Amantadine (Synonyms: 1-Adamantanamine; 1-Aminoadamantane)

Name: Amantadine
Brand: MedChemExpress
SKU: HY-B0402
Rating: 5.0 (4 reviews)

Cat. No.: HY-B0402 Purity: 99.90%: FAQs
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Amantadine (1-Adamantanamine) is an orally avtive and potent antiviral agent with activity against influenza A viruses. Amantadine inhibits several ion channels such as NMDA and M2, and also inhibits Coronavirus ion channels. Amantadine also has anti-orthopoxvirus and anticancer activity. Amantadine can be used for Parkinson's disease, postoperative cognitive dysfunction (POCD) and COVID-19 research.

For research use only. We do not sell to patients.

Amantadine Chemical Structure

CAS No. : 768-94-5

Size	Price	Stock	Quantity
500 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
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Based on 4 publication(s) in Google Scholar

Other Forms of Amantadine:

4 Publications Citing Use of MCE Amantadine

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

View All Bcl-2 Family Isoform Specific Products:

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

CDK2

Bcl-2

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Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	1.6 μM Compound: 1	Blockade of Influenza A virus (A/England/195/2009(H1N1)) full length M2 proton channel expressed in HEK cells after 3 mins by voltage clamp technique Blockade of Influenza A virus (A/England/195/2009(H1N1)) full length M2 proton channel expressed in HEK cells after 3 mins by voltage clamp technique	[PMID: 24793875]
HEK293	IC₅₀	3.525 μM Compound: 1	Inhibition of avian influenza virus H5N1 matrix 2 protein expressed in HEK293 cells assessed as cell viability after 24 hrs Inhibition of avian influenza virus H5N1 matrix 2 protein expressed in HEK293 cells assessed as cell viability after 24 hrs	[PMID: 20394375]
HEK293	IC₅₀	37.8 μM Compound: amantadine	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	8.8 μM Compound: 1	Inhibition of avian influenza virus (H5N1) matrix 2 protein expressed in HEK293 cells by path clamp electrophysiology method Inhibition of avian influenza virus (H5N1) matrix 2 protein expressed in HEK293 cells by path clamp electrophysiology method	[PMID: 20394375]
MDCK	EC₅₀	> 10 μM Compound: amantadine	Antiviral activity against Influenza A virus A/Georgia/20/2006 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay Antiviral activity against Influenza A virus A/Georgia/20/2006 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay	[PMID: 25019388]
MDCK	EC₅₀	> 10 μM Compound: amantadine	Antiviral activity against Influenza A virus A/Texas/48/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay Antiviral activity against Influenza A virus A/Texas/48/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay	[PMID: 25019388]
MDCK	EC₅₀	> 10 μM Compound: amantadine	Antiviral activity against Influenza A virus A/Puerto Rico/8/34 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay Antiviral activity against Influenza A virus A/Puerto Rico/8/34 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay	[PMID: 25019388]
MDCK	EC₅₀	> 10 μM Compound: amantadine	Antiviral activity against Influenza A virus A/California/07/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay Antiviral activity against Influenza A virus A/California/07/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay	[PMID: 25019388]
MDCK	EC₅₀	> 10 μM Compound: amantadine	Antiviral activity against Influenza A virus A/Mexico/4108/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay Antiviral activity against Influenza A virus A/Mexico/4108/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay	[PMID: 25019388]
MDCK	EC₅₀	> 10 μM Compound: amantadine	Antiviral activity against Influenza A virus A/New York/18/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay Antiviral activity against Influenza A virus A/New York/18/2009 infected in MDCK cells assessed as cell protection after 3 days by cytopathic effect assay	[PMID: 25019388]
MDCK	CC₅₀	> 100 μM Compound: AMD	Cytotoxicity against MDCK cells after 3 days by CCK-8 assay Cytotoxicity against MDCK cells after 3 days by CCK-8 assay	[PMID: 22332894]
MDCK	CC₅₀	> 100 μM Compound: Amantadine	Cytotoxicity against MDCK cells after 40 hrs by Celltiter-Glo assay Cytotoxicity against MDCK cells after 40 hrs by Celltiter-Glo assay	[PMID: 26686929]
MDCK	CC₅₀	> 100 μM Compound: Amantadine	Cytotoxicity against dog MDCK cells assessed as reduction in cell viability incubated for 3 days by coulter counter method Cytotoxicity against dog MDCK cells assessed as reduction in cell viability incubated for 3 days by coulter counter method	[PMID: 31586832]
MDCK	CC₅₀	> 100 μM Compound: amantadine	Cytotoxicity against dog MDCK cells assessed as cell viability after 72 hrs by MTS assay Cytotoxicity against dog MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 24900429]
MDCK	EC₅₀	> 100 μM Compound: amantadine	Antiviral activity against amantidine and rimantadine-resistant Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect by MTS assay Antiviral activity against amantidine and rimantadine-resistant Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect by MTS assay	[PMID: 24900429]
MDCK	CC₅₀	> 100 μM Compound: AMN	Cytotoxicity against MDCK cells by MTS assay Cytotoxicity against MDCK cells by MTS assay	[PMID: 19281225]
MDCK	EC₅₀	> 100 μM Compound: AMN	Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method	[PMID: 19281225]
MDCK	EC₅₀	> 100 μM Compound: AMN	Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method	[PMID: 19281225]
MDCK	CC₅₀	> 100 μM Compound: amantadin	Cytotoxicity against MDCK cells assessed as decrease in cell viability by MTS assay Cytotoxicity against MDCK cells assessed as decrease in cell viability by MTS assay	[PMID: 18778942]
MDCK	CC₅₀	> 100 μM Compound: AMD	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31559005]
MDCK	CC₅₀	> 100 μM Compound: AMT	Cytotoxicity against MDCK cells assessed as reduction in cell viability measured for 48 hrs by MTT assay Cytotoxicity against MDCK cells assessed as reduction in cell viability measured for 48 hrs by MTT assay	[PMID: 36764470]
