1. Anti-infection
  2. Influenza Virus
  3. Oseltamivir

Oseltamivir (GS 4104) is an orally active influenza virus neuraminidase inhibitor (NAI). Oseltamivir inhibits influenza A/H3N2, A/H1N2, A/H1N1, and B viruses with mean IC50s of 0.67, 0.9, 1.34 and 13 nM, respectively.

For research use only. We do not sell to patients.

Oseltamivir Chemical Structure

Oseltamivir Chemical Structure

CAS No. : 196618-13-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 34 publication(s) in Google Scholar

Other Forms of Oseltamivir:

Top Publications Citing Use of Products

33 Publications Citing Use of MCE Oseltamivir

WB

    Oseltamivir purchased from MedChemExpress. Usage Cited in: Lab Invest. 2020 Dec;100(12):1602-1617.  [Abstract]

    After the HBc-positive cells are treated with OP (Oseltamivir phosphate), the phosphorylation of ERK and P65 significantly declined, the expression of IkBα increased, and the expression of nuclear P65 decreased.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Oseltamivir (GS 4104) is an orally active influenza virus neuraminidase inhibitor (NAI). Oseltamivir inhibits influenza A/H3N2, A/H1N2, A/H1N1, and B viruses with mean IC50s of 0.67, 0.9, 1.34 and 13 nM, respectively[1].

