Desmosterol

Name: Desmosterol
Brand: MedChemExpress
SKU: HY-113224
Rating: 5.0 (5 reviews)

Cat. No.: HY-113224 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Desmosterol is a cholesterol-like molecule. In the Bloch pathway of cholesterol biosynthesis, Desmosterol is a direct precursor of cholesterol. As an endogenous metabolite, Desmosterol is used to study cholesterol metabolism . Desmosterol is an LXR activator and SREBP inhibitor, which can suppress macrophage inflammasome activation and prevent vascular inflammation and atherosclerosis. A reduction in Desmosterol promotes the production of mitochondrial reactive oxygen species (ROS) in macrophages and pyrin domain-dependent inflammasome activation of NLRP3. Desmosterol holds potential for research in inflammation, metabolism, and cardiovascular diseases .

For research use only. We do not sell to patients.

Desmosterol Chemical Structure

CAS No. : 313-04-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMF ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMF	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Desmosterol:

Desmosterol-d₆ Get quote
Desmosterol (Standard) Get quote

5 Publications Citing Use of MCE Desmosterol

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NLRP3 NLRP1 NOD1 NOD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Desmosterol is a cholesterol-like molecule. In the Bloch pathway of cholesterol biosynthesis, Desmosterol is a direct precursor of cholesterol. As an endogenous metabolite, Desmosterol is used to study cholesterol metabolism ^[1]. Desmosterol is an LXR activator and SREBP inhibitor, which can suppress macrophage inflammasome activation and prevent vascular inflammation and atherosclerosis. A reduction in Desmosterol promotes the production of mitochondrial reactive oxygen species (ROS) in macrophages and pyrin domain-dependent inflammasome activation of NLRP3. Desmosterol holds potential for research in inflammation, metabolism, and cardiovascular diseases ^[1]^[2]^[3].

IC₅₀ & Target^[1]

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	> 50 μg/mL Compound: 1, desmosterol	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 8984148]
HT-29	ED₅₀	> 50 μg/mL Compound: 1, desmosterol	Cytotoxicity against human HT29 cells by MTT assay Cytotoxicity against human HT29 cells by MTT assay	[PMID: 8984148]
KB	ED₅₀	> 50 μg/mL Compound: 1, desmosterol	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 8984148]
P388	ED₅₀	33.53 μg/mL Compound: 1, desmosterol	Cytotoxicity against mouse P388 cells by MTT assay Cytotoxicity against mouse P388 cells by MTT assay	[PMID: 8984148]

In Vitro

Desmosterol depletion (by activating the DHCR24 gene in Dhcr24TG mice to lower Desmosterol levels in macrophages for 4 weeks) inhibits LXR activation, upregulates immune response genes, suppresses cholesterol efflux, thereby leading to lipid accumulation in macrophages and foam cell formation, which is closely associated with the development of atherosclerosis disease ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Mouse peritoneal macrophages (TGEM) and human hepatocyte cell line (HepG2)
Concentration:	10 μM
Incubation Time:	48 hours
Result:	Activated Abca1 protein expression in TGEMs but had no significant effect in HepG2 cells.

Real Time qPCR^[3]

Cell Line:	Mouse peritoneal macrophages (TGEM)
Concentration:	10 μM
Incubation Time:	48 h
Result:	Suppressed the expression of the SREBP target gene Dhcr24.

