Arctigenin (Synonyms: (-)-Arctigenin)

Name: Arctigenin
Brand: MedChemExpress
SKU: HY-N0035
Rating: 5.0 (10 reviews)

Cat. No.: HY-N0035 Purity: 99.69%: FAQs
Data Sheet SDS COA Handling Instructions

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Arctigenin ((-)-Arctigenin), a biologically active lignan, can be used as an antitumor agent. Arctigenin exhibits potent antioxidant, anti-inflammatory and antiviral (influenza A virus) activities. Arctigenin can be used for the research of metabolic disorders, and central nervous system dysfunctions.

For research use only. We do not sell to patients.

Arctigenin Chemical Structure

CAS No. : 7770-78-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Arctigenin:

Arctigenin (Standard) Get quote

8 Publications Citing Use of MCE Arctigenin

RT-PCR

: Arctigenin purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2020 May;155:104721. [Abstract]; The Western blotting results show that incubating LA795 cells with 100 μM arctigenin led to a decrease in phospho-MEK1/2, phospho-ERK1/2 and cyclin D1 expression (n = 3).

: Arctigenin purchased from MedChemExpress. Usage Cited in: Life Sci. 2020 Sep 1;256:117983. [Abstract]; The indicated cell lines are treated with 5 μM Arc (Arctigenin) for 10 h and then harvested for extraction of total cellular RNA and subsequent qRT-PCR.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: ATG	Cytotoxicity against human A549 cells after 4 days under normal conditions by MTT assay Cytotoxicity against human A549 cells after 4 days under normal conditions by MTT assay	[PMID: 25571795]
A549	IC₅₀	0.8 μg/mL Compound: 9	Antiproliferative activity against human A549 cells assessed as inhibition of cell viability by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell viability by MTT assay	[PMID: 32223924]
A549	IC₅₀	6.57 μM Compound: ATG	Cytotoxicity against human A549 cells after 4 days under glucose-starved conditions by MTT assay Cytotoxicity against human A549 cells after 4 days under glucose-starved conditions by MTT assay	[PMID: 25571795]
HeLa	EC₅₀	> 100 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by WST-8 assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by WST-8 assay	[PMID: 28754364]
HeLa	IC₅₀	572.7 μM Compound: Arctigenin	Cytotoxicity against human HeLa cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability by MTT assay	[PMID: 30237124]
HepG2	IC₅₀	2.8 μg/mL Compound: 9	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability by MTT assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability by MTT assay	[PMID: 32223924]
HL-60	EC₅₀	12 μM Compound: 1	Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 48 hrs by WST-8 assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability after 48 hrs by WST-8 assay	[PMID: 28754364]
MDA-MB-231	IC₅₀	1.1 μg/mL Compound: 9	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability by MTT assay	[PMID: 32223924]
MRC5	IC₅₀	2.1 μM Compound: 7	Antiviral activity against HCMV in MRC5 cells by plaque reduction assay Antiviral activity against HCMV in MRC5 cells by plaque reduction assay	[PMID: 17239594]
PANC-1	IC₅₀	2.2 μM Compound: Arctigenin	Growth inhibition of human PANC1 cells in nutrient-deprived medium incubated for 24 hrs by MTT assay Growth inhibition of human PANC1 cells in nutrient-deprived medium incubated for 24 hrs by MTT assay	[PMID: 30878830]
RAW264.7	IC₅₀	0.26 mM Compound: 10	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 18986199]

In Vitro

Arctigenin (0.01-1 μg/mL; 0.5-24 h) shows preferential cytotoxicity against the PANC-1 cell line under nutrient-deprived conditions in a concentration- and time-dependent manner^[1].
? Arctigenin (0.01-1 μg/mL; 0.5-24 h) inhibits the survival of various cancer cell lines (ASPC-1, BXPC-3, Alexander, and PSN-1 cells) under nutrient-deprived conditions^[1].
? Arctigenin (0.1-1 μg/mL; 24 h) completely inhibits Akt phosphorylation stimulated by glucose deprivation in PANC-1 cells^[1].
? Arctigenin (5-25 μM) shows potent in vitro antiviral activities (IC₅₀=2.9 μM) against influenza A virus^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Arctigenin (250 μg/mL; 0.2 mL; i.p. 6 times a week for 9 weeks) suppress the PANC-1 tumor growth in nude mice^[1].
? Arctigenin (10 mg/kg; i.p. daily for 30 days) relieves experimental autoimmune encephalomyelitis (EAE) symptoms of mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female SPF/VAF BALB/cAn Ncrj-nu/nu mice were injected with PANC-1 cells^[1]
Dosage:	250 μg/mL, 0.2 mL
Administration:	I.p. 6 times a week for 9 weeks
Result:	Reduced the tumor weight and volume. No significant body weight loss was recognized.