    Cellular Effect
    Cell Line Type Value Description References
    A549 CC50
    > 200 μM
    Compound: OSV
    Cytotoxicity against human A549 cells assessed as reduction in cell viability by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability by MTT assay
    [PMID: 31371135]
    Fibroblast CC50
    > 10 μM
    Compound: OS carboxylate
    Cytotoxicity against chicken embryo fibroblast after 2 days by MTS assay
    Cytotoxicity against chicken embryo fibroblast after 2 days by MTS assay
    [PMID: 23664211]
    Fibroblast CC50
    > 20 μM
    Compound: OS
    Cytotoxicity against chicken embryo fibroblasts infected with Influenza A virus (A/Chicken/Shandong/LY/08(H9N2)) preincubated for 1 hr followed by viral-infection measured after 2 days by MTS assay
    Cytotoxicity against chicken embryo fibroblasts infected with Influenza A virus (A/Chicken/Shandong/LY/08(H9N2)) preincubated for 1 hr followed by viral-infection measured after 2 days by MTS assay
    [PMID: 24262883]
    HEK293 IC50
    > 10000 μM
    Compound: oseltamivir carboxylate
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU1 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    > 10000 μM
    Compound: oseltamivir carboxylate
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU3 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    > 10000 μM
    Compound: oseltamivir carboxylate
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU4 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    > 6000 μM
    Compound: oseltamivir carboxylate
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    Inhibition of human NEU2 expressed in HEK293 cells by fluorometric high-performance liquid chromatography using 4MU-NeuAc substrate
    [PMID: 18694948]
    HEK293 IC50
    0.05 μM
    Compound: OSV
    Inhibition of Influenza A virus (A/California/04/2009 (H1N1) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    Inhibition of Influenza A virus (A/California/04/2009 (H1N1) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    [PMID: 31324565]
    HEK293 IC50
    0.1 μM
    Compound: OSV
    Inhibition of Influenza A virus (A/Babol/36/2005 (H3N2) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    Inhibition of Influenza A virus (A/Babol/36/2005 (H3N2) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    [PMID: 31324565]
    HEK293 IC50
    2.6 μM
    Compound: OSV
    Inhibition of Influenza A virus (A/Anhui/1/2005(H5N1)) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    Inhibition of Influenza A virus (A/Anhui/1/2005(H5N1)) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    [PMID: 31324565]
    HEK293 IC50
    441.3 μM
    Compound: OSV
    Inhibition of Influenza A virus (A/Shanghai/1/2013 (H7N9)) neuraminidase R294K mutant expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay1
    Inhibition of Influenza A virus (A/Shanghai/1/2013 (H7N9)) neuraminidase R294K mutant expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay1
    [PMID: 31324565]
    HEK293 IC50
    9.6 μM
    Compound: OSV
    Inhibition of Influenza A virus (A/Anhui/1/2013 (H7N9) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    Inhibition of Influenza A virus (A/Anhui/1/2013 (H7N9) neuraminidase expressed by HEK293 cells incubated for 1 hr using 4-MU-NANA substrate by spectrofluorometric assay
    [PMID: 31324565]
    HEK-293T CC50
    > 250 μM
    Compound: OSV
    Cytotoxicity against HEK293T cells assessed as decrease in cell viability after 24 hrs by MTT assay
    Cytotoxicity against HEK293T cells assessed as decrease in cell viability after 24 hrs by MTT assay
    [PMID: 26924568]
    HEK-293T IC50
    21.09 ng/mL
    Compound: Oseltamivir
    Inhibition of wild type H1N1 swine influenza virus neuraminidase activity expressed in HEK293T cells after 2 hrs by spectrofluorometry
    Inhibition of wild type H1N1 swine influenza virus neuraminidase activity expressed in HEK293T cells after 2 hrs by spectrofluorometry
    [PMID: 21123068]
    HEK-293T IC50
    5.13 μg/mL
    Compound: Oseltamivir
    Inhibition of oseltamivir-resistant H1N1 swine influenza virus neuraminidase H274Y mutant activity expressed in HEK293T cells after 2 hrs by spectrofluorometry
    Inhibition of oseltamivir-resistant H1N1 swine influenza virus neuraminidase H274Y mutant activity expressed in HEK293T cells after 2 hrs by spectrofluorometry
    [PMID: 21123068]
    MDCK EC50
    < 0.01 μM
    Compound: OSV-C
    Antiviral activity against Influenza A virus (A/Hong kong/8/1968(H2N3)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    Antiviral activity against Influenza A virus (A/Hong kong/8/1968(H2N3)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    [PMID: 23419738]
    MDCK EC50
    > 10 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus A/Georgia/20/2006 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    Antiviral activity against Influenza A virus A/Georgia/20/2006 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    [PMID: 25019388]
    MDCK EC50
    > 10 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus A/Texas/48/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    Antiviral activity against Influenza A virus A/Texas/48/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    [PMID: 25019388]
    MDCK EC50
    > 10 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus A/Puerto Rico/8/34 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    Antiviral activity against Influenza A virus A/Puerto Rico/8/34 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    [PMID: 25019388]
    MDCK EC50
    > 10 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus A/California/07/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    Antiviral activity against Influenza A virus A/California/07/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    [PMID: 25019388]
    MDCK EC50
    > 10 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus A/Mexico/4108/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    Antiviral activity against Influenza A virus A/Mexico/4108/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    [PMID: 25019388]
    MDCK EC50
    > 100 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay
    [PMID: 20971531]
    MDCK CC50
    > 100 μg/mL
    Compound: Oseltamivir carboxylate
    Cytotoxicity against MDCK cells assessed as cell viability by MTS assay
    Cytotoxicity against MDCK cells assessed as cell viability by MTS assay
    [PMID: 20971531]
    MDCK CC50
    > 100 μg/mL
    Compound: Oseltamivir carboxylate
    Cytotoxicity against MDCK cells by MTS assay
    Cytotoxicity against MDCK cells by MTS assay
    [PMID: 20724039]
    MDCK EC50
    > 100 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE
    [PMID: 20971531]
    MDCK CC50
    > 100 μM
    Compound: OSV
    Cytotoxicity against MDCK cells after 40 hrs by Celltiter-Glo assay
    Cytotoxicity against MDCK cells after 40 hrs by Celltiter-Glo assay
    [PMID: 26686929]
    MDCK CC50
    > 100 μM
    Compound: Oseltamivir
    Cytotoxicity in MDCK cells assessed as reduction in cell viability incubated for 72 hrs by cell-titer-Glo cell viability assay
    Cytotoxicity in MDCK cells assessed as reduction in cell viability incubated for 72 hrs by cell-titer-Glo cell viability assay
    [PMID: 30192544]
    MDCK CC50
    > 100 μM
    Compound: Oseltamivir carboxylate
    Cytotoxicity against MDCK cells after 72 hrs by MTS assay
    Cytotoxicity against MDCK cells after 72 hrs by MTS assay
    [PMID: 25752526]
    MDCK CC50
    > 100 μM
    Compound: OSC
    Cytotoxicity against MDCK cells by MTS assay
    Cytotoxicity against MDCK cells by MTS assay
    [PMID: 19281225]
    MDCK CC50
    > 100 μM
    Compound: oseltamivir carboxylate
    Cytotoxicity against MDCK cells assessed as decrease in cell viability by MTS assay
    Cytotoxicity against MDCK cells assessed as decrease in cell viability by MTS assay
    [PMID: 18778942]
    MDCK CC50
    > 100 μM
    Compound: OSV-C
    Cytotoxicity against MDCK cells after 3 days by fluorescein diacetate-based fluorimetric analysis
    Cytotoxicity against MDCK cells after 3 days by fluorescein diacetate-based fluorimetric analysis
    [PMID: 23419738]
    MDCK CC50
    > 100 μM
    Compound: Oseltamivir
    Cytotoxicity against MDCK cells after 72 hrs by crystal violet staining based spectrophotometric method
    Cytotoxicity against MDCK cells after 72 hrs by crystal violet staining based spectrophotometric method
    [PMID: 29541360]
    MDCK CC50
    > 100 μM
    Compound: Oseltamivir carboxylate
    Cytotoxicity against MDCK cells assessed as cell viability by colorimetric formazan-based MTS assay
    Cytotoxicity against MDCK cells assessed as cell viability by colorimetric formazan-based MTS assay
    [PMID: 22459876]
    MDCK CC50
    > 1000 nM
    Compound: 2; (OC)