In Vivo

Desmosterol depletion (by intraperitoneal injection of Adenovirus (AAV-Cre) to activate the DHCR24 gene in Dhcr24TG mice, thereby reducing Desmosterol levels in macrophages) would suppress LXR activation, upregulate immune response genes, inhibit cholesterol efflux, leading to lipid accumulation in macrophages and foam cell formation, which is closely associated with the development of atherosclerosis disease ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Dhcr24TG and WT mice Note: 1. Dhcr24TG mice: Crossing Dhcr24fl/fl mice with LysM-Cre mice to obtain conditional Dhcr24TG mice, ensuring that DHCR24 was overexpressed only in macrophages 2. DHCR24 catalyzed the final step in the conversion of Desmosterol to cholesterol. Activating the DHCR24 gene decreased the level of Desmosterol.
Dosage:	This experiment did not involve drug administration but altered Desmosterol levels through genetic engineering: 1. Adenovirus (AAV-Cre) was injected (i.p.) to activate DHCR24 in Dhcr24fl/fl mice. 2. After genetic modification, macrophages were isolated from blood and analyzed via Western Blot after 2 weeks of AAV-Cre injection.
Administration:	Samples of macrophages were collected starting from 2 weeks after the virus injection, with the total experimental period lasting 4 weeks.
Result:	Significantly reduced the LXR signaling in Dhcr24TG mice, and downregulated LXR-dependent genes like ABCA1 and ABCG1 (Desmosterol depletion). Increased expression of inflammatory genes like Cxcl9, Cxcl10, Il6 in Dhcr24TG macrophages.

Molecular Weight

384.64

Formula

C27H44O

CAS No.

313-04-2

Appearance

Solid

Color

White to off-white

SMILES

C/C(C)=C\CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Structure Classification

Steroids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMF : 50 mg/mL (129.99 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5998 mL	12.9992 mL	25.9983 mL
5 mM	0.5200 mL	2.5998 mL	5.1997 mL
10 mM	0.2600 mL	1.2999 mL	2.5998 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (248 KB) HNMR (266 KB)

Handling Instructions (2659 KB)

References

[1]. Clark RM, et al. Desmosterol in human milk. Lipids. 1983;18(3):264-266. [Content Brief]

[2]. Zhang X, et al. Desmosterol suppresses macrophage inflammasome activation and protects against vascular inflammation and atherosclerosis. Proc Natl Acad Sci U S A. 2021 Nov 23;118(47):e2107682118. [Content Brief]

[3]. Muse ED, et al. Cell-specific discrimination of desmosterol and desmosterol mimetics confers selective regulation of LXR and SREBP in macrophages. Proc Natl Acad Sci U S A. 2018 May 15;115(20):E4680-E4689. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF	1 mM	2.5998 mL	12.9992 mL	25.9983 mL	64.9958 mL
	5 mM	0.5200 mL	2.5998 mL	5.1997 mL	12.9992 mL
	10 mM	0.2600 mL	1.2999 mL	2.5998 mL	6.4996 mL
	15 mM	0.1733 mL	0.8666 mL	1.7332 mL	4.3331 mL
	20 mM	0.1300 mL	0.6500 mL	1.2999 mL	3.2498 mL
	25 mM	0.1040 mL	0.5200 mL	1.0399 mL	2.5998 mL
	30 mM	0.0867 mL	0.4333 mL	0.8666 mL	2.1665 mL
	40 mM	0.0650 mL	0.3250 mL	0.6500 mL	1.6249 mL
	50 mM	0.0520 mL	0.2600 mL	0.5200 mL	1.2999 mL
	60 mM	0.0433 mL	0.2167 mL	0.4333 mL	1.0833 mL
	80 mM	0.0325 mL	0.1625 mL	0.3250 mL	0.8124 mL
	100 mM	0.0260 mL	0.1300 mL	0.2600 mL	0.6500 mL

Desmosterol Related Classifications

Metabolic Disease Inflammation/Immunology Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Desmosterol313-04-2Endogenous MetaboliteLXRFatty Acid Synthase (FASN)Interleukin RelatedReactive Oxygen SpeciesNOD-like Receptor (NLR)Liver X receptorILmoleculesimilarendogenousmetabolitecholesterolBlochInhibitorinhibitorinhibit

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Desmosterol

Customer Review

Other Forms of Desmosterol:

Desmosterol Related Antibodies

5 Publications Citing Use of MCE Desmosterol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Interleukin Related Isoform Specific Products:

View All NOD-like Receptor (NLR) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Desmosterol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Desmosterol Related Classifications

Keywords:

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Customer Review

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