nor-NOHA monoacetate (Synonyms: Nω-Hydroxy-nor-L-arginine monoacetate)

Name: nor-NOHA monoacetate
Brand: MedChemExpress
SKU: HY-112885B
Rating: 5.0 (2 reviews)

Cat. No.: HY-112885B Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

nor-NOHA monoacetate is a selective and reversible arginase inhibitor. nor-NOHA monoacetate induces apoptosis in ARG2-expressing cells under hypoxia. nor-NOHA monoacetate has anti-leukemic activity. nor-NOHA monoacetate can used in study of endothelial dysfunction, immunosuppression and metabolism.

For research use only. We do not sell to patients.

nor-NOHA monoacetate Chemical Structure

CAS No. : 2250019-93-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 570	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 900	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 2 publication(s) in Google Scholar

Other Forms of nor-NOHA monoacetate:

nor-NOHA acetate In-stock
nor-NOHA Get quote
nor-NOHA dihydrochloride Get quote

2 Publications Citing Use of MCE nor-NOHA monoacetate

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References
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Description

In Vitro

nor-NOHA monoacetate (0.1-1 mM, 72 h) induces apoptosis in a dose-dependent manner under hypoxia (1.5% O₂) in K562 cells^[1].
nor-NOHA monoacetate (1 mM, 72 h) attenuates hypoxia-mediated resistance towards imatinib in K562 or KCL22 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

nor-NOHA monoacetate (100 mg/kg, i.v., once time) induces a significant reduction of infarct size in male Sprague-Dawley rats^[2].
nor-NOHA monoacetate (100 mg/kg, i.v., once time) increases plasma citrulline and nitrite levels and decreases the ornithine plasma levels in male Sprague-Dawley rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

236.23

Formula

C₇H₁₆N₄O₅

CAS No.

2250019-93-1

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](C(O)=O)CCNC(NO)=N.O=C(C)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (423.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (211.66 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2332 mL	21.1658 mL	42.3316 mL
5 mM	0.8466 mL	4.2332 mL	8.4663 mL
10 mM	0.4233 mL	2.1166 mL	4.2332 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (423.32 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (247 KB) HNMR (252 KB) LCMS (153 KB)

Handling Instructions (2659 KB)

References

[1]. Ng KP, et al. The arginase inhibitor Nω-hydroxy-nor-arginine (nor-NOHA) induces apoptosis in leukemic cells specifically under hypoxic conditions but CRISPR/Cas9 excludes arginase 2 (ARG2) as the functional target. PLoS One. 2018 Oct 11;13(10):e0205254. [Content Brief]

[2]. Jung C, et al. Arginase inhibition mediates cardioprotection during ischaemia-reperfusion. Cardiovasc Res. 2010 Jan 1;85(1):147-54. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.2332 mL	21.1658 mL	42.3316 mL	105.8291 mL
	5 mM	0.8466 mL	4.2332 mL	8.4663 mL	21.1658 mL
	10 mM	0.4233 mL	2.1166 mL	4.2332 mL	10.5829 mL
	15 mM	0.2822 mL	1.4111 mL	2.8221 mL	7.0553 mL
	20 mM	0.2117 mL	1.0583 mL	2.1166 mL	5.2915 mL
	25 mM	0.1693 mL	0.8466 mL	1.6933 mL	4.2332 mL
	30 mM	0.1411 mL	0.7055 mL	1.4111 mL	3.5276 mL
	40 mM	0.1058 mL	0.5291 mL	1.0583 mL	2.6457 mL
	50 mM	0.0847 mL	0.4233 mL	0.8466 mL	2.1166 mL
	60 mM	0.0706 mL	0.3528 mL	0.7055 mL	1.7638 mL
	80 mM	0.0529 mL	0.2646 mL	0.5291 mL	1.3229 mL
	100 mM	0.0423 mL	0.2117 mL	0.4233 mL	1.0583 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

nor-NOHA monoacetate Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

nor-NOHA monoacetate2250019-93-1Nω-Hydroxy-nor-L-arginine monoacetateArginaseArginase Inhibitornor-NOHAARG2Anti-leukemicImunosuppressionEdothelial dysfunctionInhibitorinhibitorinhibit

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nor-NOHA monoacetate (Synonyms: Nω-Hydroxy-nor-L-arginine monoacetate)

Customer Review

Other Forms of nor-NOHA monoacetate:

nor-NOHA monoacetate Related Antibodies

2 Publications Citing Use of MCE nor-NOHA monoacetate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

nor-NOHA monoacetate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

nor-NOHA monoacetate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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