 1.4353 mL 3.5883 mL
    25 mM 0.1148 mL 0.5741 mL 1.1483 mL 2.8707 mL
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    Camptothecin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Camptothecin7689-03-4Campathecin (S)-(+)-Camptothecin CPTTopoisomeraseADC CytotoxinMicroRNAInfluenza VirusApoptosisFungalAntibioticADC PayloadmiRNAalkaloidDNA topoisomerase ITop1hypoxia-inducible factor-1αHIF-1αmicroRNAsmiRNInhibitorinhibitorinhibit

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