2. Neuronal Signaling Stem Cell/Wnt Apoptosis
  3. Notch Apoptosis
  4. Withanolide A

Withanolide A is an orally active extract from the Indian herb Ashwagandha. Withanolide A can induce apoptosis. Withanolide A has anti-inflammatory and antitumor activity. Withanolide A can be used in the study of neurodegenerative diseases.

Withanolide A is an orally active extract from the Indian herb Ashwagandha. Withanolide A can induce apoptosis. Withanolide A has anti-inflammatory and antitumor activity. Withanolide A can be used in the study of neurodegenerative diseases.

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Withanolide A Chemical Structure

Withanolide A Chemical Structure

CAS No. : 32911-62-9

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Based on 1 publication(s) in Google Scholar

Withanolide A Related Antibodies

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Description

Withanolide A is an orally active extract from the Indian herb Ashwagandha. Withanolide A can induce apoptosis. Withanolide A has anti-inflammatory and antitumor activity. Withanolide A can be used in the study of neurodegenerative diseases[1][2][3][4].

In Vitro

Withanolide A (1 μM, 4 days) can induce presynaptic and postsynaptic reconstruction of rat neurons and induce significant regeneration of axons and dendrites[1].
Withanolide A (0.5-2 μM, 24-48 h) inhibits growth of CaOV3 and SKOV3 ovarian cancer cells by targeting Notch1 and Notch3[2].
Withanolide A (0.5-50 μg/mL, 3 h) has the potential of vascular relaxation by promoting the production of NO in endothelial cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Withanolide A Related Antibodies

Cell Viability Assay[2]

Cell Line: CaOV3、SKOV3、OVCAR3、TOV112D and TOV21G
Concentration: 0, 0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10 μM
Incubation Time: 72 h
Result: Decreased the cell viability and with an IC50 concentrations at 520 nM, 627 nM, 452 nM, 262 nM, 243 nM, respectively.

Apoptosis Analysis[2]

Cell Line: CaOV3, SKOV3
Concentration: 0.5, 1, 2 μM
Incubation Time: 24, 48 h
Result: Induced apoptosis in a dose-dependent manner.

Cell Cycle Analysis[2]

Cell Line: CaOV3, SKOV3
Concentration: 0.5, 1, 2 μM
Incubation Time: 24, 48 h
Result: Induced G2/M phase cell cycle arrest.

Western Blot Analysis[2]

Cell Line: CaOV3, SKOV3
Concentration: 0.5, 1, 2 μM
Incubation Time: 24, 48 h
Result: Decreased cdc25C protein.
Cyclin B1 stayed unchanged at 24 h but decreased by 48 h in a dose-dependent manner in CaOV3 cells, transiently increased in SKOV3 cells.
Reduced the levels of phospho-Akt (ser 473) and Bcl-2.
Reduced Notch 3 protein levels in CaOV3 cells and Notch1 protein levels in SKOV3 cells.
In Vivo

Withanolide A (10 μmol/kg, dissolved in 0.5% acacia solution for 21 days orally) prevents neurodegeneration in rats by modulating hippocampal glutathione biosynthesis[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male Sprague Dawley rats weighing 240–250 g[4]
Dosage: 10 μmol/kg
Administration: p.o. (dissolved in 0.5% gum arabic solution)
Result: Decreased the level of reactive oxygen species generation and lipid peroxidation.
Increased the level of GSH and the activity of glutathione reductase, glutathione s transferase and superoxide dismutase.
Increased the ATP, NADPH and GCLC activity.
Decreased corticosterone level and glucocorticoid receptor.
Molecular Weight

470.60

Formula

C28H38O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1O[C@@]([H])([C@@](C)([C@H]2CC[C@]3([C@@]4([C@H]5[C@@H]([C@@](O)(CC=CC6=O)[C@]6([C@]4(CC[C@@]32C)[H])C)O5)[H])[H])O)CC(C)=C1C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (21.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1249 mL 10.6247 mL 21.2495 mL
5 mM 0.4250 mL 2.1249 mL 4.2499 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1 mg/mL (2.12 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1 mg/mL (2.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1249 mL 10.6247 mL 21.2495 mL 53.1237 mL
5 mM 0.4250 mL 2.1249 mL 4.2499 mL 10.6247 mL
10 mM 0.2125 mL 1.0625 mL 2.1249 mL 5.3124 mL
15 mM 0.1417 mL 0.7083 mL 1.4166 mL 3.5416 mL
20 mM 0.1062 mL 0.5312 mL 1.0625 mL 2.6562 mL
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Withanolide A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Withanolide A32911-62-9NotchApoptosisAnti-inflammatoryCaOV3SKOV3Ea.hy926Inhibitorinhibitorinhibit

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