Stavudine (Synonyms: d4T)

Name: Stavudine
Brand: MedChemExpress
SKU: HY-B0116
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0116 Purity: 99.87%: FAQs
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Stavudine (d4T) is an orally active nucleoside reverse transcriptase inhibitor (NRTI). Stavudine has activity against HIV-1 and HIV-2. Stavudine also inhibits the replication of mitochondrial DNA (mtDNA). Stavudine reduces NLRP3 inflammasome activation and modulates Amyloid-β autophagy. Stavudine induces apoptosis.

For research use only. We do not sell to patients.

Stavudine Chemical Structure

CAS No. : 3056-17-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Stavudine:

Stavudine-13C,d3 Get quote
Stavudine-d₄ Get quote
Stavudine sodium Get quote
Stavudine (Standard) Get quote

3 Publications Citing Use of MCE Stavudine

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NLRP3 NLRP1 NOD1 NOD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

HIV-1

HIV-2

NLRP3

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	> 250 μM Compound: 2, d4T	Cytostatic activity against human Caco2 cells assessed as growth inhibition after 3 days by particle counting analysis Cytostatic activity against human Caco2 cells assessed as growth inhibition after 3 days by particle counting analysis	[PMID: 24177359]
CCRF-CEM	IC₅₀	> 10 μM Compound: 1 (d4T)	Compound was tested for anti-HIV activity in TK-deficient CEM cells by microculture tetrazolium assay Compound was tested for anti-HIV activity in TK-deficient CEM cells by microculture tetrazolium assay	[PMID: 9873688]
CCRF-CEM	CC₅₀	> 100 μM Compound: Stavudine	Cytotoxic concentration of compound required to reduce the viability of mock infected CEM cells by 50% Cytotoxic concentration of compound required to reduce the viability of mock infected CEM cells by 50%	[PMID: 14980640]
CCRF-CEM	IC₅₀	> 250 μM Compound: 2, d4T	Cytostatic activity against thymidine kinase-deficient human CEM cells assessed as growth inhibition after 3 days by particle counting analysis Cytostatic activity against thymidine kinase-deficient human CEM cells assessed as growth inhibition after 3 days by particle counting analysis	[PMID: 24177359]
CCRF-CEM	CC₅₀	> 50 μM Compound: 1a; d4T	Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days	[PMID: 32515595]
CCRF-CEM	IC₅₀	≥ 250 μM Compound: 2, d4T	Cytostatic activity against human CEM cells assessed as growth inhibition after 3 days by particle counting analysis Cytostatic activity against human CEM cells assessed as growth inhibition after 3 days by particle counting analysis	[PMID: 24177359]
CCRF-CEM	EC₅₀	0.16 μM Compound: 1 (d4T)	Compound was evaluated for the inhibition of HIV replication using HIV-1 infected CEM cells. Compound was evaluated for the inhibition of HIV replication using HIV-1 infected CEM cells.	10.1016/0960-894X(96)00195-3
CCRF-CEM	EC₅₀	0.27 μM Compound: 1 (d4T)	Compound was evaluated for the inhibition of HIV replication using HIV-2 infected CEM cells. Compound was evaluated for the inhibition of HIV replication using HIV-2 infected CEM cells.	10.1016/0960-894X(96)00195-3
CCRF-CEM	EC₅₀	0.39 μM Compound: 2, d4T	Antiviral activity against HIV1 3B infected in human CEM cells assessed as virus-induced giant cell formation after 4 days by microscopic analysis Antiviral activity against HIV1 3B infected in human CEM cells assessed as virus-induced giant cell formation after 4 days by microscopic analysis	[PMID: 24177359]