MDCK	EC₅₀	> 100 μM Compound: AMT	Antiviral activity against Influenza B virus (B/Brisbane/60/2008) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis Antiviral activity against Influenza B virus (B/Brisbane/60/2008) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis	[PMID: 23419738]
MDCK	EC₅₀	> 100 μM Compound: AMT	Antiviral activity against Influenza B virus (B/Lee/40) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis Antiviral activity against Influenza B virus (B/Lee/40) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis	[PMID: 23419738]
MDCK	EC₅₀	> 100 μM Compound: AMT	Antiviral activity against Influenza B virus (B/Taiwan/2/62) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis Antiviral activity against Influenza B virus (B/Taiwan/2/62) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis	[PMID: 23419738]
MDCK	EC₅₀	> 100 μM Compound: AMT	Antiviral activity against Influenza B virus (B/Panama/45/1990) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis Antiviral activity against Influenza B virus (B/Panama/45/1990) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis	[PMID: 23419738]
MDCK	CC₅₀	> 100 μM Compound: AMT	Cytotoxicity against MDCK cells after 3 days by fluorescein diacetate-based fluorimetric analysis Cytotoxicity against MDCK cells after 3 days by fluorescein diacetate-based fluorimetric analysis	[PMID: 23419738]
MDCK	IC₅₀	> 100 μM Compound: amantadine	Antiviral activity against amantadine-sensitive Influenza A virus A/HK/68 (H3N2) expressing wild type M2 ion channel infected in MDCK cells assessed as protection against virus induced cytopathic effect after 48 hrs by microscopic analysis Antiviral activity against amantadine-sensitive Influenza A virus A/HK/68 (H3N2) expressing wild type M2 ion channel infected in MDCK cells assessed as protection against virus induced cytopathic effect after 48 hrs by microscopic analysis	[PMID: 26722757]
MDCK	CC₅₀	> 100 μM Compound: 1	Cytotoxicity against MDCK cells after 72 hrs by crystal violet staining based spectrophotometric method Cytotoxicity against MDCK cells after 72 hrs by crystal violet staining based spectrophotometric method	[PMID: 29541360]
MDCK	CC₅₀	> 100 μM Compound: 1	Cytotoxicity against MDCK cells measured after 72 hrs et staining-based microscopic analysis Cytotoxicity against MDCK cells measured after 72 hrs et staining-based microscopic analysis	[PMID: 28107633]
MDCK	EC₅₀	> 100 μM Compound: amantadine	Antiviral activity against amantidine and rimantadine-resistant Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against amantidine and rimantadine-resistant Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	EC₅₀	> 100 μM Compound: AMN	Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method Antiviral activity against influenza H1N1 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method	[PMID: 19281225]
MDCK	EC₅₀	> 100 μM Compound: AMN	Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method Antiviral activity against influenza B virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method	[PMID: 19281225]
MDCK	CC₅₀	> 1000 μg/mL Compound: Amantadin	Cytotoxicity against MDCK cells by MTS assay Cytotoxicity against MDCK cells by MTS assay	[PMID: 20724039]
MDCK	EC₅₀	> 20 μM Compound: Amantadine	Antiviral activity against oseltamivir/amantadine-resistant Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as protection against virus-induced cytocidal effect after 48 hrs by CellTiter-Glo assay Antiviral activity against oseltamivir/amantadine-resistant Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as protection against virus-induced cytocidal effect after 48 hrs by CellTiter-Glo assay	[PMID: 25815159]
MDCK	EC₅₀	> 20.51 μM Compound: Amantadine	Antiviral activity against amantadine-resistant influenza A virus HN/1222 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay Antiviral activity against amantadine-resistant influenza A virus HN/1222 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay	[PMID: 26686929]
MDCK	EC₅₀	> 200 μg/mL Compound: Amantadine	Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay	[PMID: 20971531]
MDCK	CC₅₀	> 200 μg/mL Compound: Amantadine	Cytotoxicity against MDCK cells assessed as cell viability by MTS assay Cytotoxicity against MDCK cells assessed as cell viability by MTS assay	[PMID: 20971531]
MDCK	EC₅₀	> 200 μg/mL Compound: Amantadine	Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE	[PMID: 20971531]
MDCK	CC₅₀	> 200 μM Compound: amantadine	Cytotoxicity against MDCK cells assessed as cell growth after 40 hrs by CellTiter-Glo assay Cytotoxicity against MDCK cells assessed as cell growth after 40 hrs by CellTiter-Glo assay	[PMID: 25681711]
MDCK	EC₅₀	> 200 μM Compound: Amantadine	Antiviral activity against oseltamivir-resistant influenza A virus LN/1109 (H1N1) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay Antiviral activity against oseltamivir-resistant influenza A virus LN/1109 (H1N1) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay	[PMID: 26686929]
MDCK	CC₅₀	> 200 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 4 days by MTS assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 4 days by MTS assay	[PMID: 31753804]
MDCK	EC₅₀	> 200 μM Compound: amantadine	Antiviral activity against influenza B virus infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay Antiviral activity against influenza B virus infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay	[PMID: 23811093]
MDCK	CC₅₀	> 200 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as cell viability measured after 4 days by formazan-based MTS assay Cytotoxicity against MDCK cells assessed as cell viability measured after 4 days by formazan-based MTS assay	[PMID: 27112451]
MDCK	EC₅₀	> 200 μM Compound: Amantadine	Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay Antiviral activity against Influenza B virus infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay	[PMID: 22459876]
MDCK	EC₅₀	> 200 μM Compound: Amantadine	Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days	[PMID: 22459876]
MDCK	CC₅₀	> 200 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as cell viability by colorimetric formazan-based MTS assay Cytotoxicity against MDCK cells assessed as cell viability by colorimetric formazan-based MTS assay	[PMID: 22459876]