    Cytotoxicity against MDCK cells after 72 hrs by Alamar-Blue assay
    Cytotoxicity against MDCK cells after 72 hrs by Alamar-Blue assay
    [PMID: 28385598]
    MDCK EC50
    > 20 μM
    Compound: oseltamivir
    Antiviral activity against oseltamivir/amantadine-resistant Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as protection against virus-induced cytocidal effect after 48 hrs by CellTiter-Glo assay
    Antiviral activity against oseltamivir/amantadine-resistant Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as protection against virus-induced cytocidal effect after 48 hrs by CellTiter-Glo assay
    [PMID: 25815159]
    MDCK CC50
    > 200 μM
    Compound: OSV
    Cytotoxicity against MDCK cells after 40 hrs by CellTiter-Glo assay
    Cytotoxicity against MDCK cells after 40 hrs by CellTiter-Glo assay
    [PMID: 30554131]
    MDCK CC50
    > 200 μM
    Compound: OSV
    Cytotoxicity against MDCK cells assessed as cell growth after 40 hrs by CellTiter-Glo assay
    Cytotoxicity against MDCK cells assessed as cell growth after 40 hrs by CellTiter-Glo assay
    [PMID: 25681711]
    MDCK EC50
    > 200 μM
    Compound: OSV
    Antiviral activity against amantadine-resistant influenza A virus HN/1222 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    Antiviral activity against amantadine-resistant influenza A virus HN/1222 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    [PMID: 26686929]
    MDCK CC50
    > 250 μM
    Compound: OSV
    Cytotoxicity against MDCK cells assessed as decrease in cell viability after 48 hrs by MTT assay
    Cytotoxicity against MDCK cells assessed as decrease in cell viability after 48 hrs by MTT assay
    [PMID: 26924568]
    MDCK CC50
    > 300 μM
    Compound: Oseltamivir
    Cytotoxicity against MDCK cells after 48 hrs by MTT assay
    Cytotoxicity against MDCK cells after 48 hrs by MTT assay
    [PMID: 19729316]
    MDCK IC50
    > 500 μg/mL
    Compound: Oseltamivir
    Antiviral activity against oseltamivir-resistant Influenza A virus (A/Tianjinjinnan/15/2009/H1N1) infected in MDCK cells assessed as inhibition of virus-induced cytopathicity after 40 hrs
    Antiviral activity against oseltamivir-resistant Influenza A virus (A/Tianjinjinnan/15/2009/H1N1) infected in MDCK cells assessed as inhibition of virus-induced cytopathicity after 40 hrs
    [PMID: 24583605]
    MDCK CC50
    > 500 μM
    Compound: OSV
    Cytotoxicity against MDCK cells assessed as decrease in cell viability after 40 hrs by CellTiter-Glo assay
    Cytotoxicity against MDCK cells assessed as decrease in cell viability after 40 hrs by CellTiter-Glo assay
    10.1039/C6MD00292G
    MDCK CC50
    > 500 μM
    Compound: 1
    Cytotoxicity against MDCK cells infected with Influenza A virus (A/WSN/33(H1N1)) after 72 hrs by CellTiter-Glo assay
    Cytotoxicity against MDCK cells infected with Influenza A virus (A/WSN/33(H1N1)) after 72 hrs by CellTiter-Glo assay
    [PMID: 29141777]
    MDCK EC50
    > 80 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay
    [PMID: 25147619]
    MDCK CC50
    > 80 μM
    Compound: Oseltamivir
    Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo assay
    Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo assay
    [PMID: 25147619]
    MDCK EC50
    > 80 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus H3N2 VR1679 infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay
    Antiviral activity against Influenza A virus H3N2 VR1679 infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay
    [PMID: 25147619]
    MDCK CC50
    > 800 μM
    Compound: OSC
    Cytotoxicity against dog MDCK cells assessed as decrease in cell viability by CCK8 assay
    Cytotoxicity against dog MDCK cells assessed as decrease in cell viability by CCK8 assay
    [PMID: 34411893]
    MDCK EC50
    > 83.24 μM
    Compound: OSV
    Antiviral activity against influenza B virus B/SZ/155 infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    Antiviral activity against influenza B virus B/SZ/155 infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    [PMID: 26686929]
    MDCK IC50
    0.001 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/California/07/2009 (H1N1) infected in MDCK cells assessed as reduction in virus plaque formation after 5 days
    Antiviral activity against Influenza A virus A/California/07/2009 (H1N1) infected in MDCK cells assessed as reduction in virus plaque formation after 5 days
    [PMID: 24374271]
    MDCK IC50
    0.001 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/Hong Kong/8/1968 (H3N2) infected in MDCK cells assessed as reduction in virus plaque formation after 5 days
    Antiviral activity against Influenza A virus A/Hong Kong/8/1968 (H3N2) infected in MDCK cells assessed as reduction in virus plaque formation after 5 days
    [PMID: 24374271]
    MDCK EC50
    0.0014 μM
    Compound: oseltamivir carboxylate
    Inhibition of viral replication of influenza A virus (A/Vietnam/1203/2004 (H5N1)) and influenza A virus (A/Ann Arbor/6/60(H2N2)) hybrid virus in MDCK cells by neutral red uptake assay
    Inhibition of viral replication of influenza A virus (A/Vietnam/1203/2004 (H5N1)) and influenza A virus (A/Ann Arbor/6/60(H2N2)) hybrid virus in MDCK cells by neutral red uptake assay
    [PMID: 17194832]
    MDCK IC50
    0.004 μM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Hong Kong/1/1968(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
    Antiviral activity against Influenza A virus (A/Hong Kong/1/1968(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
    [PMID: 24313801]
    MDCK IC50
    0.015 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as protection against virus-induced cytopathic effect after 48 hrs by crystal violet staining
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as protection against virus-induced cytopathic effect after 48 hrs by crystal violet staining
    [PMID: 26316467]
    MDCK EC50
    0.015 μM
    Compound: OSV
    Antiviral activity against Influenza A virus PR/8/34 infected in MDCK cells assessed as inhibition of plaque formation treated for 1 hr measured after 2 days by toluidine blue staining-based assay
    Antiviral activity against Influenza A virus PR/8/34 infected in MDCK cells assessed as inhibition of plaque formation treated for 1 hr measured after 2 days by toluidine blue staining-based assay
    [PMID: 26924568]
    MDCK EC50
    0.02 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against influenza A virus H3N3 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay
    Antiviral activity against influenza A virus H3N3 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay
    [PMID: 23811093]
    MDCK IC50
    0.025 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as protection against virus-induced cytopathic effect after 48 hrs by crystal violet staining
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as protection against virus-induced cytopathic effect after 48 hrs by crystal violet staining
    [PMID: 26316467]
    MDCK IC50
    0.03 μM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus J/8178/09 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
    Antiviral activity against Influenza A virus J/8178/09 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
    [PMID: 24313801]
    MDCK EC50
    0.03 μM
    Compound: OST
    Antiviral activity against Influenza A virus infected in MDCK cells after 72 hrs by yield reduction assay
    Antiviral activity against Influenza A virus infected in MDCK cells after 72 hrs by yield reduction assay
    [PMID: 26643220]
    MDCK EC50
    0.032 μM
    Compound: oseltamivir carboxylate
    Inhibition of viral replication of influenza A virus (A/Hong Kong/213/03(H5N1)) and influenza A virus (A/Ann Arbor/6/60(H2N2)) hybrid virus in MDCK cells by neutral red uptake assay
    Inhibition of viral replication of influenza A virus (A/Hong Kong/213/03(H5N1)) and influenza A virus (A/Ann Arbor/6/60(H2N2)) hybrid virus in MDCK cells by neutral red uptake assay
    [PMID: 17194832]
    MDCK EC50
    0.05 μM
    Compound: OSV-C
    Antiviral activity against Influenza B virus (B/Taiwan/2/62) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    Antiviral activity against Influenza B virus (B/Taiwan/2/62) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    [PMID: 23419738]
    MDCK EC50
    0.05 μM
    Compound: OSV-C
    Antiviral activity against Influenza A virus (A/Taiwan/1/1986(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    Antiviral activity against Influenza A virus (A/Taiwan/1/1986(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    [PMID: 23419738]
    MDCK EC50
    0.06 μM
    Compound: OSV-C
    Antiviral activity against Influenza B virus (B/Panama/45/1990) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    Antiviral activity against Influenza B virus (B/Panama/45/1990) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    [PMID: 23419738]
