Silybin A (Synonyms: Silibinin A)

Name: Silybin A
Brand: MedChemExpress
SKU: HY-13748
Rating: 5.0 (12 reviews)

Cat. No.: HY-13748 Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

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Silybin A (Silibinin A), an effective anti-cancer and chemopreventive agent, has been shown to exert multiple effects on cancer cells, including inhibition of both cell proliferation and migration.

For research use only. We do not sell to patients.

Silybin A Chemical Structure

CAS No. : 22888-70-6

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 96	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 96	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 770	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Silybin A:

Isosilybin In-stock
Silibinin-d Get quote
Silibinin-d5 Get quote
Silybin Get quote
Silybin B Get quote
Silymarin Get quote
Silybin A (Standard) Get quote

10 Publications Citing Use of MCE Silybin A

IHC

: Silybin A purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Aug 14;11(8):630. [Abstract]; Silibinin (200 μM)-induced accumulation of AIF in nuclear fraction is decreased in the cells transfected with AIF SiRNA.

: Silybin A purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Aug 14;11(8):630. [Abstract]; Representative images acquired by confocal microscopy combined with immunochemical staining shows that Silibinin (200 μM) induced accumulation of AIF in the nucleus of U87 cell.

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Biological Activity
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Description

Silybin A (Silibinin A), an effective anti-cancer and chemopreventive agent, has been shown to exert multiple effects on cancer cells, including inhibition of both cell proliferation and migration.

Cellular Effect

Cell Line	Type	Value	Description	References
B16-4A5	IC₅₀	> 100 μM Compound: 5	Toxicity in mouse B16-4A5 cells assessed as inhibition of cell proliferation in presence of 1 mM theophylline after 72 hrs by WST8 dye reduction assay Toxicity in mouse B16-4A5 cells assessed as inhibition of cell proliferation in presence of 1 mM theophylline after 72 hrs by WST8 dye reduction assay	[PMID: 20189399]
B16-4A5	IC₅₀	21 μM Compound: 5	Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs	[PMID: 20189399]
HEK293	IC₅₀	2.7 μM Compound: Silybin A	Inhibition of OATP1B3 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting Inhibition of OATP1B3 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting	[PMID: 23401473]
HEK293	IC₅₀	9.7 μM Compound: Silybin A	Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting Inhibition of OATP1B1 (unknown origin) expressed in HEK293 cells assessed as reduction of [3H]estradiol-17beta-glucuronide uptake after 3 mins by beta-counting	[PMID: 23401473]
HepG2	EC₅₀	75.7 μM Compound: Silybin	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by SRB assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by SRB assay	[PMID: 29353722]
Huh-7	IC₅₀	80 μM Compound: 1	Cytotoxicity against human Huh7.5.1 cells after 72 hrs Cytotoxicity against human Huh7.5.1 cells after 72 hrs	[PMID: 23673225]
Huh7.5.1	CC₅₀	80 μM Compound: 3	Cytotoxicity against human Huh7.5.1 cells after 72 hrs by ATPlite assay Cytotoxicity against human Huh7.5.1 cells after 72 hrs by ATPlite assay	[PMID: 30485098]
HUVEC	IC₅₀	62.33 μM Compound: 1a, (2R,3R,10R,11R)	Cytotoxicity against HUVEC assessed as cell viability after 16 hrs by MTT assay Cytotoxicity against HUVEC assessed as cell viability after 16 hrs by MTT assay	[PMID: 21928794]
HUVEC	IC₅₀	8.83 μM Compound: 1a, (2R,3R,10R,11R)	Antiproliferative activity against HUVEC assessed as inhibition of cell growth after 3 days by MTT assay Antiproliferative activity against HUVEC assessed as inhibition of cell growth after 3 days by MTT assay	[PMID: 21928794]
LoVo	IC₅₀	50 μM Compound: Silybin A; Silibinin	Cytotoxicity against human LoVo cells assessed as reduction in cell growth after 24 hrs by trypan blue exclusion assay Cytotoxicity against human LoVo cells assessed as reduction in cell growth after 24 hrs by trypan blue exclusion assay	[PMID: 27517806]
LX-2	IC₅₀	151.1 μM Compound: Silybin	Cytotoxicity against human LX2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human LX2 cells assessed as reduction in cell viability by MTT assay	[PMID: 33775837]
MCF7	IC₅₀	222.8 μM Compound: 1	Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay Cytotoxicity in human MCF7 cells assessed as reduction in cell viability incubated for 68 hrs by MTT assay	[PMID: 28411546]
MDCK-II	IC₅₀	4.5 μM Compound: Silybin A	Inhibition of OATP2B1 (unknown origin) expressed in MDCK2 cells assessed as reduction of [3H]estrone-3-sulfate uptake after 3 mins by beta-counting Inhibition of OATP2B1 (unknown origin) expressed in MDCK2 cells assessed as reduction of [3H]estrone-3-sulfate uptake after 3 mins by beta-counting	[PMID: 23401473]
T47D	IC₅₀	110 μM Compound: Silybin A; Silibinin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27517806]
T47D	IC₅₀	12 μM Compound: Silybin A; Silibinin	Downregulation of telomerase expression in human T47D cells after 72 hrs in presence of curcumin by SYBR green-dye based RT-PCR analysis Downregulation of telomerase expression in human T47D cells after 72 hrs in presence of curcumin by SYBR green-dye based RT-PCR analysis	[PMID: 27517806]
T47D	IC₅₀	20 μM Compound: Silybin A; Silibinin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs in presence of curcumin by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs in presence of curcumin by MTT assay	[PMID: 27517806]