MDCK	CC₅₀	> 220.36 μM Compound: 1-Adamantylamine	Cytotoxicity against MDCK cells by MTT assay Cytotoxicity against MDCK cells by MTT assay	[PMID: 23538114]
MDCK	CC₅₀	> 220.36 μM Compound: 1-Adamantylamine	Cytotoxicity against MDCK cells by MTT assay Cytotoxicity against MDCK cells by MTT assay	[PMID: 22226654]
MDCK	CC₅₀	> 400 μM Compound: Amantadine	Cytotoxicity against dog MDCK cells assessed as reduction in cell viability measured after 5 to 6 days by MTS assay Cytotoxicity against dog MDCK cells assessed as reduction in cell viability measured after 5 to 6 days by MTS assay	[PMID: 28477572]
MDCK	CC₅₀	> 440.99 μM Compound: AMD	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 30292897]
MDCK	IC₅₀	> 50 μM Compound: AMD	Antiviral activity against amantidine-resistant Influenza A virus A/WSN/33(H1N1) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effects after 3 days by CCK-8 assay Antiviral activity against amantidine-resistant Influenza A virus A/WSN/33(H1N1) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effects after 3 days by CCK-8 assay	[PMID: 22332894]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 3 days by MTS assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 3 days by MTS assay	[PMID: 30170320]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by MTS assay Antiviral activity against Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by MTS assay	[PMID: 23117173]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability measured after 3 to 6 days by MTS assay Cytotoxicity against MDCK cells assessed as reduction in cell viability measured after 3 to 6 days by MTS assay	[PMID: 29886325]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as decrease in cell viability after 72 hrs by MTS assay Cytotoxicity against MDCK cells assessed as decrease in cell viability after 72 hrs by MTS assay	[PMID: 29220568]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 28418242]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against MDCK cells after 72 hrs by MTS assay Cytotoxicity against MDCK cells after 72 hrs by MTS assay	[PMID: 25752526]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 25752526]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza A virus A/Virginia/ATCC3/2009 infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus A/Virginia/ATCC3/2009 infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay	[PMID: 24974341]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 72 hrs by MTS assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 72 hrs by MTS assay	[PMID: 24974341]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus (B/HK/05/1972) infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay Antiviral activity against Influenza B virus (B/HK/05/1972) infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay	[PMID: 24974341]
MDCK	CC₅₀	> 500 μM Compound: Amt	Cytotoxicity against MDCK cells assessed as cell viability after 72 hrs by MTS assay Cytotoxicity against MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 24941437]
MDCK	CC₅₀	> 500 μM Compound: Amt	Cytotoxicity against MDCK cells after 72 hrs by MTS assay Cytotoxicity against MDCK cells after 72 hrs by MTS assay	[PMID: 24237039]
MDCK	EC₅₀	> 500 μM Compound: Amt	Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by MTS assay Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by MTS assay	[PMID: 24237039]
MDCK	CC₅₀	> 500 μM Compound: Amt	Cytotoxicity against MDCK cells after 72 hrs by MTS assay Cytotoxicity against MDCK cells after 72 hrs by MTS assay	[PMID: 21466220]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B/HK/5/72 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect measured on day 3 post infection by MTS assay Antiviral activity against Influenza B/HK/5/72 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect measured on day 3 post infection by MTS assay	[PMID: 23149298]
MDCK	CC₅₀	> 500 μM Compound: Amantadine	Cytotoxicity against dog MDCK cells assessed as cell viability by MTS assay Cytotoxicity against dog MDCK cells assessed as cell viability by MTS assay	[PMID: 23117173]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay Antiviral activity against Influenza B virus infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay	[PMID: 32214762]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis Antiviral activity against Influenza B virus (B/HK/5/72) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis	[PMID: 23117173]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 25752526]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza A virus A/Virginia/ATCC3/2009 infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy Antiviral activity against Influenza A virus A/Virginia/ATCC3/2009 infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy	[PMID: 24974341]
MDCK	EC₅₀	> 500 μM Compound: Amantadine	Antiviral activity against Influenza B virus (B/HK/05/1972) infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy Antiviral activity against Influenza B virus (B/HK/05/1972) infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy	[PMID: 24974341]
MDCK	EC₅₀	> 500 μM Compound: Amt	Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by microsocopic analysis Antiviral activity against Influenza B virus (B/Hong Kong/05/1972) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by microsocopic analysis	[PMID: 24237039]
MDCK	EC₅₀	> 80 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytocidal activity after 48 hrs by CellTiter-Glo assay	[PMID: 25147619]
MDCK	CC₅₀	> 80 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo assay	[PMID: 25147619]
MDCK	EC₅₀	0.04 μM Compound: Amantadine	Antiviral activity against Influenza A virus A/Puerto Rico/8/34 expressing wild type M2 proton channel infected in MDCK cells assessed as inhibition of viral replication measured after 10 hrs by miniplaque assay Antiviral activity against Influenza A virus A/Puerto Rico/8/34 expressing wild type M2 proton channel infected in MDCK cells assessed as inhibition of viral replication measured after 10 hrs by miniplaque assay	[PMID: 27187859]
MDCK	EC₅₀	0.054 μg/mL Compound: Amantadine	Antiviral activity against influenza A virus NJH1N1 in MDCK cells by hemadsorption-inhibition assay Antiviral activity against influenza A virus NJH1N1 in MDCK cells by hemadsorption-inhibition assay	[PMID: 9214738]