    MDCK EC50
    0.09 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against influenza A virus H1N1 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay
    Antiviral activity against influenza A virus H1N1 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay
    [PMID: 23811093]
    MDCK EC50
    0.1 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as cell viability after 2 days by MTS assay
    Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as cell viability after 2 days by MTS assay
    [PMID: 20724039]
    MDCK EC50
    0.11 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    [PMID: 24900429]
    MDCK EC50
    0.11 μM
    Compound: Oseltamirvir-carboxylate
    Antiviral activity against Influenza A virus (A/duck/Minnesota/1525/1981(H5N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    Antiviral activity against Influenza A virus (A/duck/Minnesota/1525/1981(H5N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    [PMID: 23811085]
    MDCK EC50
    0.14 μM
    Compound: oseltamivir carboxylate
    Inhibition of influenza A virus (A/duck/Minnesota/1525/1981 (H5N1)) replication in MDCK cells by neutral red uptake assay
    Inhibition of influenza A virus (A/duck/Minnesota/1525/1981 (H5N1)) replication in MDCK cells by neutral red uptake assay
    [PMID: 17194832]
    MDCK IC50
    0.16 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Washington/01/2007 (H3N2)) measured in MDCK cells by NA inhibition assay
    Antiviral activity against Influenza A virus (A/Washington/01/2007 (H3N2)) measured in MDCK cells by NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    0.16 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus A/New York/18/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    Antiviral activity against Influenza A virus A/New York/18/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay
    [PMID: 25019388]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 250 nM of oseltamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 250 nM of oseltamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 250 nM of zanamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 250 nM of zanamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 625 nM of zanamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 625 nM of zanamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 25 nM of oseltamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 25 nM of oseltamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 50 nM of oseltamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 50 nM of oseltamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 1 nM of oseltamivir after 1 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 1 nM of oseltamivir after 1 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 5 nM of zanamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 5 nM of zanamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 25 nM of zanamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 25 nM of zanamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 50 nM of zanamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 50 nM of zanamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 1250 nM of compound after 6 passage after 48 hrs by hemagglutininin tit
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 1250 nM of compound after 6 passage after 48 hrs by hemagglutininin tit
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 1 nM of compound after 1 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 1 nM of compound after 1 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 5 nM of zanamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 5 nM of zanamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 25 nM of zanamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 25 nM of zanamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring wild type neuraminidase infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam/12
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring wild type neuraminidase infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam/12
    [PMID: 19651908]
    MDCK EC50
    0.2 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days
    Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days
    [PMID: 20724039]
    MDCK EC50
    0.28 μM
    Compound: oseltamivir carboxylate
    Inhibition of Influenza A virus (A/gull/Pennsylvania/4175/83 (H5N1)) replication in MDCK cells by neutral red uptake assay
    Inhibition of Influenza A virus (A/gull/Pennsylvania/4175/83 (H5N1)) replication in MDCK cells by neutral red uptake assay
    [PMID: 17194832]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 1 nM of oseltamivir after 1 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 1 nM of oseltamivir after 1 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 5 nM of oseltamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 5 nM of oseltamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 5 nM of oseltamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 5 nM of oseltamivir after 2 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 25 nM of oseltamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 25 nM of oseltamivir after 3 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 125 nM of oseltamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 125 nM of oseltamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 1 nM of zanamivir after 1 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) infected in MDCK cells selected at 1 nM of zanamivir after 1 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 125 nM of zanamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 125 nM of zanamivir after 4 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 3,125 nM of zanamivir after 6 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 3,125 nM of zanamivir after 6 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.3 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells selected at 16 uM of zanamivir after 7 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells selected at 16 uM of zanamivir after 7 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.4 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 625 nM of oseltamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) infected in MDCK cells selected at 625 nM of oseltamivir after 5 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.4 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 6250 nM of compound after 7 passage after 48 hrs by hemagglutininin tit
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 6250 nM of compound after 7 passage after 48 hrs by hemagglutininin tit
    [PMID: 19651908]
    MDCK IC50
    0.4 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222M mutant infected in MDCK cells selected at 3,125 nM of oseltamivir after 6 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222M mutant infected in MDCK cells selected at 3,125 nM of oseltamivir after 6 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    0.44 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Washington/01/2007 (H3N2)) measured in MDCK cells by fluorescent NA inhibition assay
    Antiviral activity against Influenza A virus (A/Washington/01/2007 (H3N2)) measured in MDCK cells by fluorescent NA inhibition assay
    [PMID: 20194700]
    MDCK IC50
    0.7 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam
    [PMID: 19651908]
    MDCK EC50
    0.726 ng/mL
    Compound: oseltamivir carboxylate
    Antiviral activity against influenza virus (A/Sidney/5/97 (H3N2)) R292 infected in MDCK cells expressing human ST6Gal 1 gene in hollow fiber model system assessed as decrease in viral replication after 48 to 72 hrs by plaque assay
    Antiviral activity against influenza virus (A/Sidney/5/97 (H3N2)) R292 infected in MDCK cells expressing human ST6Gal 1 gene in hollow fiber model system assessed as decrease in viral replication after 48 to 72 hrs by plaque assay
    [PMID: 19364864]
    MDCK IC50
    0.8 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring wild type neuraminidase infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring wild type neuraminidase infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK EC50
    0.82 μM
    Compound: OSV
    Antiviral activity against oseltamivir-resistant influenza A virus LN/1109 (H1N1) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    Antiviral activity against oseltamivir-resistant influenza A virus LN/1109 (H1N1) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    [PMID: 26686929]
    MDCK EC50
    0.82 μM
    Compound: OSV-C
    Antiviral activity against Influenza B virus (B/Lee/40) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    Antiviral activity against Influenza B virus (B/Lee/40) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    [PMID: 23419738]