CCRF-CEM	EC₅₀	0.56 μM Compound: 1, (d4T)	Antiviral activity against HIV1 in wild type human CEM cells assessed as virus-induced cytopathic effect after 4 to 5 days by microscopy Antiviral activity against HIV1 in wild type human CEM cells assessed as virus-induced cytopathic effect after 4 to 5 days by microscopy	[PMID: 19438207]
CCRF-CEM	EC₅₀	0.58 μM Compound: 2, d4T	Antiviral activity against HIV2 ROD infected in human CEM cells assessed as virus-induced giant cell formation after 4 days by microscopic analysis Antiviral activity against HIV2 ROD infected in human CEM cells assessed as virus-induced giant cell formation after 4 days by microscopic analysis	[PMID: 24177359]
CCRF-CEM	IC₅₀	0.6 μM Compound: 1 (d4T)	Compound was tested for anti-HIV activity in TK-deficient CEM cells by p24 production assay Compound was tested for anti-HIV activity in TK-deficient CEM cells by p24 production assay	[PMID: 9873688]
CCRF-CEM	EC₅₀	0.651 μM Compound: d4T	Concentration required to protect CEM cells against cytopathicity of HIV-1 by 50% Concentration required to protect CEM cells against cytopathicity of HIV-1 by 50%	[PMID: 10714505]
CCRF-CEM	EC₅₀	0.77 μM Compound: d4T	Concentration required to protect CEM cells against cytopathicity of HIV-2 by 50% Concentration required to protect CEM cells against cytopathicity of HIV-2 by 50%	[PMID: 10714505]
CCRF-CEM	EC₅₀	0.78 μM Compound: d4T, stavudine	Antiviral activity against HIV-1 infected in wild type CEM cells assessed as inhibition of virus-induced cytopathicity after 4 days Antiviral activity against HIV-1 infected in wild type CEM cells assessed as inhibition of virus-induced cytopathicity after 4 days	[PMID: 22827702]
CCRF-CEM	EC₅₀	0.79 μM Compound: 1, (d4T)	Antiviral activity against HIV2 in wild type human CEM cells assessed as virus-induced cytopathic effect after 4 to 5 days by microscopy Antiviral activity against HIV2 in wild type human CEM cells assessed as virus-induced cytopathic effect after 4 to 5 days by microscopy	[PMID: 19438207]
CCRF-CEM	EC₅₀	1.3 μM Compound: d4T, stavudine	Antiviral activity against HIV-2 infected in wild type CEM cells assessed as inhibition of virus-induced cytopathicity after 4 days Antiviral activity against HIV-2 infected in wild type CEM cells assessed as inhibition of virus-induced cytopathicity after 4 days	[PMID: 22827702]
CCRF-CEM	EC₅₀	150 μM Compound: d4T, stavudine	Antiviral activity against HIV-2 infected in thymidine kinase deficient CEM cells assessed as inhibition of virus-induced cytopathicity after 4 days Antiviral activity against HIV-2 infected in thymidine kinase deficient CEM cells assessed as inhibition of virus-induced cytopathicity after 4 days	[PMID: 22827702]
CCRF-CEM	CC₅₀	174 μM Compound: d4T	Compound was evaluated for the cytotoxicity against CEM cell proliferation by 50% Compound was evaluated for the cytotoxicity against CEM cell proliferation by 50%	[PMID: 10714505]
CCRF-CEM	CC₅₀	174 μM Compound: 1	Cytostatic concentration required to inhibit CEM/0 cells proliferation by 50% Cytostatic concentration required to inhibit CEM/0 cells proliferation by 50%	[PMID: 10514282]
CCRF-CEM	IC₅₀	2.4 μM Compound: 1 (d4T)	Compound was tested for anti-HIV activity in TK-deficient CEM cells by inhibition of reverse transcriptase activity Compound was tested for anti-HIV activity in TK-deficient CEM cells by inhibition of reverse transcriptase activity	[PMID: 9873688]