MDCK	EC₅₀	0.14 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) infected in MDCK cells assessed as virus plaque reduction preincubated for 1 hr followed by compound wash out and incubated for 72 hrs by crystal violet staining Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) infected in MDCK cells assessed as virus plaque reduction preincubated for 1 hr followed by compound wash out and incubated for 72 hrs by crystal violet staining	[PMID: 25899336]
MDCK	EC₅₀	0.2 μg/mL Compound: Amantadine	Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay	[PMID: 20971531]
MDCK	EC₅₀	0.3 μg/mL Compound: Amantadine	Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE Antiviral activity against Influenza A H3N2 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE	[PMID: 20971531]
MDCK	EC₅₀	0.3 μM Compound: amantadine	Antiviral activity against influenza A virus H3N3 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay Antiviral activity against influenza A virus H3N3 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay	[PMID: 23811093]
MDCK	EC₅₀	0.3 μM Compound: 1; AMT	Antiviral activity against Influenza A virus A/udorn/72(H3N2) expressing wild type M2 proton channel infected in MDCK cells assessed as reduction in plaque formation preincubated for 30 mins followed by infection measured on day 2 post infection by naphth Antiviral activity against Influenza A virus A/udorn/72(H3N2) expressing wild type M2 proton channel infected in MDCK cells assessed as reduction in plaque formation preincubated for 30 mins followed by infection measured on day 2 post infection by naphth	[PMID: 26771709]
MDCK	EC₅₀	0.34 μM Compound: 1	Antiviral activity against Influenza A virus A2/Taiwan/1/64(H2N2) infected in MDCK cells expressing wild type M2 after overnight incubation by fluorescence-based miniplaque assay Antiviral activity against Influenza A virus A2/Taiwan/1/64(H2N2) infected in MDCK cells expressing wild type M2 after overnight incubation by fluorescence-based miniplaque assay	[PMID: 24793875]
MDCK	EC₅₀	0.45 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/HK/7/1987(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy Antiviral activity against Influenza A virus (A/HK/7/1987(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy	[PMID: 24974341]
MDCK	IC₅₀	0.5 μM Compound: Amantadine	Antiviral activity against wild type M2 ion channel expressing Influenza A virus (A/Hong Kong/1968(H3N2)) infected in MDCK cells preincubated for 30 mins followed by viral infection measured after 72 hrs by CCK-8 assay Antiviral activity against wild type M2 ion channel expressing Influenza A virus (A/Hong Kong/1968(H3N2)) infected in MDCK cells preincubated for 30 mins followed by viral infection measured after 72 hrs by CCK-8 assay	10.1039/C4MD00515E
MDCK	EC₅₀	0.5 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/HK/7/1987(H3N2)) infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/HK/7/1987(H3N2)) infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay	[PMID: 24974341]
MDCK	EC₅₀	0.6 μM Compound: AMT	Antiviral activity against Influenza A virus (A/Taiwan/1/1986(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis Antiviral activity against Influenza A virus (A/Taiwan/1/1986(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis	[PMID: 23419738]
MDCK	EC₅₀	0.82 μM Compound: Amt	Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by MTS assay	[PMID: 24237039]
MDCK	EC₅₀	0.84 μM Compound: Amt	Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by microsocopic analysis Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by microsocopic analysis	[PMID: 24237039]
MDCK	IC₅₀	0.92 μg/mL Compound: Amantadine	Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 3 days by XTT assay Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 3 days by XTT assay	[PMID: 19691312]
MDCK	EC₅₀	1.2 μM Compound: Amantadine	Antiviral activity against IInfluenza A virus H3N2 A/HK/7/87 infected in MDCK cells after 4 days by MTS assay Antiviral activity against IInfluenza A virus H3N2 A/HK/7/87 infected in MDCK cells after 4 days by MTS assay	[PMID: 31753804]
MDCK	EC₅₀	1.4 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 25899336]
MDCK	EC₅₀	1.4 μM Compound: Amt	Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 24941437]
MDCK	EC₅₀	1.4 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 25899336]
MDCK	EC₅₀	1.4 μM Compound: Amt	Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza A virus (A/HK/7/87(H3N2)) harboring wild type M2 channel infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 24941437]
MDCK	EC₅₀	1.5 μM Compound: Amantadine	Antiviral activity against Influenza A virus H3N2 infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay Antiviral activity against Influenza A virus H3N2 infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay	[PMID: 32214762]
MDCK	EC₅₀	1.8 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 25752526]
MDCK	EC₅₀	1.9 μM Compound: AMT	Antiviral activity against Influenza A virus (A/Hong kong/8/1968(H2N3)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis Antiviral activity against Influenza A virus (A/Hong kong/8/1968(H2N3)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity at 1 hr post-infection measured after 3 days by fluorescein-diacetate based fluorimetric analysis	[PMID: 23419738]
MDCK	EC₅₀	1.98 μM Compound: amantadine	Antiviral activity against influenza A/Hong Kong/7/87 (H3N2) in MDCK cells assessed as inhibition of virus-induced cytopathic effect by MTS assay Antiviral activity against influenza A/Hong Kong/7/87 (H3N2) in MDCK cells assessed as inhibition of virus-induced cytopathic effect by MTS assay	[PMID: 17588747]
MDCK	IC₅₀	10.78 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells assessed as inhibition of viral replication after 2 to 3 days by crystal violet staining-based plaque reduction assay Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells assessed as inhibition of viral replication after 2 to 3 days by crystal violet staining-based plaque reduction assay	[PMID: 24803363]
MDCK	EC₅₀	100 μM Compound: AMN	Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by visual cytopathic effect scoring method	[PMID: 19281225]
