Buspirone hydrochloride

Name: Buspirone hydrochloride
Brand: MedChemExpress
SKU: HY-B1115
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1115 Purity: 99.99%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Buspirone hydrochloride is a 5-HT1A receptor agonist. Buspirone hydrochloride can be used for anxiety and depression research.

For research use only. We do not sell to patients.

Buspirone hydrochloride Chemical Structure

CAS No. : 33386-08-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Buspirone hydrochloride:

Buspirone-d₈ hydrochloride Get quote
Buspirone-d₈ dihydrochloride Get quote
Buspirone Get quote
Buspirone hydrochloride (Standard) Get quote

1 Publications Citing Use of MCE Buspirone hydrochloride

•Mol Pharmacol. 2023 Nov;104(5):230-238. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Buspirone hydrochloride is a 5-HT1A receptor agonist. Buspirone hydrochloride can be used for anxiety and depression research^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	41 nM Compound: 1	Agonist activity at human 5HT1A receptor expressed in CHO cells by FLIPR Agonist activity at human 5HT1A receptor expressed in CHO cells by FLIPR	[PMID: 19147349]
HEK293	IC₅₀	1.7 μM Compound: buspirone	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	12 μM Compound: buspirone	Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	46 μM Compound: buspirone	Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]

In Vitro

Buspirone hydrochloride (0-400 μg/mL; 6 hours) has cytotoxic effect in Lymphocytes^[1].
Buspirone hydrochloride (0-180 μg/mL; 0-3 hours; Lymphocytes) induces ROS formation, MMP collapse, lipid peroxidation, lysosomal damage and elevation of glutathione disulfide (GSSG)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Lymphocytes
Concentration:	0, 4, 20, 40, 200 and 400 µg/mL
Incubation Time:	6 hours
Result:	Decreased cell viability in a dose-dependent manner.

In Vivo

Buspirone hydrochloride (1-5 mg/kg; i.p. and i.g.; for 5 days; C57BL/6N mice) reduces anxiety/depression behaviors^[2].
? Buspirone hydrochloride (1-5 mg/kg; i.p. and i.g.; for 5 days; C57BL/6N mice) restores IS-shifted β-diversity in the gut microbiota^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6N mice^[2]
Dosage:	1 and 5 mg/kg
Administration:	Oral gavage and intraperitoneal injection; for 5 days
Result:	Reduced TNF-α expression and NF-κB+/Iba1+ cell population in the hippocampus and myeloperoxidase activity and NF-κB+/CD11c+ cell population in the colon.

Animal Model:	Male C57BL/6N mice^[2]
Dosage:	1 and 5 mg/kg
Administration:	Oral gavage and intraperitoneal injection; for 5 days
Result:	Reduced the IS- or EC-induced gut Proteobacteria population.

Clinical Trial

Molecular Weight

421.96

Formula

C₂₁H₃₂ClN₅O₂

CAS No.

33386-08-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(N(CCCCN1CCN(C2=NC=CC=N2)CC1)C(C3)=O)CC43CCCC4.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (236.99 mM)

DMSO : 50 mg/mL (118.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3699 mL	11.8495 mL	23.6989 mL
5 mM	0.4740 mL	2.3699 mL	4.7398 mL
10 mM	0.2370 mL	1.1849 mL	2.3699 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (236.99 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (276 KB) SDS (419 KB)

COA (247 KB) HNMR (274 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Salimi A, et, al. Analysis of Toxicity Effects of Buspirone, Cetirizine and Olanzapine on Human Blood Lymphocytes: in Vitro Model. Curr Clin Pharmacol. 2018;13(2):120-127. [Content Brief]

[2]. Kim JK, et, al. Buspirone alleviates anxiety, depression, and colitis; and modulates gut microbiota in mice. Sci Rep. 2021 Mar 17;11(1):6094. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.3699 mL	11.8495 mL	23.6989 mL	59.2473 mL
	5 mM	0.4740 mL	2.3699 mL	4.7398 mL	11.8495 mL
	10 mM	0.2370 mL	1.1849 mL	2.3699 mL	5.9247 mL
	15 mM	0.1580 mL	0.7900 mL	1.5799 mL	3.9498 mL
	20 mM	0.1185 mL	0.5925 mL	1.1849 mL	2.9624 mL
	25 mM	0.0948 mL	0.4740 mL	0.9480 mL	2.3699 mL
	30 mM	0.0790 mL	0.3950 mL	0.7900 mL	1.9749 mL
	40 mM	0.0592 mL	0.2962 mL	0.5925 mL	1.4812 mL
	50 mM	0.0474 mL	0.2370 mL	0.4740 mL	1.1849 mL
	60 mM	0.0395 mL	0.1975 mL	0.3950 mL	0.9875 mL
	80 mM	0.0296 mL	0.1481 mL	0.2962 mL	0.7406 mL
	100 mM	0.0237 mL	0.1185 mL	0.2370 mL	0.5925 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.