    MDCK IC50
    0.9 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells selected at 78 uM of zanamivir after 8 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells selected at 78 uM of zanamivir after 8 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    1 μg/mL
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/hanfang/359/95(H3N2) infected in MDCK cells assessed as inhibition of virus-induced cytopathicity after 40 hrs
    Antiviral activity against Influenza A virus A/hanfang/359/95(H3N2) infected in MDCK cells assessed as inhibition of virus-induced cytopathicity after 40 hrs
    [PMID: 24583605]
    MDCK EC50
    1.1 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against influenza B virus infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay
    Antiviral activity against influenza B virus infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay
    [PMID: 23811093]
    MDCK IC50
    1.5 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring wild type neuraminidase infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Caledo
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring wild type neuraminidase infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Caledo
    [PMID: 19651908]
    MDCK EC50
    1.5 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against influenza B virus infected in MDCK cells assessed as cell viability after 2 days by MTS assay
    Antiviral activity against influenza B virus infected in MDCK cells assessed as cell viability after 2 days by MTS assay
    [PMID: 20724039]
    MDCK EC50
    1.5 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against amantadine-sensitive Influenza A virus (A/Ned/378/05 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    Antiviral activity against amantadine-sensitive Influenza A virus (A/Ned/378/05 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    [PMID: 24900429]
    MDCK IC50
    10 μM
    Compound: OSV
    Antiviral activity against Influenza A virus A/WSN/1933 (H1N1) infected in MDCK cells assessed as protection against virus induced cytopathic effect after 40 hrs by Celltiter-Glo assay
    Antiviral activity against Influenza A virus A/WSN/1933 (H1N1) infected in MDCK cells assessed as protection against virus induced cytopathic effect after 40 hrs by Celltiter-Glo assay
    [PMID: 26686929]
    MDCK EC50
    10.23 ng/mL
    Compound: oseltamivir carboxylate
    Antiviral activity against influenza virus (A/Sidney/5/97 (H3N2)) R292 infected in MDCK cells expressing human ST6Gal 1 gene after 48 to 72 hrs by plaque assay
    Antiviral activity against influenza virus (A/Sidney/5/97 (H3N2)) R292 infected in MDCK cells expressing human ST6Gal 1 gene after 48 to 72 hrs by plaque assay
    [PMID: 19364864]
    MDCK EC50
    11.6 μM
    Compound: OSC
    Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method
    Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method
    [PMID: 19281225]
    MDCK IC50
    11.9 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222M mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Cal
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222M mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Cal
    [PMID: 19651908]
    MDCK IC50
    1178.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Cal
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Cal
    [PMID: 19651908]
    MDCK EC50
    12 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE
    Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE
    [PMID: 20971531]
    MDCK EC50
    12 μM
    Compound: OSC
    Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method
    Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method
    [PMID: 19281225]
    MDCK EC50
    13.63 μM
    Compound: OSV-C
    Antiviral activity against Influenza B virus (B/Brisbane/60/2008) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    Antiviral activity against Influenza B virus (B/Brisbane/60/2008) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis
    [PMID: 23419738]
    MDCK IC50
    1390 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M and H274Y mutant infected in MDCK cells selected at 1950 uM of oseltamivir after 10 passage after 48 hrs by hem
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M and H274Y mutant infected in MDCK cells selected at 1950 uM of oseltamivir after 10 passage after 48 hrs by hem
    [PMID: 19651908]
    MDCK IC50
    145.2 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells selected at 6250 nM of oseltamivir after 7 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells selected at 6250 nM of oseltamivir after 7 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    1469.7 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222V and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222V and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK EC50
    15 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay
    Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay
    [PMID: 25752526]
    MDCK IC50
    15 μM
    Compound: OSV
    Antiviral activity against influenza A virus A/WSN/33 (H1N1) infected in MDCK cells assessed as inhibition of plaque formation after 3 days at 37 degC
    Antiviral activity against influenza A virus A/WSN/33 (H1N1) infected in MDCK cells assessed as inhibition of plaque formation after 3 days at 37 degC
    [PMID: 25681711]
    MDCK IC50
    1502.8 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222V and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222V and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus
    [PMID: 19651908]
    MDCK IC50
    152.8 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin A45S, K156E, P210Q, L313F mutant gene and neuraminidase H274Y mutant infected in MDCK cells selected at 781 uM of oseltamivir after 10 passage after 48 hrs
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin A45S, K156E, P210Q, L313F mutant gene and neuraminidase H274Y mutant infected in MDCK cells selected at 781 uM of oseltamivir after 10 passage after 48 hrs
    [PMID: 19651908]
    MDCK IC50
    154 nM
    Compound: oseltamivir
    Antiviral activity against sInfluenza A virus (A/Texas/12/2007 (H3N2)) with neuraminidase E119(V/I) mutant isolated from immunocompromized patient measured after X/CD3 additional passages in MDCK cells by fluorescent NA inhibition assay
    Antiviral activity against sInfluenza A virus (A/Texas/12/2007 (H3N2)) with neuraminidase E119(V/I) mutant isolated from immunocompromized patient measured after X/CD3 additional passages in MDCK cells by fluorescent NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    16 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against amantadine-sensitive Influenza A virus (A/FM/1/47 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    Antiviral activity against amantadine-sensitive Influenza A virus (A/FM/1/47 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    [PMID: 24900429]
    MDCK IC50
    16.9 nM
    Compound: Tamiflu
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs by crystal violet staining-based microplate reader analysis
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs by crystal violet staining-based microplate reader analysis
    [PMID: 26697718]
    MDCK CC50
    160.3 μM
    Compound: Oseltamivir
    Cytotoxicity against MDCK cells after 48 hrs by MTT assay
    Cytotoxicity against MDCK cells after 48 hrs by MTT assay
    [PMID: 30108810]
    MDCK IC50
    177 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells selected at 31 uM of oseltamivir after 8 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells selected at 31 uM of oseltamivir after 8 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    18.2 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M mutant infected in MDCK cells selected at 16 uM of oseltamivir after 7 passage after 48 hrs by hemagglutininin
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M mutant infected in MDCK cells selected at 16 uM of oseltamivir after 7 passage after 48 hrs by hemagglutininin
    [PMID: 19651908]
    MDCK IC50
    18.5 nM
    Compound: Tamiflu
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs by crystal violet staining-based microplate reader analysis
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs by crystal violet staining-based microplate reader analysis
    [PMID: 26697718]
    MDCK EC50
    18.99 μM
    Compound: Oseltamirvir-carboxylate
    Antiviral activity against Influenza A virus (A/California/07/2009(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    Antiviral activity against Influenza A virus (A/California/07/2009(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    [PMID: 23811085]
    MDCK IC50