Clinical Trial

Molecular Weight

372.41

Formula

C₂₁H₂₄O₆

CAS No.

7770-78-7

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC[C@H](CC2=CC=C(OC)C(OC)=C2)[C@H]1CC3=CC=C(O)C(OC)=C3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (268.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6852 mL	13.4261 mL	26.8521 mL
5 mM	0.5370 mL	2.6852 mL	5.3704 mL
10 mM	0.2685 mL	1.3426 mL	2.6852 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (250 KB) LCMS (309 KB)

Handling Instructions (2659 KB)

References

[1]. Awale S, et, al. Identification of arctigenin as an antitumor agent having the ability to eliminate the tolerance of cancer cells to nutrient starvation. Cancer Res. 2006 Feb 1;66(3):1751-7. [Content Brief]

[2]. Gao Q, et, al. Overview of the anti-inflammatory effects, pharmacokinetic properties and clinical efficacies of arctigenin and arctiin from Arctium lappa L. Acta Pharmacol Sin. 2018 May;39(5):787-801. [Content Brief]

[3]. Hayashi K, et, al. Therapeutic effect of arctiin and arctigenin in immunocompetent and immunocompromised mice infected with influenza A virus. Biol Pharm Bull. 2010;33(7):1199-205. [Content Brief]

[4]. Wei L, et, al. Arctigenin Exerts Neuroprotective Effect by Ameliorating Cortical Activities in Experimental Autoimmune Encephalomyelitis In Vivo. Front Immunol. 2021 Jul 19;12:691590. [Content Brief]

[5]. Maxwell T, et, al. Arctigenin inhibits the activation of the mTOR pathway, resulting in autophagic cell death and decreased ER expression in ER-positive human breast cancer cells. Int J Oncol. 2018 Apr;52(4):1339-1349. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6852 mL	13.4261 mL	26.8521 mL	67.1303 mL
	5 mM	0.5370 mL	2.6852 mL	5.3704 mL	13.4261 mL
	10 mM	0.2685 mL	1.3426 mL	2.6852 mL	6.7130 mL
	15 mM	0.1790 mL	0.8951 mL	1.7901 mL	4.4754 mL
	20 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3565 mL
	25 mM	0.1074 mL	0.5370 mL	1.0741 mL	2.6852 mL
	30 mM	0.0895 mL	0.4475 mL	0.8951 mL	2.2377 mL
	40 mM	0.0671 mL	0.3357 mL	0.6713 mL	1.6783 mL
	50 mM	0.0537 mL	0.2685 mL	0.5370 mL	1.3426 mL
	60 mM	0.0448 mL	0.2238 mL	0.4475 mL	1.1188 mL
	80 mM	0.0336 mL	0.1678 mL	0.3357 mL	0.8391 mL
	100 mM	0.0269 mL	0.1343 mL	0.2685 mL	0.6713 mL

Arctigenin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Arctigenin7770-78-7(-)-ArctigeninMMPInfluenza VirusAutophagyApoptosisMatrix metalloproteinasesantitumorantioxidantanti-inflammatoryantiviralinfluenzaAvirusInhibitorinhibitorinhibit

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Arctigenin (Synonyms: (-)-Arctigenin)

Customer Review

Other Forms of Arctigenin:

Arctigenin Related Antibodies

8 Publications Citing Use of MCE Arctigenin

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•Related Screening Libraries:

•Related Small Molecules:

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View All MMP Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Arctigenin Related Antibodies

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Complete Stock Solution Preparation Table

Arctigenin Related Classifications

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