MDCK	EC₅₀	101 μg/mL Compound: Amantadin	Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days	[PMID: 20724039]
MDCK	EC₅₀	11 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/Ishikawa/7/82 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against Influenza A virus (A/Ishikawa/7/82 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	EC₅₀	111 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as reduction in host cell viability after 72 hrs by MTS assay	[PMID: 24974341]
MDCK	EC₅₀	115 μg/mL Compound: Amantadin	Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as cell viability after 2 days by MTS assay Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as cell viability after 2 days by MTS assay	[PMID: 20724039]
MDCK	EC₅₀	117 μg/mL Compound: Amantadin	Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as protection against virus-induced cytopathogenicity after 2 days	[PMID: 20724039]
MDCK	IC₅₀	13 μM Compound: Amantadine	Antiviral activity against Influenza A virus A/WSN/1933 (H1N1) infected in MDCK cells assessed as protection against virus induced cytopathic effect after 40 hrs by Celltiter-Glo assay Antiviral activity against Influenza A virus A/WSN/1933 (H1N1) infected in MDCK cells assessed as protection against virus induced cytopathic effect after 40 hrs by Celltiter-Glo assay	[PMID: 26686929]
MDCK	EC₅₀	143.84 μM Compound: Amantadine	Antiviral activity against influenza B virus B/SZ/155 infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay Antiviral activity against influenza B virus B/SZ/155 infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay	[PMID: 26686929]
MDCK	IC₅₀	15 μg/mL Compound: amantadine	Antiviral activity against Influenza A virus (A/WSN/33/London (H1N1)) in MDCK cells after 72 hrs by neutral red dye uptake assy Antiviral activity against Influenza A virus (A/WSN/33/London (H1N1)) in MDCK cells after 72 hrs by neutral red dye uptake assy	[PMID: 17602586]
MDCK	EC₅₀	154 μM Compound: Amantadine	Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay	[PMID: 22459876]
MDCK	EC₅₀	17 μM Compound: amantadine	Antiviral activity against influenza A virus H1N1 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay Antiviral activity against influenza A virus H1N1 subtype infected in dog MDCK cells assessed as reduction of virus-induced cytopathogenicity by MTS assay	[PMID: 23811093]
MDCK	EC₅₀	18 μg/mL Compound: Amantadine	Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity by visual scoring of CPE	[PMID: 20971531]
MDCK	EC₅₀	2 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity by MTS assay Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity by MTS assay	[PMID: 20805012]
MDCK	EC₅₀	2 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza A virus (A/Hong Kong/7/1987(H3N2)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 25752526]
MDCK	EC₅₀	2 μM Compound: amantadine	Antiviral activity against amantadine-sensitive Influenza A virus (A/Ned/378/05 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against amantadine-sensitive Influenza A virus (A/Ned/378/05 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	IC₅₀	2.22 μM Compound: AMD	Antiviral activity against Influenza A virus A/HK/8/68(H3N2) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effects after 3 days by CCK-8 assay Antiviral activity against Influenza A virus A/HK/8/68(H3N2) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effects after 3 days by CCK-8 assay	[PMID: 22332894]
MDCK	EC₅₀	2.3 μM Compound: Amantadine	Antiviral activity against Influenza A/HK/7/87 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect measured on day 3 post infection by MTS assay Antiviral activity against Influenza A/HK/7/87 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect measured on day 3 post infection by MTS assay	[PMID: 23149298]
MDCK	IC₅₀	2.38 μM Compound: 1-Adamantylamine	Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells assessed as inhibition of viral replication by plaque reduction assay Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells assessed as inhibition of viral replication by plaque reduction assay	[PMID: 22226654]
MDCK	EC₅₀	2.7 μM Compound: amantadine	Antiviral activity against influenza virus A/Hong Kong/7/87 H3N2 in MDCK cells assessed as effect on virus-induced cytopathogenicity by MTS assay Antiviral activity against influenza virus A/Hong Kong/7/87 H3N2 in MDCK cells assessed as effect on virus-induced cytopathogenicity by MTS assay	[PMID: 18954995]
MDCK	EC₅₀	2.77 μM Compound: 1	Antiviral activity against Influenza A virus A/Victoria/3/75(H3N2) infected in MDCK cells expressing wild type M2 after overnight incubation by fluorescence-based miniplaque assay Antiviral activity against Influenza A virus A/Victoria/3/75(H3N2) infected in MDCK cells expressing wild type M2 after overnight incubation by fluorescence-based miniplaque assay	[PMID: 24793875]
MDCK	EC₅₀	208 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by MTS assay Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by MTS assay	[PMID: 23117173]
MDCK	IC₅₀	21.11 μM Compound: 1	Antiviral activity against amantadine-sensitive Influenza A virus A/Hong Kong/8/68 (H3N2) infected in MDCK cells assessed as host cell survival after 72 hrs by CCK8 method Antiviral activity against amantadine-sensitive Influenza A virus A/Hong Kong/8/68 (H3N2) infected in MDCK cells assessed as host cell survival after 72 hrs by CCK8 method	[PMID: 21094565]
MDCK	EC₅₀	212 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect after 72 hrs by microscopy	[PMID: 24974341]
MDCK	CC₅₀	218.92 μM Compound: Amantadine	Cytotoxicity against MDCK cells after 72 hrs by MTT assay Cytotoxicity against MDCK cells after 72 hrs by MTT assay	[PMID: 24803363]
MDCK	EC₅₀	22.5 μM Compound: 1	Antiviral activity against influenza A virus A/WSN/33-S31N infected in dog MDCK cells after 2 days by naphthalene black dye staining based plaque reduction assay Antiviral activity against influenza A virus A/WSN/33-S31N infected in dog MDCK cells after 2 days by naphthalene black dye staining based plaque reduction assay	[PMID: 23437766]
