From 11:00 pm to 12:00 pm EST ( 8:00 pm to 9:00 pm PST ) on January 6th, the website will be under maintenance. We are sorry for the inconvenience. Please arrange your schedule properly.
BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm .
5'-GMPS is an analogue of 5'-GMP and a substrate, competitive inhibitor or regulator of enzymes that interact with 5'-GMP. 5'-GMPS is suitable as a primer of RNA synthesis by T7 RNA polymerase .
Setrobuvir (ANA598) is an orally active non-nucleosidic HCV NS5B polymerase inhibitor. ANA-598 inhibits both de novo RNA synthesis and primer extension, with IC50s between 4 and 5 nM. Setrobuvir also shows excellent binding affinity to SARS-CoV-2 RdRp and induces RdRp inhibition .
5-fluoro-dCTP is a fluorinated pyrimidine dNTP that can be used as a substrate for the incorporation of fluorine modification into specific DNA sequences by primer extension (PEX) catalyzed by Pwo polymerase .
5-fluoro-dCTP sodium is a fluorinated pyrimidine dNTP that can be used as a substrate for the incorporation of fluorine modification into specific DNA sequences by primer extension (PEX) catalyzed by Pwo polymerase .
L-697639 is an inhibitor for HIV-1 reverse transcriptase (HIV-1 RT) with IC50 of 20-400 nM (in a template-primer-dependent manner). L-697639 exhibits antiviral activity, that inhibits 95% HIV-1 infection at concentrations of 12-200 nM in human T lymphocyte cultures .
Limochromin is a pigment produced by Streptomyces limosus that strongly inhibits the reverse transcriptase of avian myeloid leukemia virus (AMV). The inhibitory activity of limochromin is not affected by changes in the concentration of template-primer, substrate or carrier protein, but its inhibitory effect is significantly reduced when the concentration of AMV reverse transcriptase increases. Limochromin can be used to develop antiviral treatments .
Fagaronine chloride is an alkaloid with inhibitory activity against reverse transcriptase and topoisomerase I. Fagaronine chloride can effectively inhibit the reverse transcriptase of RSii tumor virus at a concentration of 6-60 μg/mL. Fagaronine chloride rapidly blocks the synthesis of DNA polymerase by interacting with the template primer. Fagaronine chloride has shown anti-tumor potential, especially in the study of retroviral infection .
T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates .
Fludarabine triphosphate (F-ara-ATP), the active metabolite of Fludarabine (HY-B0069), is a potent, noncompetitive and specific inhibitor of DNA primase, with an IC50 of 2.3 μM and a Ki of 6.1 μM. Fludarabine triphosphate inhibits DNA synthesis by blocking DNA primase and primer RNA formation. Fludarabine triphosphate inhibits ribonucleotide reductase and DNA polymerase and ultimately leads to cellular apoptosis .
Fludarabine triphosphate (F-ara-ATP) trisodium, the active metabolite of Fludarabine (HY-B0069), is a potent, noncompetitive and specific inhibitor of DNA primase, with an IC50 of 2.3 μM and a Ki of 6.1 μM. Fludarabine triphosphate trisodium inhibits DNA synthesis by blocking DNA primase and primer RNA formation. Fludarabine triphosphate trisodium inhibits ribonucleotide reductase and DNA polymerase and ultimately leads to cellular apoptosis .
13-TP is an inhibitor of SARS-CoV-2. 13-TP effectively inhibits the SARS-CoV-2 central replication transcription complex (C-RTC, nsp12-nsp7-nsp82) catalyzed in vitro RNA synthesis. 13-TP completely inhibits the RdRp polymerization activity. 13-TP blocks the full extension of some of the primer RNA .
2A3 is a highly efficient and precise SHAPE reagent with powerful biomembrane penetration capabilities, suitable for both in vivo and in vitro RNA structure detection. 2A3 can not only effectively probe most RNA loops but also better capture local nucleotide dynamics, thus enabling it to readily detect regions of ribonucleoprotein complexes that are typically inaccessible to other compounds .
Dehydroaltenusin is a small molecule selective inhibitor of eukaryotic DNA polymerase α, a type of antibiotic produced by a fungus with an IC50 value of 0.68 μM. The inhibitory mode of action of dehydroaltenusin against mammalian pol α activity is competitive with respect to the DNA template primer (Ki=0.23 μM) and non-competitive with respect to the 2'-deoxyribonucleoside 5'-triphosphate substrate (Ki=0.18 μM) . Dehydroaltenusin arrests the cancer cell cycle at the S-phase and triggers apoptosis . Dehydroaltenusin possesses anti-tumor activity against human adenocarcinoma tumor in vivo .
BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm .
MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.
2× High-Fidelity PCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water.
2× PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water, in which bromophenol blue dye is included.
SYBR Green qPCR Master Mix (No ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which no ROX Reference Dye is included.
2× Fast PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for fast PCR except sample DNA, primers and water, in which bromophenol blue dye is included.
SYBR Green qPCR Master Mix (Low ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which Low ROX Reference Dye is included.
MCE 2× High-Fidelity PCR Master Mix (with Dye) is a new type of ready-to-use high-fidelity PCR amplification master mix.During the reaction, only template and primers need to be added to quickly and efficiently complete the PCR process, reducing the risk of contamination during operation.
T4 DNA polymerase has 5´→3´ DNA polymerase activity and can catalyze the synthesis of DNA along the 5´→3´ direction with the presence of
templates and primers.
SYBR Green qPCR Master Mix (High ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which High ROX Reference Dye is included.
Limochromin is a pigment produced by Streptomyces limosus that strongly inhibits the reverse transcriptase of avian myeloid leukemia virus (AMV). The inhibitory activity of limochromin is not affected by changes in the concentration of template-primer, substrate or carrier protein, but its inhibitory effect is significantly reduced when the concentration of AMV reverse transcriptase increases. Limochromin can be used to develop antiviral treatments .
Dehydroaltenusin is a small molecule selective inhibitor of eukaryotic DNA polymerase α, a type of antibiotic produced by a fungus with an IC50 value of 0.68 μM. The inhibitory mode of action of dehydroaltenusin against mammalian pol α activity is competitive with respect to the DNA template primer (Ki=0.23 μM) and non-competitive with respect to the 2'-deoxyribonucleoside 5'-triphosphate substrate (Ki=0.18 μM) . Dehydroaltenusin arrests the cancer cell cycle at the S-phase and triggers apoptosis . Dehydroaltenusin possesses anti-tumor activity against human adenocarcinoma tumor in vivo .
The PGK1 protein catalyzes the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate and plays a central role in ATP production.It also acts as a polymerase alpha cofactor protein and may be involved in primer recognition.PGK1 Protein, Mouse (sf9, His) is the recombinant mouse-derived PGK1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
The PGK1 protein plays a key role in the glycolysis pathway by catalyzing one of two ATP-generating reactions, promoting the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate. In addition to its glycolytic function, PGK1 appears to act as a cofactor protein for polymerase α, playing a role in primer recognition. PGK1 Protein, Human (HEK293, His) is the recombinant human-derived PGK1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PGK1 Protein, Human (HEK293, His) is 416 a.a., with molecular weight of 46-50 kDa.