    180.3 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin A45S, K156E, P210Q, L313F mutant gene and neuraminidase H274Y mutant infected in MDCK cells selected at 31 uM of oseltamivir after 9 passage after 48 hrs b
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin A45S, K156E, P210Q, L313F mutant gene and neuraminidase H274Y mutant infected in MDCK cells selected at 31 uM of oseltamivir after 9 passage after 48 hrs b
    [PMID: 19651908]
    MDCK IC50
    191.6 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M and H274Y mutant infected in MDCK cells selected at 390 uM of oseltamivir after 9 passage after 48 hrs by hemag
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M and H274Y mutant infected in MDCK cells selected at 390 uM of oseltamivir after 9 passage after 48 hrs by hemag
    [PMID: 19651908]
    MDCK IC50
    194.1 nM
    Compound: B
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) in MDCK cells assessed as reduction in plaque number by plaque reduction assay
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) in MDCK cells assessed as reduction in plaque number by plaque reduction assay
    [PMID: 20014777]
    MDCK EC50
    2 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against influenza B virus infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days
    Antiviral activity against influenza B virus infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days
    [PMID: 20724039]
    MDCK EC50
    2 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis
    [PMID: 25752526]
    MDCK IC50
    2.1 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 31 uM of compound after 8 passage after 48 hrs by hemagglutininin titer
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 31 uM of compound after 8 passage after 48 hrs by hemagglutininin titer
    [PMID: 19651908]
    MDCK EC50
    2.14 μM
    Compound: Oseltamivir
    Antiviral activity against influenza A virus H3N2/95-359 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by Reed-Muench method
    Antiviral activity against influenza A virus H3N2/95-359 infected in MDCK cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by Reed-Muench method
    [PMID: 32378892]
    MDCK EC50
    2.24 μM
    Compound: oseltamivir
    Cytotoxicity against MDCK cells after 48 hrs by neutral red dye assay
    Cytotoxicity against MDCK cells after 48 hrs by neutral red dye assay
    [PMID: 20886838]
    MDCK EC50
    2.3 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay
    Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay
    [PMID: 25752526]
    MDCK IC50
    2.3 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G and E119G mutant infected in MDCK cells selected at 31 uM of zanamivir after 9 passage after 48 hrs by hemaggluti
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G and E119G mutant infected in MDCK cells selected at 31 uM of zanamivir after 9 passage after 48 hrs by hemaggluti
    [PMID: 19651908]
    MDCK EC50
    2.6 μM
    Compound: OSC
    Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method
    Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method
    [PMID: 19281225]
    MDCK IC50
    2.81 μg/mL
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/FM/1/1947(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathicity after 40 hrs
    Antiviral activity against Influenza A virus (A/FM/1/1947(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathicity after 40 hrs
    [PMID: 24583605]
    MDCK EC50
    2.9 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay
    [PMID: 25752526]
    MDCK EC50
    20 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis
    Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis
    [PMID: 25752526]
    MDCK EC50
    21 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against amantadine-resistant Influenza A virus (A/PR/8/34 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    Antiviral activity against amantadine-resistant Influenza A virus (A/PR/8/34 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    [PMID: 24900429]
    MDCK EC50
    21.8 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay
    [PMID: 32214762]
    MDCK IC50
    245.5 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 1 with neuraminidase E119V mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by fluorescent NA inhibition assay
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 1 with neuraminidase E119V mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by fluorescent NA inhibition assay
    [PMID: 20194700]
    MDCK IC50
    25.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells selected at 1250 nM of oseltamivir after 6 passage after 48 hrs by hemagglutininin titer assay
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells selected at 1250 nM of oseltamivir after 6 passage after 48 hrs by hemagglutininin titer assay
    [PMID: 19651908]
    MDCK IC50
    27.8 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222M mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222M mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK EC50
    28 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay
    Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay
    [PMID: 20971531]
    MDCK EC50
    29 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/HK/7/87 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    Antiviral activity against Influenza A virus (A/HK/7/87 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    [PMID: 24900429]
    MDCK IC50
    3 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 781 uM of zanamivir after 10 passage after 48 hrs by hemagglutininin ti
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring hemagglutininin K234N mutant gene and neuraminidase D198G mutant infected in MDCK cells selected at 781 uM of zanamivir after 10 passage after 48 hrs by hemagglutininin ti
    [PMID: 19651908]
    MDCK EC50
    3 μM
    Compound: oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/Ishikawa/7/82 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    Antiviral activity against Influenza A virus (A/Ishikawa/7/82 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis
    [PMID: 24900429]
    MDCK EC50
    3.06 μM
    Compound: Oseltamirvir-carboxylate
    Antiviral activity against Influenza A virus (A/Perth/16/2009(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    Antiviral activity against Influenza A virus (A/Perth/16/2009(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    [PMID: 23811085]
    MDCK IC50
    3.1 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase E119G mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK EC50
    3.52 μM
    Compound: Oseltamirvir-carboxylate
    Antiviral activity against Influenza B virus (B/Florida/4/2006) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    Antiviral activity against Influenza B virus (B/Florida/4/2006) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect by cell-based neutral red uptake assay
    [PMID: 23811085]
    MDCK EC50
    3.8 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as cell viability after 2 days by MTS assay
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as cell viability after 2 days by MTS assay
    [PMID: 20724039]
    MDCK CC50
    300 μg/mL
    Compound: Oseltamivir
    Cytotoxicity against MDCK cells assessed as cell death incubated for 48 hrs by MTT assay
    Cytotoxicity against MDCK cells assessed as cell death incubated for 48 hrs by MTT assay
    [PMID: 27720324]
    MDCK IC50
    3009.1 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222M and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222M and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus
    [PMID: 19651908]
    MDCK CC50
    321 μM
    Compound: OST
    Cytotoxicity against MDCK cells after 72 hrs by XTT assay
    Cytotoxicity against MDCK cells after 72 hrs by XTT assay
    [PMID: 26643220]
    MDCK IC50
    33.25 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 2 with neuraminidase E119I mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by NA inhibition assay
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 2 with neuraminidase E119I mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    39.1 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Unidentified Influenza A virus (H1N1) infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay
    Antiviral activity against Unidentified Influenza A virus (H1N1) infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay
    [PMID: 32214762]
    MDCK EC50
    4 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days
    [PMID: 20724039]
    MDCK EC50
    4 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days
    [PMID: 22459876]
    MDCK EC50
    4 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis
    Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis
    [PMID: 25752526]
    MDCK IC50
    4.5 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222V mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Cal
    Antiviral activity against Influenza A virus A/New Caledonia/20/99 (H1N1)) harboring neuraminidase I222V mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus A/New Cal
    [PMID: 19651908]
    MDCK EC50
    400 μM
    Compound: OSC
    Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method
    Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method
    [PMID: 19281225]
    MDCK IC50
    430.59 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 2 with neuraminidase E119I mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by fluorescent NA inhibition assay
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 2 with neuraminidase E119I mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by fluorescent NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    45 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days
    [PMID: 22459876]
    MDCK IC50
    46.1 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect incubated for 48 hrs by crystal violet staining based assay
    Antiviral activity against Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect incubated for 48 hrs by crystal violet staining based assay
    [PMID: 32520560]
    MDCK IC50
    46.5 μM
    Compound: OSV
    Antiviral activity against influenza A virus A/WSN/33 (H1N1) infected in MDCK cells assessed as reduction in cytopathic effect after 48 hrs by plaque assay
    Antiviral activity against influenza A virus A/WSN/33 (H1N1) infected in MDCK cells assessed as reduction in cytopathic effect after 48 hrs by plaque assay
    [PMID: 25681711]
    MDCK IC50
    498.9 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam
    [PMID: 19651908]
    MDCK IC50
    5.3 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222V mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222V mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK IC50
    5.49 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 1 with neuraminidase E119V mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by NA inhibition assay
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) clone 1 with neuraminidase E119V mutant isolated from immunocompromized patient measured after X/CD4 additional passages in MDCK cells by NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    5.6 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay
    Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay
    [PMID: 22459876]
    MDCK EC50
    5.7 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H3N2 infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay
    Antiviral activity against Influenza A virus H3N2 infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay
    [PMID: 32214762]
    MDCK IC50
    5.7 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K205EQ mutant gene and neuraminidase E119G mutant infected in MDCK cells selected at 1950 uM of zanamivir after 10 passage after 48 hrs by hemagglutinini
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K205EQ mutant gene and neuraminidase E119G mutant infected in MDCK cells selected at 1950 uM of zanamivir after 10 passage after 48 hrs by hemagglutinini
    [PMID: 19651908]
    MDCK EC50
    583 nM
    Compound: Oseltamivir carboxylate, Tamiflu
    Antiviral activity against Influenza B virus (B/Harbin/07/94) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by MTT assay
    Antiviral activity against Influenza B virus (B/Harbin/07/94) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by MTT assay
    10.1039/C2MD20300F
    MDCK IC50
    6.2 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K205EQ mutant gene and neuraminidase E119G mutant infected in MDCK cells selected at 390 uM of zanamivir after 9 passage after 48 hrs by hemagglutininin
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K205EQ mutant gene and neuraminidase E119G mutant infected in MDCK cells selected at 390 uM of zanamivir after 9 passage after 48 hrs by hemagglutininin
    [PMID: 19651908]
    MDCK IC50
    6.4 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase D198G mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam
    Antiviral activity against Influenza A virus (A/Vietnam/1203/2004 (H5N1)) harboring neuraminidase D198G mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose to IC50 for Influenza A virus (A/Vietnam
    [PMID: 19651908]
    MDCK EC50
    6.9 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay
    Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay
    [PMID: 20971531]
    MDCK IC50
    6126 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222M and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase I222M and H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK IC50
    695.6 nM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring neuraminidase H274Y mutant infected in MDCK cells assessed as inhibition of viral-induced cytopathogenicity after 4 days post dose
    [PMID: 19651908]
    MDCK EC50
    77.6 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay
    Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay
    [PMID: 22459876]
    MDCK CC50
    7865.4 μM
    Compound: Oseltamivir
    Cytotoxicity against MDCK cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against MDCK cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    [PMID: 34425312]
    MDCK IC50
    8.06 nM
    Compound: oseltamivir
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) with neuraminidase E119(V/I) mutant isolated from immunocompromized patient measured after X/CD3 additional passages in MDCK cells by NA inhibition assay
    Antiviral activity against Influenza A virus (A/Texas/12/2007 (H3N2)) with neuraminidase E119(V/I) mutant isolated from immunocompromized patient measured after X/CD3 additional passages in MDCK cells by NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    800 μM
    Compound: OSC
    Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method
    Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method
    [PMID: 19281225]
    MDCK IC50
    89.2 μM
    Compound: Oseltamivir
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M and H274Y mutant infected in MDCK cells selected at 78 uM of oseltamivir after 8 passage after 48 hrs by hemagg
    Antiviral activity against Influenza A virus (A/Chicken/Laos/26/2006(H5N1)) harboring hemagglutininin K234Q mutant gene and neuraminidase I222M and H274Y mutant infected in MDCK cells selected at 78 uM of oseltamivir after 8 passage after 48 hrs by hemagg
    [PMID: 19651908]
    MDCK EC50
    9 μg/mL
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE
    Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE
    [PMID: 20971531]
    MDCK EC50
    9 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days
    [PMID: 22459876]
    MDCK IC50
    9.18 nM
    Compound: oseltamivir
    Antiviral activity against sInfluenza A virus (A/Texas/12/2007 (H3N2)) with neuraminidase E119V mutant isolated from immunocompromized patient measured after X/CD2 additional passages in MDCK cells by NA inhibition assay
    Antiviral activity against sInfluenza A virus (A/Texas/12/2007 (H3N2)) with neuraminidase E119V mutant isolated from immunocompromized patient measured after X/CD2 additional passages in MDCK cells by NA inhibition assay
    [PMID: 20194700]
    MDCK EC50
    9.7 μM
    Compound: Oseltamivir carboxylate
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay
    Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay
    [PMID: 22459876]
    MDCK EC50
    9.75 μM
    Compound: OSV
    Antiviral activity against amantadine-resistant influenza A virus JX/312 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    Antiviral activity against amantadine-resistant influenza A virus JX/312 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay
    [PMID: 26686929]
    MDCK EC50
    900 μM
    Compound: OSC
    Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method
    Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method
    [PMID: 19281225]
    MDCK EC50
    98 nM
    Compound: Oseltamivir carboxylate, Tamiflu
    Antiviral activity against Influenza A virus (A/Sydney/5/1997(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by MTT assay
    Antiviral activity against Influenza A virus (A/Sydney/5/1997(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by MTT assay
    10.1039/C2MD20300F
    Vero C1008 CC50
    > 100 μM
    Compound: Oseltamivir
    Cytotoxicity against African green monkey Vero E6 cells assessed as cell growth inhibition incubated for 48 hrs
    Cytotoxicity against African green monkey Vero E6 cells assessed as cell growth inhibition incubated for 48 hrs
    [PMID: 32771797]
    In Vivo