MDCK	EC₅₀	22.5 μM Compound: 1; AMT	Antiviral activity against Influenza A virus A/WSN/33(H1N1) expressing M2 proton channel S31N mutant infected in MDCK cells assessed as reduction in plaque formation preincubated for 30 mins followed by infection measured on day 2 post infection by naphth Antiviral activity against Influenza A virus A/WSN/33(H1N1) expressing M2 proton channel S31N mutant infected in MDCK cells assessed as reduction in plaque formation preincubated for 30 mins followed by infection measured on day 2 post infection by naphth	[PMID: 26771709]
MDCK	EC₅₀	224 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis	[PMID: 23117173]
MDCK	EC₅₀	224 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 25752526]
MDCK	EC₅₀	24 μM Compound: 1	Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells expressing M2 V27T/S31N mutant after overnight incubation by fluorescence-based miniplaque assay Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells expressing M2 V27T/S31N mutant after overnight incubation by fluorescence-based miniplaque assay	[PMID: 24793875]
MDCK	EC₅₀	24 μM Compound: 1	Antiviral activity against Influenza A virus A/WS/33(H1N1) infected in MDCK cells expressing M2 S31N mutant after overnight incubation by fluorescence-based miniplaque assay Antiviral activity against Influenza A virus A/WS/33(H1N1) infected in MDCK cells expressing M2 S31N mutant after overnight incubation by fluorescence-based miniplaque assay	[PMID: 24793875]
MDCK	EC₅₀	240 μM Compound: 1	Antiviral activity against Influenza A virus (A/California/07/2009(H1N1)) infected in MDCK cells expressing M2 S31N mutant after overnight incubation by fluorescence-based miniplaque assay Antiviral activity against Influenza A virus (A/California/07/2009(H1N1)) infected in MDCK cells expressing M2 S31N mutant after overnight incubation by fluorescence-based miniplaque assay	[PMID: 24793875]
MDCK	CC₅₀	284 μM Compound: Amantadine	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against MDCK cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30746065]
MDCK	CC₅₀	284.1 μM Compound: Amantadine	Cytotoxicity against MDCK cells after 48 hrs by MTT assay Cytotoxicity against MDCK cells after 48 hrs by MTT assay	[PMID: 30108810]
MDCK	EC₅₀	3.4 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/HK/7/87 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against Influenza A virus (A/HK/7/87 (H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	EC₅₀	3.8 μM Compound: Amantadine	Antiviral activity against Influenza A virus H3N2 A/HK/7/87 infected in MDCK cells assessed as reduction in virus induced cytopathic effect after 4 days by microscopy based method Antiviral activity against Influenza A virus H3N2 A/HK/7/87 infected in MDCK cells assessed as reduction in virus induced cytopathic effect after 4 days by microscopy based method	[PMID: 31753804]
MDCK	IC₅₀	3.84 μM Compound: 1-Adamantylamine	Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells by plaque reduction assay Antiviral activity against Influenza A virus (A/Vietnam/1194/2004(H5N1)) infected in MDCK cells by plaque reduction assay	[PMID: 23538114]
MDCK	EC₅₀	30 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as reduction of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 25899336]
MDCK	EC₅₀	30 μM Compound: Amt	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) harboring M2 S31N/V27T double mutant channel infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 72 hrs by microscopic analysis Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) harboring M2 S31N/V27T double mutant channel infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 72 hrs by microscopic analysis	[PMID: 24941437]
MDCK	IC₅₀	337.2 μM Compound: amantadine	Antiviral activity against amantadine-resistant Influenza A virus A/WSN/33(H1N1) expressing M2 S31N mutant ion channel infected in MDCK cells assessed as protection against virus induced cytopathic effect after 48 hrs by microscopic analysis Antiviral activity against amantadine-resistant Influenza A virus A/WSN/33(H1N1) expressing M2 S31N mutant ion channel infected in MDCK cells assessed as protection against virus induced cytopathic effect after 48 hrs by microscopic analysis	[PMID: 26722757]
MDCK	EC₅₀	34 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 25899336]
MDCK	EC₅₀	34 μM Compound: Amt	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) harboring M2 S31N/V27T double mutant channel infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) harboring M2 S31N/V27T double mutant channel infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 24941437]
MDCK	EC₅₀	34 μM Compound: Amt	Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) harboring M2 channel S31N and V27T mutant infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by microsocopic analysis Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) harboring M2 channel S31N and V27T mutant infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by microsocopic analysis	[PMID: 24237039]
MDCK	EC₅₀	38 μM Compound: Amt	Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) harboring M2 channel S31N and V27T mutant infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) harboring M2 channel S31N and V27T mutant infected in MDCK cells assessed as reduction of cytopathic effect after 72 hrs by MTS assay	[PMID: 24237039]
MDCK	EC₅₀	4.1 μM Compound: Amantadine	Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus-induced cytopathogenicity after 4 days by colorimetric formazan-based MTS assay	[PMID: 22459876]
MDCK	EC₅₀	4.28 μM Compound: Amantadine	Antiviral activity against amantadine-resistant influenza A virus JX/312 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay Antiviral activity against amantadine-resistant influenza A virus JX/312 (H3N2) infected in dog MDCK cells assessed as inhibition of virus induced cytopathic effect after 40 hrs by Cell-Titer-Glo assay	[PMID: 26686929]
MDCK	EC₅₀	44 μg/mL Compound: Amantadine	Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay Antiviral activity against Influenza A H1N1 virus subtype infected in MDCK cells assessed as inhibition of virus induced cytopathicity after 2 to 4 days by MTS assay	[PMID: 20971531]
MDCK	EC₅₀	44 μM Compound: Amantadine	Antiviral activity against Influenza A virus A/PR/8/34 infected in MDCK cells assessed as reduction of virus induced cytopathic effect measured by visual scoring of cytopathic effect Antiviral activity against Influenza A virus A/PR/8/34 infected in MDCK cells assessed as reduction of virus induced cytopathic effect measured by visual scoring of cytopathic effect	[PMID: 27112451]
