2. Metabolic Enzyme/Protease Anti-infection
  3. Endogenous Metabolite SARS-CoV
  4. Pantethine

Pantethine  (Synonyms: D-Pantethine; LBF disulfide)

Cat. No.: HY-B1028 Purity: 99.91%
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Pantethine is an orally active lipid-lowering agent. Pantethine has anti-tumor, anti-inflammatory and anti-SARS-COV virus activities. Pantethine is also a neuroprotective agent. Pantethine can be used in the study of Alzheimer's disease, Parkinson's disease, major depression, systemic sclerosis and pantothenate kinase-related neurodegeneration.

For research use only. We do not sell to patients.

Pantethine Chemical Structure

Pantethine Chemical Structure

CAS No. : 16816-67-4

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid or liquid
100 mg USD 60 In-stock
500 mg USD 84 In-stock
1 g USD 117 In-stock
5 g USD 355 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Pantethine:

Pantethine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Pantethine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pantethine is an orally active lipid-lowering agent. Pantethine has anti-tumor, anti-inflammatory and anti-SARS-COV virus activities. Pantethine is also a neuroprotective agent. Pantethine can be used in the study of Alzheimer's disease, Parkinson's disease, major depression, systemic sclerosis and pantothenate kinase-related neurodegeneration[1][2][3][4][5][6].

In Vitro

Pantethine (10-1000 μM) inhibits cholesterol production in mouse liver slices[1].
Pantethine (250-1000 μM; 24 h and 72 h) reduces cholesterol levels in Vero E6 cells (35% at 24 h and 80% at 72 h)[2].
Pantethine(100-1000 μM; After pretreatment for 1 h, the virus is added and incubated together for 2 h until the end of the experiment) reduces the infection of SARS-CoV-2 to Vero E6 and Calu-3a cells in a dose-dependent manner, which has antiviral effect[2].
Pantethine (50-100 μM; 18 h) inhibits the release of microparticles (MPs) in a dose-dependent manner in endothelial HPMECs and HUVECs. (50-100 μM; 24 h) eliminates MP-induced oxidative and nitrosation stress in endothelial cells (HPMECs and HUVECs) and fibroblasts (NIH3T3)[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pantethine Related Antibodies

Western Blot Analysis [2]

Cell Line: Calu-3a, Vero E6
Concentration: 50 μM, 100 μM, 250 μM, 500 μM, 1000 μM
Incubation Time: Cell were pretreated with Pantethine for 1 h prior to virus infection, followed by incubation with the virus for 2 h in the presence of Pantethine until the end of the experiment.
Result: Decreased the expression of viral spike (S) and nucleocapsid (N) proteins.

RT-PCR [2]

Cell Line: Calu-3a, Vero E6
Concentration: 50 μM, 100 μM, 250 μM, 500 μM, 1000 μM
Incubation Time: Cell were pretreated with Pantethine for 1 h prior to virus infection, followed by incubation with the virus for 2 h in the presence of Pantethine until the end of the experiment.
Result: Significantly reduced the copy number of viral nucleapsid (N) and non-structural protein 6 (NSP6) genes in the cell supernatant.
Decreased the mRNA expression levels of HECT E3 ligase and TMPRSS2 in virus-induced infected cells.
Significantly decreased the mRNA expression of MAVS, IRF3, IFNβ, STING, TNF-α and IL6 in Calu-3a cells, and decreased the inflammation caused by SARS-CoV-2 virus infection.
In Vivo

