MPP+ iodide

Name: MPP+ iodide
Brand: MedChemExpress
SKU: HY-W008719
Rating: 5.0 (14 reviews)

Cat. No.: HY-W008719 Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MPP+ iodide, a toxic metabolite of the neurotoxin MPTP, causes symptom of Parkinson's disease in animal models by selectively destroying dopaminergic neurons in substantia nigra. MPP+ iodide is taken up by the dopamine transporter into dopaminergic neurons where it exerts its neurotoxic action on mitochondria by affecting complex I of the respiratory chain. MPP+ iodide is also a high affinity substrate for the serotonin transporter (SERT).

For research use only. We do not sell to patients.

MPP+ iodide Chemical Structure

CAS No. : 36913-39-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 88	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 88	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 14 publication(s) in Google Scholar

Other Forms of MPP+ iodide:

MPP+-d₃ iodide Get quote

12 Publications Citing Use of MCE MPP+ iodide

Cell Viability Assay

: MPP+ iodide purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2023 Feb 18. [Abstract]; MPP⁺ iodide (MPP⁺; 0, 0.2, 0.4, 0.6, 0.8, 1, 2 mM; 48 h) decreases viability of SH-SY5Y cells (Fig D). At a concentration of 0.6 mM, MPP⁺ iodide decreases the cell viability by 25%. Moreover, the IC₅₀ value of MPP⁺ iodide on SH-SY5Y cells is 1.233 mM (Fig C).

: MPP+ iodide purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2023 Feb 18. [Abstract]; MPP⁺ iodide (MPP⁺; 1.2 mM; 48 h) decreases TH, Prdx-2, SIRT1 protein levels and increases the ratio of Bax / Bcl-2, in SH-SY5Y cells.

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Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	199 μM Compound: MPP+	Inhibition of the Organic Cation Transporter 3 (OCT3, SLC22A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by MPP+ uptake in HEK-293 JumpIN-SLC22A3 cells Inhibition of the Organic Cation Transporter 3 (OCT3, SLC22A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by MPP+ uptake in HEK-293 JumpIN-SLC22A3 cells	[PMID: 35163125]

In Vitro

MPP+ (1-3 mM; 24 hours) remarkably decreases the viability of cells^[1].
MPP+ also inhibits the activity of nicotinamide adenosine dinucleotide (NADH)-linked respiration in mitochondrial preparations and impairs aerobic glycolysis, leading to the present belief that the inhibition of NADH-linked cell respiration may constitute the final molecular mechanism of MPP+ neurotoxicity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SH-SY5Y cells
Concentration:	1, 2, 3 mM
Incubation Time:	24 hours
Result:	SH-SY5Y cells were treated with MPP+, mimicking the progress of dopaminergic neurons loss in PD; Reduced cell viability in both dose-dependent (1, 2, 3 mM for 24 h) and time dependent (1 mM) manner.

Molecular Weight

297.14

Formula

C₁₂H₁₂IN

CAS No.

36913-39-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

C[N+]1=CC=C(C2=CC=CC=C2)C=C1.[I-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (336.54 mM; Need ultrasonic)

DMSO : 100 mg/mL (336.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3654 mL	16.8271 mL	33.6542 mL
5 mM	0.6731 mL	3.3654 mL	6.7308 mL
10 mM	0.3365 mL	1.6827 mL	3.3654 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (8.41 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (336.54 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (276 KB) SDS (480 KB)

COA (249 KB) HNMR (224 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Zhao M, et al. Mitochondrial calcium dysfunction contributes to autophagic cell death induced by MPP+ via AMPK pathway. Biochem Biophys Res Commun. 2019;509(2):390-394. [Content Brief]

[2]. Martí Y, et al. Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons. Mol Cell Neurosci. 2017;83:37-45. [Content Brief]

[3]. Charlton CG. 1-Methyl-4-phenylpyridinium (MPP+) but not 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) serves as methyl donor for dopamine: a possible mechanism of action. J Geriatr Psychiatry Neurol. 1992;5(2):114-118. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.3654 mL	16.8271 mL	33.6542 mL	84.1354 mL
	5 mM	0.6731 mL	3.3654 mL	6.7308 mL	16.8271 mL
	10 mM	0.3365 mL	1.6827 mL	3.3654 mL	8.4135 mL
	15 mM	0.2244 mL	1.1218 mL	2.2436 mL	5.6090 mL
	20 mM	0.1683 mL	0.8414 mL	1.6827 mL	4.2068 mL
	25 mM	0.1346 mL	0.6731 mL	1.3462 mL	3.3654 mL
	30 mM	0.1122 mL	0.5609 mL	1.1218 mL	2.8045 mL
	40 mM	0.0841 mL	0.4207 mL	0.8414 mL	2.1034 mL
	50 mM	0.0673 mL	0.3365 mL	0.6731 mL	1.6827 mL
	60 mM	0.0561 mL	0.2805 mL	0.5609 mL	1.4023 mL
	80 mM	0.0421 mL	0.2103 mL	0.4207 mL	1.0517 mL
	100 mM	0.0337 mL	0.1683 mL	0.3365 mL	0.8414 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

MPP+ iodide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MPP+36913-39-0Mitochondrial MetabolismdopaminergicneuronscomplexIrespiratorychainneurotoxicInhibitorinhibitorinhibit

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MPP+ iodide

Customer Review

Other Forms of MPP+ iodide:

MPP+ iodide Related Antibodies

12 Publications Citing Use of MCE MPP+ iodide

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Customer Review

MPP+ iodide Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

MPP+ iodide Related Classifications

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