CCRF-CEM	EC₅₀	25 μM Compound: 1 (d4T)	Compound was evaluated for the inhibition of HIV replication using HIV-2 infected CEM-TK- cells. Compound was evaluated for the inhibition of HIV replication using HIV-2 infected CEM-TK- cells.	10.1016/0960-894X(96)00195-3
CCRF-CEM	EC₅₀	29 μM Compound: 1, (d4T)	Antiviral activity against HIV2 in thymidine kinase deficient human CEM cells assessed as virus-induced cytopathic effect after 4 to 5 days by microscopy Antiviral activity against HIV2 in thymidine kinase deficient human CEM cells assessed as virus-induced cytopathic effect after 4 to 5 days by microscopy	[PMID: 19438207]
CCRF-CEM	EC₅₀	31.05 μM Compound: 1; d4T	Antiviral activity against HIV2 ROD infected in thymidine kinase-deficient human CEM cells assessed as inhibition of virus-induced giant cell formation measured after 4 to 5 days by microscopic analysis Antiviral activity against HIV2 ROD infected in thymidine kinase-deficient human CEM cells assessed as inhibition of virus-induced giant cell formation measured after 4 to 5 days by microscopic analysis	[PMID: 32421343]
CCRF-CEM	EC₅₀	33 μM Compound: d4T	Concentration required to protect CEM/TK- cells against cytopathicity of HIV-2 by 50% Concentration required to protect CEM/TK- cells against cytopathicity of HIV-2 by 50%	[PMID: 10714505]
CCRF-CEM	CC₅₀	79 μM Compound: d4T	Cytotoxicity against human CEM/0 cells assessed as reduction in cell proliferation incubated for 4 to 5 days Cytotoxicity against human CEM/0 cells assessed as reduction in cell proliferation incubated for 4 to 5 days	[PMID: 26125628]
CCRF-CEM	CC₅₀	93 μM Compound: 1 (D4T)	Cytostatic concentration required to inhibit Human T-Lymphocyte (CEM) cells proliferation caused by HIV-1 (IIIB) Cytostatic concentration required to inhibit Human T-Lymphocyte (CEM) cells proliferation caused by HIV-1 (IIIB)	[PMID: 12852759]
CEM-c113	EC₅₀	0.08 μM Compound: D4T	Effective concentration that reduces HIV-induced cytopathic effect by 50% was determined by MTT assay. Effective concentration that reduces HIV-induced cytopathic effect by 50% was determined by MTT assay.	[PMID: 8385224]
COLO 320	IC₅₀	159 μM Compound: 2, d4T	Cytostatic activity against human COLO320 cells assessed as growth inhibition after 3 days by particle counting analysis Cytostatic activity against human COLO320 cells assessed as growth inhibition after 3 days by particle counting analysis	[PMID: 24177359]
H9	EC₅₀	0.06 μM Compound: D4T, NSC-163661	Antiviral activity against HIV1 3B in human H9 cells assessed as inhibition of virus-induced cytopathic effect by formazan-based conventional colorimetric technique Antiviral activity against HIV1 3B in human H9 cells assessed as inhibition of virus-induced cytopathic effect by formazan-based conventional colorimetric technique	[PMID: 11430019]
H9	EC₅₀	0.06 μM Compound: D4T, NSC-163661	Antiviral activity against HIV1 RF in human H9 cells assessed as inhibition of virus-induced cytopathic effect by formazan-based conventional colorimetric technique Antiviral activity against HIV1 RF in human H9 cells assessed as inhibition of virus-induced cytopathic effect by formazan-based conventional colorimetric technique	[PMID: 11430019]
HEK-293T	CC₅₀	> 100 μM Compound: d4T	Cytotoxicity against human 293T cells assessed as inhibition of cell proliferation by tetrazolium dye method Cytotoxicity against human 293T cells assessed as inhibition of cell proliferation by tetrazolium dye method	[PMID: 17470654]
HeLa	EC₅₀	> 200 μM Compound: d4T, Stavudine	Cytotoxicity against human HeLa P4/R5 cells by MTS assay Cytotoxicity against human HeLa P4/R5 cells by MTS assay	[PMID: 21382714]