    Oseltamivir (20 mg/kg/day, p.o., twice daily for 10 days) prevented death in the H1N1pdm virus infection in mice[2].
    Oseltamivir (10 mg/kg/day, oral gavage, 5 days) together with Amantadine (HY-B0402) (15 or 30 mg/kg) has greater protection efficiency against H5N1 influenza virus infection in mice than monotherapy[3].
    Oseltamivir is also used together with Ribavirin (HY-B0434), or Favipiravir (HY-14768), or Peramivir (HY-17015A) to prevent influenza virus infection in mice[4][5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    312.40

    Formula

    C16H28N2O4

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OCC)C1=C[C@H]([C@@H]([C@H](C1)N)NC(C)=O)OC(CC)CC

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (320.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2010 mL 16.0051 mL 32.0102 mL
    5 mM 0.6402 mL 3.2010 mL 6.4020 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.94%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.2010 mL 16.0051 mL 32.0102 mL 80.0256 mL
    5 mM 0.6402 mL 3.2010 mL 6.4020 mL 16.0051 mL
    10 mM 0.3201 mL 1.6005 mL 3.2010 mL 8.0026 mL
    15 mM 0.2134 mL 1.0670 mL 2.1340 mL 5.3350 mL
    20 mM 0.1601 mL 0.8003 mL 1.6005 mL 4.0013 mL
    25 mM 0.1280 mL 0.6402 mL 1.2804 mL 3.2010 mL
    30 mM 0.1067 mL 0.5335 mL 1.0670 mL 2.6675 mL
    40 mM 0.0800 mL 0.4001 mL 0.8003 mL 2.0006 mL
    50 mM 0.0640 mL 0.3201 mL 0.6402 mL 1.6005 mL
    60 mM 0.0534 mL 0.2668 mL 0.5335 mL 1.3338 mL
    80 mM 0.0400 mL 0.2001 mL 0.4001 mL 1.0003 mL
    100 mM 0.0320 mL 0.1601 mL 0.3201 mL 0.8003 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Requested Quantity *

    Applicant Name *

     

    Salutation

    Email Address *

     

    Phone Number *

    Department

     

    Organization Name *

    City

    State

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Oseltamivir
    Cat. No.:
    HY-13317
    Quantity:
    MCE Japan Authorized Agent: