2. Metabolic Enzyme/Protease NF-κB MAPK/ERK Pathway
  3. Aldehyde Dehydrogenase (ALDH) NF-κB p38 MAPK
  4. 3-Hydroxybenzaldehyde

3-Hydroxybenzaldehyde  (Synonyms: M-Hydroxybenzaldehyde)

Cat. No.: HY-76006 Purity: 99.84%
SDS COA Handling Instructions

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3-Hydroxybenzaldehyde (3-HBA) is a precursor compound for phenolic compounds like Protocatechuic aldehyde (PCA) (HY-N0295). 3-Hydroxybenzaldehyde, produced by 3-hydroxybenzyl-alcohol dehydrogenase, is a substrate of aldehyde dehydrogenase (ALDH) in rats and humans. 3-Hydroxybenzaldehyde has vasculoprotective effects in vitro and in vivo. 3-Hydroxybenzaldehyde is proming for research of atherosclerosis.

For research use only. We do not sell to patients.

3-Hydroxybenzaldehyde Chemical Structure

3-Hydroxybenzaldehyde Chemical Structure

CAS No. : 100-83-4

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

3-Hydroxybenzaldehyde Related Antibodies

Top Publications Citing Use of Products

View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

View All Isoforms
ALDH1 ALDH2 ALDH3

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK p38α p38β MAPK13 p38δ p38γ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

3-Hydroxybenzaldehyde (3-HBA) is a precursor compound for phenolic compounds like Protocatechuic aldehyde (PCA) (HY-N0295). 3-Hydroxybenzaldehyde, produced by 3-hydroxybenzyl-alcohol dehydrogenase, is a substrate of aldehyde dehydrogenase (ALDH) in rats and humans. 3-Hydroxybenzaldehyde has vasculoprotective effects in vitro and in vivo. 3-Hydroxybenzaldehyde is proming for research of atherosclerosis[1][2][3][4].

IC50 & Target

ALDH2

 

Cellular Effect
Cell Line Type Value Description References
MDA-MB-231 IC50
8.2 μM
Compound: 21
Growth inhibition of human MDA-MB-231 cells after 72 hrs by alamar blue assay
Growth inhibition of human MDA-MB-231 cells after 72 hrs by alamar blue assay
[PMID: 28001067]
In Vitro

3-Hydroxybenzaldehyde (0-100 μM, 24 h) inhibits vascular smooth muscle cells (VSMC) proliferation, cell cycle and cell migration[1].
3-Hydroxybenzaldehyde (100 μM, 25 h) inhibits production levels of p-NF-κB and p-p38 increased by TNF-α in human umbilical vein endothelial cells (HUVECs)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Hydroxybenzaldehyde Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Rat vascular smooth muscle cells (VSMCs)
Concentration: 0, 25, 50, 100 μM
Incubation Time: 48 h or 144 h
Result: Showed low toxicity in VSMC for both 48 and 144 h.

Cell Cycle Analysis[1]

Cell Line: Rat vascular smooth muscle cells (VSMCs)
Concentration: 100 μM
Incubation Time: 24 h
Result: Arrested the S phase and G0/G1 phase increased by PDGF and lowered the expression levels of cyclin D1 (CD1) and retinoblastoma (Rb1) mRNA in VSMCs.
In Vivo

3-Hydroxybenzaldehyde (100 mg/kg, i.p., daily for 1 week) inhibits angiogenesis and exhibits anti-thrombic effects ex vivo and in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6-week-old male SD rats[1]
Dosage: 100 mg/kg
Administration: i.p., daily for 1 week
Result: Exhibited anti-thrombic effects in vivo.
Molecular Weight

122.12

Formula

C7H6O2
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=CC1=CC=CC(O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (818.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.1887 mL 40.9433 mL 81.8867 mL
5 mM 1.6377 mL 8.1887 mL 16.3773 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.85%

SDS (393 KB)

COA (246 KB) HNMR (128 KB) GC (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 8.1887 mL 40.9433 mL 81.8867 mL 204.7167 mL
5 mM 1.6377 mL 8.1887 mL 16.3773 mL 40.9433 mL
10 mM 0.8189 mL 4.0943 mL 8.1887 mL 20.4717 mL
15 mM 0.5459 mL 2.7296 mL 5.4591 mL 13.6478 mL
20 mM 0.4094 mL 2.0472 mL 4.0943 mL 10.2358 mL
25 mM 0.3275 mL 1.6377 mL 3.2755 mL 8.1887 mL
30 mM 0.2730 mL 1.3648 mL 2.7296 mL 6.8239 mL
40 mM 0.2047 mL 1.0236 mL 2.0472 mL 5.1179 mL
50 mM 0.1638 mL 0.8189 mL 1.6377 mL 4.0943 mL
60 mM 0.1365 mL 0.6824 mL 1.3648 mL 3.4119 mL
80 mM 0.1024 mL 0.5118 mL 1.0236 mL 2.5590 mL
100 mM 0.0819 mL 0.4094 mL 0.8189 mL 2.0472 mL
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3-Hydroxybenzaldehyde Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Hydroxybenzaldehyde100-83-4M-HydroxybenzaldehydeAldehyde Dehydrogenase (ALDH)NF-κBp38 MAPKNuclear factor-κBNuclear factor-kappaBsubstratealdehydedehydrogenaseALDHVSMCHUVECscarotidballooninjuryphenolic compounds3-hydroxybenzyl-alcohol dehydrogenase vasculoprotective effects atherosclerosisInhibitorinhibitorinhibit

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