2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor Anti-infection
  3. LXR Bacterial
  4. Saikosaponin A

Saikosaponin A is the main active ingredient in Bupleurum chinense, which can regulate lipid metabolism and promote cholesterol efflux in early atherosclerosis. In addition, Saikosaponin A may also serve as a potential peroxisome proliferator-activated receptor γ (PPAR-γ) agonist, significantly promoting the expression of PPAR-γ. Saikosaponin A can be used in the study of hyperlipidemic pancreatitis.

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Saikosaponin A Chemical Structure

Saikosaponin A Chemical Structure

CAS No. : 20736-09-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 52 In-stock
Solution
10 mM * 1 mL in DMSO USD 52 In-stock
Solid
5 mg USD 35 In-stock
10 mg USD 60 In-stock
25 mg USD 125 In-stock
50 mg USD 210 In-stock
100 mg USD 360 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Saikosaponin A:

Saikosaponin A Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Saikosaponin A

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Saikosaponin A is the main active ingredient in Bupleurum chinense, which can regulate lipid metabolism and promote cholesterol efflux in early atherosclerosis. In addition, Saikosaponin A may also serve as a potential peroxisome proliferator-activated receptor γ (PPAR-γ) agonist, significantly promoting the expression of PPAR-γ. Saikosaponin A can be used in the study of hyperlipidemic pancreatitis[1][2][3].

In Vitro

Saikosaponin A (5-15 μM, 24 h) did not affect the viability of human osteoarthritis chondrocytes[1].
Saikosaponin A (5-15 μM, 24 h) inhibits IL-1β (HY-P73149)-induced NO and PGE2 production and NF-κB activation[1].
Saikosaponin A (5-15 μM, 24 h) up-regulates the expression of LXRα in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Saikosaponin A Related Antibodies

Western Blot Analysis

Cell Line: Human osteoarthritis chondrocytes
Concentration: 5, 10, 15 μM
Incubation Time: 24 h
Result: Inhibited the levels of phosphorylation of NF-κB p65 and IκBα induced by IL-1β.
Up-regulated the expression of LXRα in a dose-dependent manner.
In Vivo

Saikosaponin A (1-10 mg/kg, i.p, 7 d) attenuates the morphological changes in the small intestine induced by 5-FU[2].
Saikosaponin-A (1-10 mg/kg, i.p, 7 d) enhances the decrease of antioxidant enzymes in intestinal tissue caused by 5-FU[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-FU (HY-90006)-induced mucositis in BALB/c mice modle[2]
Dosage: 1, 5, 10 mg/kg/day, 7 d
Administration: injected intraperitoneally (i.p)
Result: Reduced villus blunting, crypt cells apoptosis, and inflammatory cell infiltration.
Decreased concentration of GSH, GST, Catalase, and SOD in intestinal tissue.
Molecular Weight

780.98

Formula

C42H68O13
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@H]1O[C@](O[C@@H]2[C@@H](O)[C@@](O[C@@H]3[C@@](C)(CO)[C@@](CC[C@]4(C)[C@]5([H])C=C[C@@]6(OC7)[C@@]4(C)C[C@H](O)[C@]87[C@@]6([H])CC(C)(C)CC8)([H])[C@]5(C)CC3)([H])O[C@H](C)[C@@H]2O)([H])[C@H](O)[C@@H](O)[C@@H]1O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (128.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2804 mL 6.4022 mL 12.8044 mL
5 mM 0.2561 mL 1.2804 mL 2.5609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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Volume (final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 10 mg/mL (12.80 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.31%

COA (253 KB) LCMS (100 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2804 mL 6.4022 mL 12.8044 mL 32.0111 mL
5 mM 0.2561 mL 1.2804 mL 2.5609 mL 6.4022 mL
10 mM 0.1280 mL 0.6402 mL 1.2804 mL 3.2011 mL
15 mM 0.0854 mL 0.4268 mL 0.8536 mL 2.1341 mL
20 mM 0.0640 mL 0.3201 mL 0.6402 mL 1.6006 mL
25 mM 0.0512 mL 0.2561 mL 0.5122 mL 1.2804 mL
30 mM 0.0427 mL 0.2134 mL 0.4268 mL 1.0670 mL
40 mM 0.0320 mL 0.1601 mL 0.3201 mL 0.8003 mL
50 mM 0.0256 mL 0.1280 mL 0.2561 mL 0.6402 mL
60 mM 0.0213 mL 0.1067 mL 0.2134 mL 0.5335 mL
80 mM 0.0160 mL 0.0800 mL 0.1601 mL 0.4001 mL
100 mM 0.0128 mL 0.0640 mL 0.1280 mL 0.3201 mL
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Saikosaponin A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Saikosaponin A20736-09-8LXRBacterialLiver X receptorInhibitorinhibitorinhibit

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