2. Autophagy Neuronal Signaling
  3. Autophagy Beclin1 α-synuclein
  4. Corynoxine B

Corynoxine B is a natural alkaloid and autophagy inducer that can improve Mn induced cellular autophagy dysregulation and enhance clearance of alpha synuclein (alpha syn) in Parkinson's disease mice[1][3].

For research use only. We do not sell to patients.

Corynoxine B Chemical Structure

Corynoxine B Chemical Structure

CAS No. : 17391-18-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Corynoxine B:

Corynoxine B Related Antibodies

Top Publications Citing Use of Products

    Corynoxine B purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2019 Feb;124:336-348.  [Abstract]

    Expression levels of α-SYN and Bcl2 in the whole-cell lysates are determined by western blotting in the control, 200 μM Mn treatment, and Cory B pretreatment groups.

    Corynoxine B purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2019 Feb;124:336-348.  [Abstract]

    The colocalization of HMGB1 (red) and Beclin1 (green), and Bcl2 (red) and Beclin1 (green) in the control, 200 μM Mn treatment, and 100 μM Cory B pretreatment groups.

    Corynoxine B purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2019 Feb;124:336-348.  [Abstract]

    The expression levels of Beclin1, LC3II/I, and p62 are determined by western blotting in the control, 200 μM Mn treatment, and Cory B pretreatment groups.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Corynoxine B is a natural alkaloid and autophagy inducer that can improve Mn induced cellular autophagy dysregulation and enhance clearance of alpha synuclein (alpha syn) in Parkinson's disease mice[1][3].
    Cellular Effect
    Cell Line Type Value Description References
    HL-60 IC50
    > 40 μM
    Compound: 5
    Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
    Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
    		[PMID: 21070010]
    SW480 IC50
    > 40 μM
    Compound: 5
    Cytotoxicity against human SW480 cells after 48 hrs by MTT assay
    Cytotoxicity against human SW480 cells after 48 hrs by MTT assay
    		[PMID: 21070010]
    In Vitro

    Corynoxine B (25-100 μM, 2 h) can improve Mn induced autophagy dysregulation and neurotoxicity in SH-SY5Y human neuroblastoma cells[1].
    Corynoxine B can enhance the expression of BECN1 protein and restore autophagy inhibition caused by overexpression of SNCA/α-synuclein (α-syn) in PC12 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Corynoxine B Related Antibodies

    Cell Viability Assay[1].

    Cell Line: SH-SY5Y cell
    Concentration: 25-100 μM
    Incubation Time: 2 h
    Result: Improved cell vitality

    Western Blot Analysis[2].

    Cell Line: Inducible PC12 cell lines (iPC12) overexpressing SNCA (WT and A53T)
    Concentration:
    Incubation Time: 24 h
    Result: Increased LC3-II and BECN1 protein levels
    In Vivo

    Corynoxine B (5-20 mg/kg, i.p.) enhances autophagy of alpha synuclein (alpha syn) clearance in Parkinson's disease mice by targeting HMGB1/2[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: A53T α-syn transgenic mouse model[3].
    Dosage: 5-20 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Promoted α-syn clearance
    Molecular Weight

    384.47

    Formula

    C22H28N2O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NC1=C2C=CC=C1)[C@]32[C@@](C[C@H](/C(C(OC)=O)=C\OC)[C@H](CC)C4)([H])N4CC3
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 83.33 mg/mL (216.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6010 mL 13.0049 mL 26.0098 mL
    5 mM 0.5202 mL 2.6010 mL 5.2020 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.76%

    SDS (479 KB)

    COA (251 KB) LCMS (270 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6010 mL 13.0049 mL 26.0098 mL 65.0246 mL
    5 mM 0.5202 mL 2.6010 mL 5.2020 mL 13.0049 mL
    10 mM 0.2601 mL 1.3005 mL 2.6010 mL 6.5025 mL
    15 mM 0.1734 mL 0.8670 mL 1.7340 mL 4.3350 mL
    20 mM 0.1300 mL 0.6502 mL 1.3005 mL 3.2512 mL
    25 mM 0.1040 mL 0.5202 mL 1.0404 mL 2.6010 mL
    30 mM 0.0867 mL 0.4335 mL 0.8670 mL 2.1675 mL
    40 mM 0.0650 mL 0.3251 mL 0.6502 mL 1.6256 mL
    50 mM 0.0520 mL 0.2601 mL 0.5202 mL 1.3005 mL
    60 mM 0.0433 mL 0.2167 mL 0.4335 mL 1.0837 mL
    80 mM 0.0325 mL 0.1626 mL 0.3251 mL 0.8128 mL
    100 mM 0.0260 mL 0.1300 mL 0.2601 mL 0.6502 mL
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    Corynoxine B Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Corynoxine B17391-18-3AutophagyBeclin1α-synucleinAtg6α-SynautophagyalkaloidParkinsonInhibitorinhibitorinhibit

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