2. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. 5-HT Receptor Dopamine Receptor Reactive Oxygen Species
  4. Buspirone

Buspirone is an orally active 5-HT1A receptor agonist, and a dopamine D2 autoreceptorsant antagonist. Buspirone is an anxiolytic agent, and can be used for the generalized anxiety disorder research.

For research use only. We do not sell to patients.

Buspirone Chemical Structure

Buspirone Chemical Structure

CAS No. : 36505-84-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
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25 mg USD 98 In-stock
50 mg USD 160 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Buspirone:

Buspirone Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Buspirone

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Buspirone is an orally active 5-HT1A receptor agonist, and a dopamine D2 autoreceptorsant antagonist. Buspirone is an anxiolytic agent, and can be used for the generalized anxiety disorder research[1].

IC50 & Target[1]

5-HT1A Receptor

 

Dopamine D2 receptor

 

In Vitro

The anxiolytic and anti-depressant effects of Buspirone are produced by activating 5-HT1A autoreceptors and 5-HT1A heteroreceptors, respectively[1].
Buspirone (0-400 µg/mL; 6 hours) has cytotoxic effect in lymphocytes[2].
Buspirone (0-180 µg/mL; 0-3 hours; lymphocytes) induces ROS formation, mitochondrial membrane potential collapse(MMP), lipid peroxidation, lysosomal damage and elevation of glutathione disulfide (GSSG)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Buspirone Related Antibodies

Cell Viability Assay[2]

Cell Line: Lymphocytes
Concentration: 0, 4, 20, 40, 200 and 400 µg/mL
Incubation Time: 6 hours
Result: Decreased cell viability in a dose-dependent manner.
In Vivo

Buspirone (1-5 mg/kg; i.p. and i.g.; for 5 days; C57BL/6N mice) reduces anxiety/depression behaviors[1].
Buspirone (1-5 mg/kg; i.p. and i.g.; for 5 days; C57BL/6N mice) restores immobilization stress (IS)-shifted β-diversity in the gut microbiota[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6N mice[1]
Dosage: 1 and 5 mg/kg
Administration: Oral gavage and intraperitoneal injection; for 5 days
Result: Reduced TNF-α expression and NF-κB+/Iba1+ cell population in the hippocampus and myeloperoxidase activity and NF-κB+/CD11c+ cell population in the colon.
Animal Model: Male C57BL/6N mice[1]
Dosage: 1 and 5 mg/kg
Administration: Oral gavage and intraperitoneal injection; for 5 days
Result: Reduced the IS- or Escherichia coli K1 (EC)-induced gut Proteobacteria population.
Clinical Trial
Molecular Weight

385.50

Formula

C21H31N5O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N(CCCCN1CCN(C2=NC=CC=N2)CC1)C(C3)=O)CC43CCCC4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (259.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5940 mL 12.9702 mL 25.9403 mL
5 mM 0.5188 mL 2.5940 mL 5.1881 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5940 mL 12.9702 mL 25.9403 mL 64.8508 mL
5 mM 0.5188 mL 2.5940 mL 5.1881 mL 12.9702 mL
10 mM 0.2594 mL 1.2970 mL 2.5940 mL 6.4851 mL
15 mM 0.1729 mL 0.8647 mL 1.7294 mL 4.3234 mL
20 mM 0.1297 mL 0.6485 mL 1.2970 mL 3.2425 mL
25 mM 0.1038 mL 0.5188 mL 1.0376 mL 2.5940 mL
30 mM 0.0865 mL 0.4323 mL 0.8647 mL 2.1617 mL
40 mM 0.0649 mL 0.3243 mL 0.6485 mL 1.6213 mL
50 mM 0.0519 mL 0.2594 mL 0.5188 mL 1.2970 mL
60 mM 0.0432 mL 0.2162 mL 0.4323 mL 1.0808 mL
80 mM 0.0324 mL 0.1621 mL 0.3243 mL 0.8106 mL
100 mM 0.0259 mL 0.1297 mL 0.2594 mL 0.6485 mL
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Buspirone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Buspirone36505-84-75-HT ReceptorDopamine ReceptorReactive Oxygen SpeciesSerotonin Receptor5-hydroxytryptamine Receptor5-HT1A receptoranxietydepressionInhibitorinhibitorinhibit

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