MDCK	EC₅₀	453 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay Antiviral activity against Influenza A virus (A/Puerto Rico/8/34(H1N1)) infected in MDCK cells assessed as cell viability after 72 hrs by MTS assay	[PMID: 25752526]
MDCK	EC₅₀	47 μM Compound: Amantadine	Antiviral activity against Influenza A virus A/PR/8/34 infected in MDCK cells assessed as reduction of virus-induced cytopathic effect measured after 4 days by formazan-based MTS assay Antiviral activity against Influenza A virus A/PR/8/34 infected in MDCK cells assessed as reduction of virus-induced cytopathic effect measured after 4 days by formazan-based MTS assay	[PMID: 27112451]
MDCK	EC₅₀	5 μM Compound: Amantadine	Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days	[PMID: 22459876]
MDCK	EC₅₀	5.1 μM Compound: amantadine	Antiviral activity against amantadine-sensitive Influenza A virus (A/FM/1/47 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against amantadine-sensitive Influenza A virus (A/FM/1/47 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	EC₅₀	53 μM Compound: amantadine	Antiviral activity against amantadine-resistant Influenza A virus (A/PR/8/34 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against amantadine-resistant Influenza A virus (A/PR/8/34 (H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	IC₅₀	56 μg/mL Compound: Amantadine	Cytotoxicity against MDCK cells by neutral red assay Cytotoxicity against MDCK cells by neutral red assay	[PMID: 9214738]
MDCK	EC₅₀	58 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by microscopic analysis	[PMID: 23117173]
MDCK	EC₅₀	61 μM Compound: amantadine	Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis Antiviral activity against Influenza A virus (A/X-31(H3N2)) infected in MDCK cells assessed as virus-induced cytopathic effect measured 3 days post infection by microscopic analysis	[PMID: 24900429]
MDCK	ED₅₀	64.2 μM Compound: Amantadine	Antiviral activity against rimantidine, amantadine-resistant Influenza A virus (A/California/07/09(H1N1)) pdm09 infected in MDCK cells assessed as inhibition of viral replication by hemagglutinin titer assay Antiviral activity against rimantidine, amantadine-resistant Influenza A virus (A/California/07/09(H1N1)) pdm09 infected in MDCK cells assessed as inhibition of viral replication by hemagglutinin titer assay	[PMID: 24631360]
MDCK	EC₅₀	64.2 μM Compound: Amantadine, Symmetrel	Antiviral activity against Influenza virus A/California/07/09 (H1N1)pdm09 infected in MDCK cells assessed as inhibition of viral replication incubated for 1 hr prior to viral infection measured after 48 hrs by hemagglutination reaction Antiviral activity against Influenza virus A/California/07/09 (H1N1)pdm09 infected in MDCK cells assessed as inhibition of viral replication incubated for 1 hr prior to viral infection measured after 48 hrs by hemagglutination reaction	[PMID: 23993669]
MDCK	EC₅₀	65.5 μM Compound: Amantadine	Antiviral activity against Unidentified Influenza A virus (H1N1) infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay Antiviral activity against Unidentified Influenza A virus (H1N1) infected MDCK cells assessed as reduction of virus-induced cytopathic effect after 3 days by colorimetric formazan-based MTS assay	[PMID: 32214762]
MDCK	EC₅₀	7.6 μM Compound: Amantadine	Antiviral activity against Influenza A/PR/8/34 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect measured on day 3 post infection by MTS assay Antiviral activity against Influenza A/PR/8/34 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect measured on day 3 post infection by MTS assay	[PMID: 23149298]
MDCK	EC₅₀	75 μM Compound: Amantadine	Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by MTS assay Antiviral activity against Influenza A virus (A/PR/8/34(H1N1)) infected in MDCK cells assessed as virus-induced cytopathic effect after 3 days by MTS assay	[PMID: 23117173]
MDCK	EC₅₀	75.3 μM Compound: AMN	Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method Antiviral activity against influenza H3N2 virus in MDCK cells assessed as reduction of virus-induced cytopathicity by MTS method	[PMID: 19281225]
MDCK	EC₅₀	77 μM Compound: amantadine	Antiviral activity against influenza virus A/Puerto Rico/8/34 H1N1 in MDCK cells assessed as effect on virus-induced cytopathogenicity by MTS assay Antiviral activity against influenza virus A/Puerto Rico/8/34 H1N1 in MDCK cells assessed as effect on virus-induced cytopathogenicity by MTS assay	[PMID: 18954995]
MDCK	EC₅₀	87 μg/mL Compound: Amantadin	Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as cell viability after 2 days by MTS assay Antiviral activity against influenza A virus H1N1 infected in MDCK cells assessed as cell viability after 2 days by MTS assay	[PMID: 20724039]
MDCK	EC₅₀	89 μM Compound: Amantadine	Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days Antiviral activity against Influenza A virus H1N1 infected in MDCK cells assessed as reduction visual scoring of virus-induced cytopathogenicity after 4 days	[PMID: 22459876]
Oocyte	IC₅₀	16 μM Compound: Amt	Inhibition of Influenza A virus (A/Udorn/72) wild type matrix protein 2 expressed in xenopus oocytes after 2 mins by two-electrode voltage clamp assay Inhibition of Influenza A virus (A/Udorn/72) wild type matrix protein 2 expressed in xenopus oocytes after 2 mins by two-electrode voltage clamp assay	[PMID: 21466220]
Oocyte	IC₅₀	199.9 μM Compound: Amt	Inhibition of Influenza A virus (A/Udorn/72) matrix protein 2 S31N mutant expressed in xenopus oocytes after 2 mins by two-electrode voltage clamp assay Inhibition of Influenza A virus (A/Udorn/72) matrix protein 2 S31N mutant expressed in xenopus oocytes after 2 mins by two-electrode voltage clamp assay	[PMID: 21466220]
Vero	CC₅₀	2019.7 μM Compound: Amantadine	Cytotoxicity against African green monkey Vero cells measured after 48 hrs by crystal violet staining based colorimetric method Cytotoxicity against African green monkey Vero cells measured after 48 hrs by crystal violet staining based colorimetric method	[PMID: 31054861]

