2'-Deoxycytidine (Synonyms: Deoxycytidine; Cytosine deoxyriboside; Deoxyribose cytidine)

Name: 2'-Deoxycytidine
Brand: MedChemExpress
SKU: HY-D0184
Rating: 5.0 (5 reviews)

Cat. No.: HY-D0184 Purity: 99.95%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2'-Deoxycytidine, a deoxyribonucleoside, can inhibit biological effects of Bromodeoxyuridine (Brdu). 2'-Deoxycytidine is essential for the synthesis of nucleic acids, that can be used for the research of cancer.

For research use only. We do not sell to patients.

2'-Deoxycytidine Chemical Structure

CAS No. : 951-77-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 2'-Deoxycytidine:

5 Publications Citing Use of MCE 2'-Deoxycytidine

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Brdu

Human Endogenous Metabolite

Microbial Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
C6	IC₅₀	> 250 μM Compound: 2'-Deoxycytidine	Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
DU-145	IC₅₀	> 250 μM Compound: 2'-Deoxycytidine	Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
HaCaT	IC₅₀	> 250 μM Compound: 2'-Deoxycytidine	Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
HCT-116	EC₅₀	> 10 μM Compound: 5azadC	Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay Antiproliferative activity against human HCT116 cells after 2 to 4 days by ATPlite 1step luminescence assay	[PMID: 26220519]
KARPAS-299	EC₅₀	> 0 μM Compound: 5azadC	Antiproliferative activity against human KARPAS299 cells after 2 to 4 days by ATPlite 1step luminescence assay Antiproliferative activity against human KARPAS299 cells after 2 to 4 days by ATPlite 1step luminescence assay	[PMID: 26220519]
KG-1	EC₅₀	0.1 μM Compound: 5azadC	Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay Antiproliferative activity against human KG1 cells after 2 to 4 days by ATPlite 1step luminescence assay	[PMID: 26220519]
L6	IC₅₀	> 250 μM Compound: 2'-Deoxycytidine	Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
MCF7	IC₅₀	> 250 μM Compound: 2'-Deoxycytidine	Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H

In Vivo

2'-Deoxycytidine (25 mg/kg, i.v., 4 times, from 8 days to 16 days) inhibits tumor growth in mice inoculated SP2/0 myeloma cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice inoculated SP2/0 myeloma cells^[2]
Dosage:	25 mg/kg
Administration:	i.v., 4 times, from 8 days to 16 days
Result:	Decreased the tumor weight and volume. Increased survival time.

Clinical Trial

Molecular Weight

227.22

Formula

C₉H₁₃N₃O₄

CAS No.

951-77-9

Appearance

Solid

Color

White to off-white

SMILES

O=C1N([C@H]2C[C@H](O)[C@@H](CO)O2)C=CC(N)=N1

Structure Classification

Others

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (220.05 mM; Need ultrasonic)

DMSO : 50 mg/mL (220.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4010 mL	22.0051 mL	44.0102 mL
5 mM	0.8802 mL	4.4010 mL	8.8020 mL
10 mM	0.4401 mL	2.2005 mL	4.4010 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 110 mg/mL (484.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) RP-HPLC (244 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Horn D, et al. Inhibition of biological effects of bromodeoxyuridine by deoxycytidine: correlation with decreased incorporation of bromodeoxyuridine into DNA. Somatic Cell Genet. 1976 Sep;2(5):469-81. [Content Brief]

[2]. Iwazaki A, et al. Intravenously administered 2'-deoxycytidine suppresses mouse myeloma tumor growth. Biol Pharm Bull. 2012;35(2):251-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.4010 mL	22.0051 mL	44.0102 mL	110.0255 mL
	5 mM	0.8802 mL	4.4010 mL	8.8020 mL	22.0051 mL
	10 mM	0.4401 mL	2.2005 mL	4.4010 mL	11.0026 mL
	15 mM	0.2934 mL	1.4670 mL	2.9340 mL	7.3350 mL
	20 mM	0.2201 mL	1.1003 mL	2.2005 mL	5.5013 mL
	25 mM	0.1760 mL	0.8802 mL	1.7604 mL	4.4010 mL
	30 mM	0.1467 mL	0.7335 mL	1.4670 mL	3.6675 mL
	40 mM	0.1100 mL	0.5501 mL	1.1003 mL	2.7506 mL
	50 mM	0.0880 mL	0.4401 mL	0.8802 mL	2.2005 mL
	60 mM	0.0734 mL	0.3668 mL	0.7335 mL	1.8338 mL
	80 mM	0.0550 mL	0.2751 mL	0.5501 mL	1.3753 mL
	100 mM	0.0440 mL	0.2201 mL	0.4401 mL	1.1003 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2'-Deoxycytidine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2'-Deoxycytidine951-77-9Deoxycytidine Cytosine deoxyriboside Deoxyribose cytidineEndogenous Metabolitedeoxyribonucleosidecancernucleic acidsBrduInhibitorinhibitorinhibit

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2'-Deoxycytidine (Synonyms: Deoxycytidine; Cytosine deoxyriboside; Deoxyribose cytidine)

Customer Review

Other Forms of 2'-Deoxycytidine:

2'-Deoxycytidine Related Antibodies

5 Publications Citing Use of MCE 2'-Deoxycytidine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

2'-Deoxycytidine Related Classifications

Keywords:

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