In Vitro

Silybin A (Silybin) significantly induced the expression of the non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) in both p53 wild-type and p53-null cancer cell lines^[1].
Silybin A (Silybin) induced cell death in human breast cancer cell lines MCF7 and MDA-MB-231^[2].
Silybin A (Silybin) treatment resulted in a dose- and time-dependent inhibition of HCC cell viability^[3].
Silybin A (Silybin) treatment decreased the expression of the Notch1 intracellular domain (NICD), RBP-Jκ, and Hes1 proteins, upregulated the apoptosis pathway-related protein Bax, and downregulated Bcl2, survivin, and cyclin D1^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Topical application of Silybin A (Silibinin A) at the dose of 9 mg/mouse effectively suppressed oxidative stress and deregulated activation of inflammatory mediators and tumorigenesis^[4].
The kidney cortex of vehicle-treated control OVE26 mice displayed greater Nox4 expression and twice as much superoxide production than cortex of silybin-treated mice. The glomeruli of control OVE26 mice displayed 35% podocyte drop out that was not present in the silybin-treated mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

482.44

Formula

C₂₅H₂₂O₁₀

CAS No.

22888-70-6

Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@H](O)[C@@H](C2=CC=C(O[C@H](CO)[C@@H](C3=CC=C(O)C(OC)=C3)O4)C4=C2)OC5=CC(O)=CC(O)=C15

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 233.33 mg/mL (483.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0728 mL	10.3640 mL	20.7280 mL
5 mM	0.4146 mL	2.0728 mL	4.1456 mL
10 mM	0.2073 mL	1.0364 mL	2.0728 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (248 KB) HNMR (264 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Woo SM, et al. Silibinin induces apoptosis of HT29 colon carcinoma cells through early growth response-1 (EGR-1)-mediated non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) up-regulation. Chem Biol Interact. 2014 Jan 16;211C:36-43. [Content Brief]

[2]. Kim TH, et al. Silibinin induces cell death through ROS-dependent down-regulation of Notch-1/ERK/Akt signaling in human breast cancer cells. J Pharmacol Exp Ther. 2014 Jan 28. [Content Brief]

[3]. Zhang S, et al. Silybin-mediated inhibition of Notch signaling exerts antitumor activity in human hepatocellular carcinoma cells. PLoS One. 2013 Dec 27;8(12):e83699. [Content Brief]

[4]. Khan AQ, et al. Silibinin Inhibits Tumor Promotional Triggers and Tumorigenesis Against Chemically Induced Two-Stage Skin Carcinogenesis in Swiss Albino Mice: Possible Role of Oxidative Stress and Inflammation. Nutr Cancer. 2013 Dec 23. [Content Brief]

[5]. Khazim K, et al. The antioxidant silybin prevents high glucose-induced oxidative stress and podocyte injury in vitro and in vivo. Am J Physiol Renal Physiol. 2013 Sep 1;305(5):F691-700. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0728 mL	10.3640 mL	20.7280 mL	51.8199 mL
	5 mM	0.4146 mL	2.0728 mL	4.1456 mL	10.3640 mL
	10 mM	0.2073 mL	1.0364 mL	2.0728 mL	5.1820 mL
	15 mM	0.1382 mL	0.6909 mL	1.3819 mL	3.4547 mL
	20 mM	0.1036 mL	0.5182 mL	1.0364 mL	2.5910 mL
	25 mM	0.0829 mL	0.4146 mL	0.8291 mL	2.0728 mL
	30 mM	0.0691 mL	0.3455 mL	0.6909 mL	1.7273 mL
	40 mM	0.0518 mL	0.2591 mL	0.5182 mL	1.2955 mL
	50 mM	0.0415 mL	0.2073 mL	0.4146 mL	1.0364 mL
	60 mM	0.0345 mL	0.1727 mL	0.3455 mL	0.8637 mL
	80 mM	0.0259 mL	0.1295 mL	0.2591 mL	0.6477 mL
	100 mM	0.0207 mL	0.1036 mL	0.2073 mL	0.5182 mL

Silybin A Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Silybin A22888-70-6Silibinin AAutophagyReactive Oxygen SpeciesInhibitorinhibitorinhibit

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Silybin A (Synonyms: Silibinin A)

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Other Forms of Silybin A:

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10 Publications Citing Use of MCE Silybin A

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Complete Stock Solution Preparation Table

Silybin A Related Classifications

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