2. Metabolic Enzyme/Protease
  3. Mitochondrial Metabolism
  4. 2-Thenoyltrifluoroacetone

2-Thenoyltrifluoroacetone  (Synonyms: HTTA; TTA; TTFA)

Cat. No.: HY-D0190 Purity: 99.96%
SDS COA Handling Instructions

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2-Theoyltrifluoroacetone is a chelating agent and mitochondrial electron transfer chain inhibitor, with a IC50 value of 51.5 μM. 2-Thermoyltrifluoroacetone can chelate with various metal ions and has cytotoxicity and anti-tumor activity, which is expected to play an important role in the treatment of mitochondrial dysfunction related diseases[1][2][3].

For research use only. We do not sell to patients.

2-Thenoyltrifluoroacetone Chemical Structure

2-Thenoyltrifluoroacetone Chemical Structure

CAS No. : 326-91-0

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Based on 2 publication(s) in Google Scholar

2-Thenoyltrifluoroacetone Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

2-Theoyltrifluoroacetone is a chelating agent and mitochondrial electron transfer chain inhibitor, with a IC50 value of 51.5 μM. 2-Thermoyltrifluoroacetone can chelate with various metal ions and has cytotoxicity and anti-tumor activity, which is expected to play an important role in the treatment of mitochondrial dysfunction related diseases[1][2][3].
IC50 & Target

IC50: 51.5 μM (mitochondrial electron transport chain)[1].
IC50: 0.54 μM (carboxylesterase)[4].
In Vitro

2-Thenoyltrifluoroacetone (100-1000μM, 24 h) increases mitochondrial ROS production and induces microglial cell activation by inhibiting mitochondrial electron transport chain[1].
2-Thenoyltrifluoroacetone (28.2-51.5 μM, 72 h) exhibits cytotoxicity and anticancer activity on K562 cells by forming a complex with copper (II) [2].
2-Thenoyltrifluoroacetone (500, 750, 1000 ng/mL, 24, 48, 72 h) exhibits cytotoxicity and anti-tumor activity against MCF-7 and MKN-45 cells by forming a complex with zinc (II) [3].
2-Thenoyltrifluoroacetone (1-25 μM, 20 min) significantly inhibits carboxylesterase activity in rat plasma and mitochondria [4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Thenoyltrifluoroacetone Related Antibodies

Cell Viability Assay[1].

Cell Line: microglial cell
Concentration: 100-1000 μM
Incubation Time: 24 h
Result: Activated microglial cell within a certain concentration range [1].

Cell Cytotoxicity Assay[2].

Cell Line: K562 cell
Concentration: 28.2-51.5 μM
Incubation Time: 72 h
Result: Improved the cytotoxic activity in the K562 cells[2].

Cell Cytotoxicity Assay[3].

Cell Line: MCF-7 cell, MKN-45 cell
Concentration: 500, 750, 1000 ng/mL
Incubation Time: 24, 48, 72 h
Result: Improved the cytotoxic activity in the MCF-7 and MKN-45 cells
Molecular Weight

222.18

Formula

C8H5F3O2S
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=CC=CS1)CC(C(F)(F)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (450.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5009 mL 22.5043 mL 45.0086 mL
5 mM 0.9002 mL 4.5009 mL 9.0017 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.96%

SDS (393 KB)

COA (248 KB) HNMR (113 KB) GC (179 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5009 mL 22.5043 mL 45.0086 mL 112.5214 mL
5 mM 0.9002 mL 4.5009 mL 9.0017 mL 22.5043 mL
10 mM 0.4501 mL 2.2504 mL 4.5009 mL 11.2521 mL
15 mM 0.3001 mL 1.5003 mL 3.0006 mL 7.5014 mL
20 mM 0.2250 mL 1.1252 mL 2.2504 mL 5.6261 mL
25 mM 0.1800 mL 0.9002 mL 1.8003 mL 4.5009 mL
30 mM 0.1500 mL 0.7501 mL 1.5003 mL 3.7507 mL
40 mM 0.1125 mL 0.5626 mL 1.1252 mL 2.8130 mL
50 mM 0.0900 mL 0.4501 mL 0.9002 mL 2.2504 mL
60 mM 0.0750 mL 0.3751 mL 0.7501 mL 1.8754 mL
80 mM 0.0563 mL 0.2813 mL 0.5626 mL 1.4065 mL
100 mM 0.0450 mL 0.2250 mL 0.4501 mL 1.1252 mL
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2-Thenoyltrifluoroacetone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Thenoyltrifluoroacetone326-91-0HTTA TTA TTFAMitochondrial Metabolismcarboxyesterasecytotoxicitymicroglial cellK562 cellMCF-7 cellMKN-45 cellInhibitorinhibitorinhibit

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