In Vitro

Amantadine (0-500 µM, 26 h) inhibits SARS-CoV-2 replication, with IC₅₀ concentrations between 83 and 119 µM^[4].
Amantadine (0-100 µg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC‑7721 cells^[6].
Amantadine (0-75 µg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis^[6].
Amantadine (0-75 µg/mL, 48 h) reduces the levels of the cell cycle‑related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	Vero E6 cells
Concentration:	500 µM, 100 µM, 20 µM, 4 µM, and 8 nM
Incubation Time:	26 h
Result:	Caused a concentration-dependent reduction (IC₅₀=83 µM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 µM. Caused a concentration-dependent reduction (IC₅₀=119 µM) of viral nucleic acids in the cytosol 26 h after infection.

Cell Proliferation Assay^[6]

Cell Line:	Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells)
Concentration:	0, 1, 2, 5, 10, 25, 50 and 100 µg/mL
Incubation Time:	24, 48 and 72 h
Result:	Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.

Cell Cycle Analysis^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.

Apoptosis Analysis^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.

Western Blot Analysis^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells.

RT-PCR^[6]

Cell Line:	HepG2 and SMMC-7721 cells
Concentration:	0, 10, 25, 50 and 75 µg/mL
Incubation Time:	48 h
Result:	Revealed an increase in Bax and decrease in Bcl-2 genes.

In Vivo

Amantadine (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group)^[5]
Dosage:	25 mg/kg
Administration:	IP, once daily for 3 days (the first dose at 15 min before surgery)
Result:	Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.

Clinical Trial

Molecular Weight

151.25

Formula

C₁₀H₁₇N

CAS No.

768-94-5

Appearance

Solid

Color

White to off-white

SMILES

NC12C[C@H]3C[C@@H](C2)C[C@@H](C1)C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 6.48 mg/mL (42.84 mM; ultrasonic and warming and adjust pH to 4 with 1 M HCL and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6116 mL	33.0578 mL	66.1157 mL
5 mM	1.3223 mL	6.6116 mL	13.2231 mL
10 mM	0.6612 mL	3.3058 mL	6.6116 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (6.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (3.31 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (248 KB) HNMR (228 KB) RP-HPLC (214 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Suzuki H, et al. Emergence of amantadine-resistant influenza A viruses: epidemiological study. J Infect Chemother. 2003;9(3):195-200. [Content Brief]

[2]. Hubsher G, et al. Amantadine: the journey from fighting flu to treating Parkinson disease. Neurology. 2012;78(14):1096-1099. [Content Brief]

[3]. Donald F Smee, et al. A review of compounds exhibiting anti-orthopoxvirus activity in animal models. Antiviral Res. 2003 Jan;57(1-2):41-52. [Content Brief]

[4]. Fink K, et al. Amantadine Inhibits SARS-CoV-2 In Vitro. Viruses. 2021 Mar 24;13(4):539. [Content Brief]

[5]. Zhang J, et al. Amantadine alleviates postoperative cognitive dysfunction possibly by increasing glial cell line-derived neurotrophic factor in rats. Anesthesiology. 2014 Oct;121(4):773-85. [Content Brief]

[6]. Lan Z, et al. Amantadine inhibits cellular proliferation and induces the apoptosis of hepatocellular cancer cells in vitro. Int J Mol Med. 2015;36(3):904-910. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.6116 mL	33.0579 mL	66.1157 mL	165.2893 mL
	5 mM	1.3223 mL	6.6116 mL	13.2231 mL	33.0579 mL
	10 mM	0.6612 mL	3.3058 mL	6.6116 mL	16.5289 mL
	15 mM	0.4408 mL	2.2039 mL	4.4077 mL	11.0193 mL
	20 mM	0.3306 mL	1.6529 mL	3.3058 mL	8.2645 mL
	25 mM	0.2645 mL	1.3223 mL	2.6446 mL	6.6116 mL
	30 mM	0.2204 mL	1.1019 mL	2.2039 mL	5.5096 mL
	40 mM	0.1653 mL	0.8264 mL	1.6529 mL	4.1322 mL

Amantadine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Amantadine (Synonyms: 1-Adamantanamine; 1-Aminoadamantane)

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Amantadine (Synonyms: 1-Adamantanamine; 1-Aminoadamantane)

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Amantadine Related Antibodies

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•Related Screening Libraries:

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View All CDK Isoform Specific Products:

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