HeLa	IC₅₀	> 250 μM Compound: 2, d4T	Cytostatic activity against thymidine kinase-deficient human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis Cytostatic activity against thymidine kinase-deficient human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis	[PMID: 24177359]
HeLa	IC₅₀	> 250 μM Compound: 2, d4T	Cytostatic activity against human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis Cytostatic activity against human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis	[PMID: 24177359]
L1210	IC₅₀	≥ 250 μM Compound: 2, d4T	Cytostatic activity against thymidine kinase-deficient mouse L1210 cells assessed as growth inhibition after 2 days by particle counting analysis Cytostatic activity against thymidine kinase-deficient mouse L1210 cells assessed as growth inhibition after 2 days by particle counting analysis	[PMID: 24177359]
L1210	IC₅₀	8.9 μM Compound: 2, d4T	Cytostatic activity against mouse L1210 cells assessed as growth inhibition after 2 days by particle counting analysis Cytostatic activity against mouse L1210 cells assessed as growth inhibition after 2 days by particle counting analysis	[PMID: 24177359]
MT2	IC₅₀	> 100 μM Compound: 1, d4T	Cytotoxicity against human MT2 cells after 5 days by MTT assay Cytotoxicity against human MT2 cells after 5 days by MTT assay	[PMID: 23380374]
MT2	CC₅₀	> 100 μM Compound: d4T	Cytotoxicity against human MT2 cells after 5 days by MTT assay Cytotoxicity against human MT2 cells after 5 days by MTT assay	[PMID: 20605472]
MT2	CC₅₀	> 100 μM Compound: d4T	Cytotoxicity against human MT2 cells by MTT assay Cytotoxicity against human MT2 cells by MTT assay	[PMID: 20304641]
MT2	CC₅₀	> 100 μM Compound: d4T	Cytotoxicity against human MT2 cells infected with HIV1 harboring reverse transcriptase Y181C mutation by MTT assay Cytotoxicity against human MT2 cells infected with HIV1 harboring reverse transcriptase Y181C mutation by MTT assay	[PMID: 20304641]
MT2	CC₅₀	> 100 μM Compound: d4T	Cytotoxicity against human MT2 cells infected with HIV1 harboring reverse transcriptase K103N/Y181C mutation by MTT assay Cytotoxicity against human MT2 cells infected with HIV1 harboring reverse transcriptase K103N/Y181C mutation by MTT assay	[PMID: 20304641]
MT2	CC₅₀	> 100 μM Compound: d4t	Cytotoxicity against human MT2 cells by MTT assay Cytotoxicity against human MT2 cells by MTT assay	[PMID: 17918923]
MT2	CC₅₀	> 100 μM Compound: d4T	Cytotoxicity against human MT2 cells by MTT assay Cytotoxicity against human MT2 cells by MTT assay	[PMID: 16298131]
MT2	EC₅₀	0.5 μM Compound: d4T	Antiviral activity against HIV1 reverse transcriptase K103N/Y181C double mutant infected in human MT2 cells assessed as inhibition of viral replication by MTT assay Antiviral activity against HIV1 reverse transcriptase K103N/Y181C double mutant infected in human MT2 cells assessed as inhibition of viral replication by MTT assay	[PMID: 20304641]
MT2	EC₅₀	0.7 μM Compound: d4T	Antiviral activity against HIV1 reverse transcriptase Y181C mutant infected in human MT2 cells assessed as inhibition of viral replication by MTT assay Antiviral activity against HIV1 reverse transcriptase Y181C mutant infected in human MT2 cells assessed as inhibition of viral replication by MTT assay	[PMID: 20304641]
MT2	EC₅₀	1.4 μM Compound: d4T	Antiviral activity against HIV1 3B infected in human MT2 cells assessed as inhibition of virus replication by MTT assay Antiviral activity against HIV1 3B infected in human MT2 cells assessed as inhibition of virus replication by MTT assay	[PMID: 20304641]
