2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Membrane Transporter/Ion Channel Neuronal Signaling Apoptosis
  3. Reactive Oxygen Species Endogenous Metabolite Calcium Channel Sodium Channel Apoptosis NOD-like Receptor (NLR) Bcl-2 Family Caspase
  4. Ascorbyl palmitate

Ascorbyl palmitate  (Synonyms: L-Ascorbic acid 6-hexadecanoate; 6-O-Palmitoyl-L-ascorbic acid)

Cat. No.: HY-B0987 Purity: 99.61%
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Ascorbyl palmitate is an orally active ester formed from ascorbic acid and palmitic acid, used as an antioxidant and food additive. Ascorbyl palmitate in preventing fat and oil oxidation is more efficient than Butylated hydroxyanisole (HY-B1066) and Butylated hydroxytoluene (HY-Y0172). Ascorbyl palmitate mitigates inhibition of collagen synthesis by select calcium and sodium channel blockers. Ascorbyl palmitate induces Apoptosis in human umbilical vein endothelial cells (HUVECs). Ascorbyl palmitate ameliorates inflammatory diseases by inhibition of NLRP3 inflammasome.

For research use only. We do not sell to patients.

Ascorbyl palmitate Chemical Structure

Ascorbyl palmitate Chemical Structure

CAS No. : 137-66-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO USD 55 In-stock
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Based on 3 publication(s) in Google Scholar

Other Forms of Ascorbyl palmitate:

Ascorbyl palmitate Related Antibodies

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

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NLRP3 NLRP1 NOD1 NOD2

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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Description

Ascorbyl palmitate is an orally active ester formed from ascorbic acid and palmitic acid, used as an antioxidant and food additive. Ascorbyl palmitate in preventing fat and oil oxidation is more efficient than Butylated hydroxyanisole (HY-B1066) and Butylated hydroxytoluene (HY-Y0172). Ascorbyl palmitate mitigates inhibition of collagen synthesis by select calcium and sodium channel blockers. Ascorbyl palmitate induces Apoptosis in human umbilical vein endothelial cells (HUVECs). Ascorbyl palmitate ameliorates inflammatory diseases by inhibition of NLRP3 inflammasome[1][2][3][4].

In Vitro

Ascorbyl palmitate (25-400 μM, 24 h and 48 h) induces cell cytotoxicity and inhibits cell growth in HUVECs in a dose- and time-dependent manner[1].
Ascorbyl palmitate (125 μM, 24 h and 48 h) inhibits proliferation of HUVECs and induces cell death by inducing apoptosis, which decreases Bcl-2 gene expression ratio and increases Caspase-3, 9 activities in HUVECs[1].
Ascorbyl palmitate (1.25-20 μM, 72 h) dependent extracellular matrix (ECM) deposition of collagen type l is significantly reduced in aortic smooth muscle cells, which is inhibited by Nifedipine (HY-B0284) (50 µM, 72 h)[2].
Ascorbyl palmitate (0-20 μM, 1 h) has stronger inhibitory activity against NLRP3 inflammasome through its antioxidant ability than ascorbic acid in LPS (HY-D1056)-primed bone marrow-derived macrophages (BMDMs)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ascorbyl palmitate Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Human umbilical vein endothelial cells (HUVECs)
Concentration: 25-400 μM
Incubation Time: 24 h and 48 h
Result: Inhibited cell growth after 24h and 48 h with IC50 values of 440 µM and 125 µM, respectively in HUVECs.

Immunofluorescence[1]

Cell Line: HUVECs
Concentration: 125 μM
Incubation Time: 24 h and 48 h
Result: Resulted that DNA fragmentation which was sign of Apoptosis induction was obvious.

Real Time qPCR[1]

Cell Line: HUVECs
Concentration: 125 μM
Incubation Time: 24 h and 48 h
Result: Decreased Bcl-2 gene expression ratio and increased Caspase-3, 9 activities in HUVECs.

Western Blot Analysis[3]

Cell Line: Mouse BMDMs
Concentration: 5-20 μM
Incubation Time: 1 h
Result: Dose-dependently inhibited the cleavage of caspase-1 in BMDMs.
In Vivo

Ascorbyl palmitate (25 mg/kg, i.p., every 2 days for 10 or 14 days) alleviates LPS (HY-D1056)-induced NLRP3 inflammasome activation, dextran sulfate sodium (DSS) (HY-116282C)-induced colitis and experimental autoimmune encephalomyelitis (EAE) in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice administrated with 2.5 % (w/v) DSS in drinking water for 6 days to induse colitis[3]
Dosage: 25 mg/kg
Administration: i.p., every 2 days for 10 days
Result: Reduced the IL-1β production in serum, colon shortening, loss of goblet cells, destruction of intestinal epithelium structure, infiltration of inflammatory cells in mice with DSS-induced colitis.
Animal Model: Mice immunized with MOG35-55 (HY-P1240) peptide (300 μg, s.c.) on day 0 and Pertussis toxin (HY-112779) (700 ng, i.v.) on days 0 and 2 to induce EAE[3]
Dosage: 25 mg/kg
Administration: i.p., every 2 days for 14 days
Result: Decreased the expression levels of proin-flammatory cytokines, including IL-1β, IL-6, TNF-α in the spinal cord of mice.
Molecular Weight

414.53

Formula

C22H38O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC([C@H](O1)[C@H](COC(CCCCCCCCCCCCCCC)=O)O)=C(O)C1=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (241.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4124 mL 12.0619 mL 24.1237 mL
5 mM 0.4825 mL 2.4124 mL 4.8247 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4124 mL 12.0619 mL 24.1237 mL 60.3093 mL
5 mM 0.4825 mL 2.4124 mL 4.8247 mL 12.0619 mL
10 mM 0.2412 mL 1.2062 mL 2.4124 mL 6.0309 mL
15 mM 0.1608 mL 0.8041 mL 1.6082 mL 4.0206 mL
20 mM 0.1206 mL 0.6031 mL 1.2062 mL 3.0155 mL
25 mM 0.0965 mL 0.4825 mL 0.9649 mL 2.4124 mL
30 mM 0.0804 mL 0.4021 mL 0.8041 mL 2.0103 mL
40 mM 0.0603 mL 0.3015 mL 0.6031 mL 1.5077 mL
50 mM 0.0482 mL 0.2412 mL 0.4825 mL 1.2062 mL
60 mM 0.0402 mL 0.2010 mL 0.4021 mL 1.0052 mL
80 mM 0.0302 mL 0.1508 mL 0.3015 mL 0.7539 mL
100 mM 0.0241 mL 0.1206 mL 0.2412 mL 0.6031 mL
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Ascorbyl palmitate Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ascorbyl137-66-6L-Ascorbic acid 6-hexadecanoate 6-O-Palmitoyl-L-ascorbic acidReactive Oxygen SpeciesEndogenous MetaboliteCalcium ChannelSodium ChannelApoptosisNOD-like Receptor (NLR)Bcl-2 FamilyCaspaseCa2+ channelsCa channelsNa channelsNa+ channelsesterantioxidantfood additiveoxidationcollagen synthesisapoptosisHUVECsinflammatoryInhibitorinhibitorinhibit

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