Pantethine (1.2 mmol/kg; Intragastrical administration (i.g.); Single dose) can reduce blood lipid in rats[1].
Pantethine (750 mg/kg; Intraperitoneal injection (i.p.); Once daily for 4 weeks) shows antitumor activity in mice with in-situ ovarian cancer [3].
Pantethine (150 mg/kg; p.o.; Once daily for 6 weeks) reduces skin and lung fibrosis associated with markers of oxidative and endothelial stress in mice with systemic sclerosis (SSc)[5].
Pantethine (15 mg/kg; Supplemented in daily drinking water, for 2 months) is able to effectively restore the disease status induced by the ketogenic diet in mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague–Dawley rats model[1]
Dosage: 1.2 mmol/kg
Administration: Intragastrical administration (i.g.); Single dose
Result: Reduced plasma free fatty acid (FFA), cholesterol, and triglyceride levels at 3, 5, 7, and 24 h, while significantly increased glycerol levels at 7 h.
Animal Model: Orthotopic female rats model of ovarian cancer[3]
Dosage: 750 mg/kg
Administration: Intraperitoneal injection (i.p.); Once daily for 4 weeks
Result: Reduced tumor growth, metastasis and ascites, and had no toxicity to liver and kidney.
Animal Model: Systemic sclerosis (SSc) BALB/c mice model[5]
Dosage: 15 mg/kg
Administration: Oral gavage (p.o.); Once daily for 6 weeks. After HOCL treatment ( 200 μM; Intradermal injection; Once daiy for 6 weeks).
Result: Decreased the number of MPs in mice endothelial cells.
Decreased the concentrations of soluble E-selectin and sVCAM-1 in serum.
Decreased serum concentration of AOPPs (33%) and nitrate (60%).
Animal Model: Ketogenic diet mouse model[6]
Dosage: 150 mg/kg
Administration: Supplemented in daily drinking water, for 2 months. Follow a casual ketogenic diet during this period.
Result: Alleviated motor dysfunction, neurodegeneration and changes in mitochondria of the central and peripheral nervous systems in mice.
Clinical Trial
Molecular Weight

554.72

Formula

C22H42N4O8S2
CAS No.
Appearance

Solid-Liquid Mixture

Color

Off-white to light brown

SMILES

O=C(NCCSSCCNC(CCNC([C@H](O)C(C)(C)CO)=O)=O)CCNC([C@H](O)C(C)(C)CO)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (180.27 mM)

DMSO : ≥ 100 mg/mL (180.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (180.27 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8027 mL 9.0136 mL 18.0271 mL
5 mM 0.3605 mL 1.8027 mL 3.6054 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (180.27 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.91%

COA (241 KB) HNMR (138 KB) RP-HPLC (262 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO / Ethanol 1 mM 1.8027 mL 9.0136 mL 18.0271 mL 45.0678 mL
5 mM 0.3605 mL 1.8027 mL 3.6054 mL 9.0136 mL
10 mM 0.1803 mL 0.9014 mL 1.8027 mL 4.5068 mL
15 mM 0.1202 mL 0.6009 mL 1.2018 mL 3.0045 mL
20 mM 0.0901 mL 0.4507 mL 0.9014 mL 2.2534 mL
25 mM 0.0721 mL 0.3605 mL 0.7211 mL 1.8027 mL
30 mM 0.0601 mL 0.3005 mL 0.6009 mL 1.5023 mL
40 mM 0.0451 mL 0.2253 mL 0.4507 mL 1.1267 mL
50 mM 0.0361 mL 0.1803 mL 0.3605 mL 0.9014 mL
60 mM 0.0300 mL 0.1502 mL 0.3005 mL 0.7511 mL
80 mM 0.0225 mL 0.1127 mL 0.2253 mL 0.5633 mL
100 mM 0.0180 mL 0.0901 mL 0.1803 mL 0.4507 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pantethine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pantethine16816-67-4D-Pantethine LBF disulfideEndogenous MetaboliteSARS-CoVSARS coronavirusOvarian cancerVero E6Calu-3ablood lipidsNeuroprotectionAlzheimer's diseaseParkinson's diseaseMajor depressionSystemic sclerosis (SSc)Pantothenate kinase-associated neurodegenerationHPMECsHUVECsNIH3T3Inhibitorinhibitorinhibit

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