MT2	EC₅₀	1.6 μM Compound: d4t	Antiviral activity against HIV1 3B in human MT2 cells by MTT assay Antiviral activity against HIV1 3B in human MT2 cells by MTT assay	[PMID: 17918923]
MT2	CC₅₀	100 μM Compound: Stavudine	Cytotoxic concentration required to reduce the viability of mock-infected MT-2 cells by 50% Cytotoxic concentration required to reduce the viability of mock-infected MT-2 cells by 50%	[PMID: 14552777]
MT2	EC₅₀	2 μM Compound: d4T	Antiviral activity against HIV1 3B in MT2 cells assessed as inhibition of viral-mediated T-cell death by MTT method Antiviral activity against HIV1 3B in MT2 cells assessed as inhibition of viral-mediated T-cell death by MTT method	[PMID: 17317163]
MT2	CC₅₀	2.2 μM Compound: d4T	Cytotoxicity against human MT2 cells after 5 days by MTT assay Cytotoxicity against human MT2 cells after 5 days by MTT assay	[PMID: 24900627]
MT2	EC₅₀	2.2 μM Compound: 1, d4T	Antiviral activity against HIV-1 infected un MT2 cells assessed as virus-induced cytopathicity after 5 days by MTT assay Antiviral activity against HIV-1 infected un MT2 cells assessed as virus-induced cytopathicity after 5 days by MTT assay	[PMID: 23380374]
MT2	EC₅₀	2.3 μM Compound: d4T	Antiviral activity against HIV1 3B infected in human MT-2 cells assessed as inhibition of viral-mediated cell death after 5 days by MTT assay Antiviral activity against HIV1 3B infected in human MT-2 cells assessed as inhibition of viral-mediated cell death after 5 days by MTT assay	[PMID: 20605472]
MT2	EC₅₀	3 μM Compound: d4T	Antiviral activity against HIV1 3B in MT2 cells by MTT assay Antiviral activity against HIV1 3B in MT2 cells by MTT assay	[PMID: 16298131]
MT2	EC₅₀	3.6 μM Compound: d4T	Antiviral activity against HIV1 3B infected in human MT2 cells after 5 days by MTT assay Antiviral activity against HIV1 3B infected in human MT2 cells after 5 days by MTT assay	[PMID: 24900627]
MT2	EC₅₀	4.8 μM Compound: 5	Inhibition of virus-induced cytopathic effect in wild type HIV 3a infected MT2 cells after 5 days Inhibition of virus-induced cytopathic effect in wild type HIV 3a infected MT2 cells after 5 days	[PMID: 17562366]
MT4	EC₅₀	0.51 μM Compound: Stavudine	Antiviral activity against HIV1 3B in MT4 cells by MTT assay Antiviral activity against HIV1 3B in MT4 cells by MTT assay	[PMID: 17181169]
MT4	IC₅₀	1.2 μM Compound: 1	Concentration required for the inhibition of HIV replication by 50% in MT-4 cells Concentration required for the inhibition of HIV replication by 50% in MT-4 cells	10.1016/S0960-894X(00)80147-X
SUP-T1	EC₅₀	136.1 μM Compound: d4T, Stavudine	Antiviral activity against Human immunodeficiency virus 1 3B infected in human SupT1 cells by cell-associated transmission assay Antiviral activity against Human immunodeficiency virus 1 3B infected in human SupT1 cells by cell-associated transmission assay	[PMID: 21382714]
WI-38	CC₅₀	> 100 μM Compound: Stavudine	Cytotoxicity against human WI38 cells by neutral red assay Cytotoxicity against human WI38 cells by neutral red assay	[PMID: 18285481]
WI-38	EC₅₀	> 100 μM Compound: Stavudine	Antimicrobial activity against BK polyomavirus ATCC VR837 infected in human WI38 cells assessed as reduction in viral titer after 7 days by PCR analysis Antimicrobial activity against BK polyomavirus ATCC VR837 infected in human WI38 cells assessed as reduction in viral titer after 7 days by PCR analysis	[PMID: 18285481]

