Nrf2-ARE/hMAO-B/QR2 modulator 1

Name: Nrf2-ARE/hMAO-B/QR2 modulator 1
Brand: MedChemExpress
SKU: HY-144635
Rating: 4.5 (1 reviews)

Cat. No.: HY-144635 Purity: 98.44%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Nrf2-ARE/hMAO-B/QR2 modulator 1 is a Resveratrol-based multitarget-directed ligands with IC₅₀s of 8.05, 9.83 and 0.57 μM for hMAO-B, NRF2 and QR2. Nrf2-ARE/hMAO-B/QR2 modulator 1 has neuroprotection, decreasing ROS production in okadaic acid-treated mice hippocampal slices.

For research use only. We do not sell to patients.

Nrf2-ARE/hMAO-B/QR2 modulator 1 Chemical Structure

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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MAO-A MAO-B

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC₅₀: 8.05 μM (hMAO-B), 9.83 μM (NRF2), 0.57 μM (QR2)^[1]

In Vitro

Nrf2-ARE/hMAO-B/QR2 modulator 1 (compound 4e) (100 μM; 24 hours) reduces the viability of SH-SY5Y cells by around 30%^[1].
Nrf2-ARE/hMAO-B/QR2 modulator 1 (1 μM; 24 hours for pre-treatment, then coincubated for another 24 hours with 10 nM okadaic acid (OA)) significantly increases the cell viability of OA-treated rat primary cortical neurons to 70-80%^[1].
Nrf2-ARE/hMAO-B/QR2 modulator 1 (10 μM) shows the highest expression of young cells (TuJ-1) and mature neurons (MAP-2), as well as the greatest distance of cell migration in mice NSCs which isolated from SGZ^[1].
Nrf2-ARE/hMAO-B/QR2 modulator 1 (1 μM; 72 hours) confers neuroprotection and reduces cell death, decreasing ROS production in mice hippocampal slices exposed to OA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

256.26

Formula

C₁₄H₁₂N₂O₃

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC(/C=C/C2=CC=C(OC)C=C2)=NN1CC#C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (243.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9023 mL	19.5114 mL	39.0229 mL
5 mM	0.7805 mL	3.9023 mL	7.8046 mL
10 mM	0.3902 mL	1.9511 mL	3.9023 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.44%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (247 KB) HNMR (255 KB) LCMS (269 KB)

Handling Instructions (2659 KB)

References

[1]. Herrera-Arozamena C, Estrada-Valencia M, López-Caballero P, et al. Resveratrol-Based MTDLs to Stimulate Defensive and Regenerative Pathways and Block Early Events in Neurodegenerative Cascades. J Med Chem. 2022;65(6):4727-4751. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9023 mL	19.5114 mL	39.0229 mL	97.5572 mL
	5 mM	0.7805 mL	3.9023 mL	7.8046 mL	19.5114 mL
	10 mM	0.3902 mL	1.9511 mL	3.9023 mL	9.7557 mL
	15 mM	0.2602 mL	1.3008 mL	2.6015 mL	6.5038 mL
	20 mM	0.1951 mL	0.9756 mL	1.9511 mL	4.8779 mL
	25 mM	0.1561 mL	0.7805 mL	1.5609 mL	3.9023 mL
	30 mM	0.1301 mL	0.6504 mL	1.3008 mL	3.2519 mL
	40 mM	0.0976 mL	0.4878 mL	0.9756 mL	2.4389 mL
	50 mM	0.0780 mL	0.3902 mL	0.7805 mL	1.9511 mL
	60 mM	0.0650 mL	0.3252 mL	0.6504 mL	1.6260 mL
	80 mM	0.0488 mL	0.2439 mL	0.4878 mL	1.2195 mL
	100 mM	0.0390 mL	0.1951 mL	0.3902 mL	0.9756 mL