2. GPCR/G Protein MAPK/ERK Pathway Stem Cell/Wnt
  3. GPR35 ERK
  4. Pamoic acid disodium

Pamoic acid disodium is a potent GPR35 agonist with an EC50 value of 79 nM. Pamoic acid disodium induces GPR35 internalization and activates ERK1/2 with EC50 values of 22 nM and 65 nM, respectively. Pamoic acid disodium potently recruits β-arrestin2 to GPR35 and has an antinociceptive effect.

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Pamoic acid disodium Chemical Structure

Pamoic acid disodium Chemical Structure

CAS No. : 6640-22-8

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10 mM * 1 mL in DMSO
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Based on 2 publication(s) in Google Scholar

Other Forms of Pamoic acid disodium:

Pamoic acid disodium Related Antibodies

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Description

Pamoic acid disodium is a potent GPR35 agonist with an EC50 value of 79 nM. Pamoic acid disodium induces GPR35 internalization and activates ERK1/2 with EC50 values of 22 nM and 65 nM, respectively. Pamoic acid disodium potently recruits β-arrestin2 to GPR35 and has an antinociceptive effect[1].

IC50 & Target

EC50: 79 nM (GPR35), 65 nM (ERK1/2)[1]
In Vitro

Pamoic acid (0.1 nM-0.1 mM) induces GPR35a internalization with an EC50 value of 22 nM[1].
Pamoic acid (0-10 μM) induces ERK1/2 activation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pamoic acid disodium Related Antibodies

Western Blot Analysis[1]

Cell Line: U2OS cells
Concentration: 1, 10, 100 nM and 1, 10 μM
Incubation Time: 15 min
Result: A concentration-dependent activation of ERK1/2 was observed with an EC50 of 65 nM.
In Vivo

Pamoic acid disodium (0-100 mg/kg; s.c.; once) induces a dose-related antinociception in the abdominal constriction test[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss-Webster mice (30–35 g)[1]
Dosage: 25, 50, and 100 mg/kg
Administration: Subcutaneous injection, once
Result: Elicited dose-related antinociception, the dose causing 50% antinociception being 40.5 mg/kg.
Molecular Weight

432.33

Formula

C23H14Na2O6
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

OC1=C(C(C=CC=C2)=C2C=C1C(O[Na])=O)CC(C(C=CC=C3)=C3C=C4C(O[Na])=O)=C4O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (231.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3130 mL 11.5652 mL 23.1305 mL
5 mM 0.4626 mL 2.3130 mL 4.6261 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 7.5 mg/mL (17.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 7.5 mg/mL (17.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

COA (260 KB) HNMR (263 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3130 mL 11.5652 mL 23.1305 mL 57.8262 mL
5 mM 0.4626 mL 2.3130 mL 4.6261 mL 11.5652 mL
10 mM 0.2313 mL 1.1565 mL 2.3130 mL 5.7826 mL
15 mM 0.1542 mL 0.7710 mL 1.5420 mL 3.8551 mL
20 mM 0.1157 mL 0.5783 mL 1.1565 mL 2.8913 mL
25 mM 0.0925 mL 0.4626 mL 0.9252 mL 2.3130 mL
30 mM 0.0771 mL 0.3855 mL 0.7710 mL 1.9275 mL
40 mM 0.0578 mL 0.2891 mL 0.5783 mL 1.4457 mL
50 mM 0.0463 mL 0.2313 mL 0.4626 mL 1.1565 mL
60 mM 0.0386 mL 0.1928 mL 0.3855 mL 0.9638 mL
80 mM 0.0289 mL 0.1446 mL 0.2891 mL 0.7228 mL
100 mM 0.0231 mL 0.1157 mL 0.2313 mL 0.5783 mL
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Pamoic acid disodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pamoic acid disodium6640-22-8GPR35ERKG Protein-Coupled Receptor 35Extracellular signal regulated kinasesinactiveβ-arrestin2ERK1ERK2antinociceptiveInhibitorinhibitorinhibit

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