In Vitro

Stavudine (d4T) (50 μM) significantly reduces the expression of the NLRP3 inflammasome gene, IL-18 production and Aβ 42-stimulated cellular production of IL-1β in THP-1 derived macrophages^[3].
? Stavudine (d4T) (50 μM) inhibits the assembly of the NLRP3 inflammasome complex and down-regulates the phagocytosis of Aβ 42 by macrophages^[3].
? Stavudine (d4T) (10 μM, 7 or 14 days) significantly induced CEM cells apoptosis, especially after 14 days, and increases hydrogen peroxide levels^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Stavudine (d4T) (500 mg/kg, daily liquid, 2 weeks) can rapidly induce fat depletion and mild liver damage at high dose in male RjOrl Swiss mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male RjOrl Swiss mice weighing 0.028-0.03 kg^[1]
Dosage:	500 mg/kg
Administration:	Daily liquid; 2 weeks
Result:	Reduced fat weight gain by 58%, 5.7 g in the control group and 4.9 g in the d4T treated group. Significantly elevated plasma ALT and LDH levels. Reduced plasma acetoacetic acid and beta-hydroxybutyric acid levels. Reduced liver and muscle mtDNA levels at high dose concentration of 500 mg/kg.

Clinical Trial

Molecular Weight

224.21

Formula

C₁₀H₁₂N₂O₄

CAS No.

3056-17-5

Appearance

Solid

Color

White to light yellow

SMILES

OC[C@H]1O[C@@H](N2C=C(C)C(NC2=O)=O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (557.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 16.67 mg/mL (74.35 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4601 mL	22.3005 mL	44.6010 mL
5 mM	0.8920 mL	4.4601 mL	8.9202 mL
10 mM	0.4460 mL	2.2301 mL	4.4601 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 120 mg/mL (535.21 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (281 KB) SDS (418 KB)

COA (252 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Moyle GJ, et al. The role of stavudine in the management of adults with HIV infection. Antivir Ther. 1997;2(4):207-218. [Content Brief]

[2]. Riddler SA, et al. Antiretroviral activity of stavudine (2',3'-didehydro-3'-deoxythymidine, D4T). Antiviral Res. 1995;27(3):189-203. [Content Brief]

[3]. La Rosa F, et al. Stavudine Reduces NLRP3 Inflammasome Activation and Modulates Amyloid-β Autophagy. J Alzheimers Dis. 2019;72(2):401-412. [Content Brief]

[4]. Ferraresi R, et al. Protective effect of acetyl-L-carnitine against oxidative stress induced by antiretroviral drugs. FEBS Lett. 2006;580(28-29):6612-6616. [Content Brief]

[5]. Anissa Igoudjil, et al. High doses of stavudine induce fat wasting and mild liver damage without impairing mitochondrial respiration in mice. Antivir Ther. 2007;12(3):389-400. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.4601 mL	22.3005 mL	44.6010 mL	111.5026 mL
	5 mM	0.8920 mL	4.4601 mL	8.9202 mL	22.3005 mL
	10 mM	0.4460 mL	2.2301 mL	4.4601 mL	11.1503 mL
	15 mM	0.2973 mL	1.4867 mL	2.9734 mL	7.4335 mL
	20 mM	0.2230 mL	1.1150 mL	2.2301 mL	5.5751 mL
	25 mM	0.1784 mL	0.8920 mL	1.7840 mL	4.4601 mL
	30 mM	0.1487 mL	0.7434 mL	1.4867 mL	3.7168 mL
	40 mM	0.1115 mL	0.5575 mL	1.1150 mL	2.7876 mL
	50 mM	0.0892 mL	0.4460 mL	0.8920 mL	2.2301 mL
	60 mM	0.0743 mL	0.3717 mL	0.7434 mL	1.8584 mL
DMSO	80 mM	0.0558 mL	0.2788 mL	0.5575 mL	1.3938 mL
DMSO	100 mM	0.0446 mL	0.2230 mL	0.4460 mL	1.1150 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Stavudine Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Stavudine3056-17-5d4TReverse TranscriptaseHIVNucleoside Antimetabolite/AnalogNOD-like Receptor (NLR)AutophagyApoptosisHuman immunodeficiency virusantiretroviralnucleosideanaloguemtDNAinflammasomeAmyloid-βInhibitorinhibitorinhibit

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Stavudine (Synonyms: d4T)

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Stavudine Related Antibodies

3 Publications Citing Use of MCE Stavudine

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Stavudine Related Antibodies

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