Stachydrine hydrochloride

Name: Stachydrine hydrochloride
Brand: MedChemExpress
SKU: HY-N0738
Rating: 5.0 (4 reviews)

Cat. No.: HY-N0738 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Stachydrine hydrochloride is the major active constituent of Leonurus artemisia, which is a potential therapy for cardiovascular diseases. Stachydrine can inhibit the NF-κB signal pathway. Anti-hypertrophic activities.

For research use only. We do not sell to patients.

Stachydrine hydrochloride Chemical Structure

CAS No. : 4136-37-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Stachydrine hydrochloride:

Stachydrine In-stock
Stachydrine hydrochloride (Standard) Get quote

4 Publications Citing Use of MCE Stachydrine hydrochloride

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

View All Isoforms

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Stachydrine hydrochloride is the major active constituent of Leonurus artemisia, which is a potential therapy for cardiovascular diseases^[2]. Stachydrine can inhibit the NF-κB signal pathway. Anti-hypertrophic activities^[1].

IC₅₀ & Target^[1]

p65	Human Endogenous Metabolite

In Vitro

Intervention of Stachydrine significantly suppresses the level of p-IκB (ser32) protein in the cytosol and NF-κB (p65) protein in the nucleus (P<0.05)^[1].
Treatment with Stachydrine hydrochloride (50 μM, 200 μM, 500 μM and 1000 μM) noticeably inhibited MCF-7 and T47D cell proliferation in dose- and time-dependent manner^[2].
High concentrations (500 μM and 1000 μM) of Stachydrine significantly increased the frequency of both studied cell lines at the G1 phase of cell cycle, suggesting that Stachydrine hydrochloride could cause cell cycle arrest^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

179.64

Formula

C₇H₁₄ClNO₂

CAS No.

4136-37-2

Appearance

Solid

Color

White to off-white

SMILES

OC([C@H](CCC1)[N+]1(C)C)=O.[Cl-]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (556.67 mM; Need ultrasonic)

DMSO : 100 mg/mL (556.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.5667 mL	27.8334 mL	55.6669 mL
5 mM	1.1133 mL	5.5667 mL	11.1334 mL
10 mM	0.5567 mL	2.7833 mL	5.5667 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (16.70 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (16.70 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (16.70 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (556.67 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (255 KB) HNMR (92 KB)

Handling Instructions (2659 KB)

References

[1]. Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. [Content Brief]

[2]. Wang M, et al. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways. Am J Transl Res. 2017 Apr 15;9(4):1834-1844. [Content Brief]

Cell Assay

Cell Proliferation Assay^[2]
Cell Cycle Analysis^[2]
MCF-7 and T47D cells
50 µM, 200 µM, 500 µM and 1000 µM
24 and 48 hours
CCK-8 cell counting assay is used to detect proliferation of MCF-7 and T47D cells when treated with Stachydrine hydrochloride at four different concentrations (50 µM, 200 µM, 500 µM and 1000 µM) for 24 and 48 hours. Cell cycle analysis of MCF-7 and T47D cells is determined by flow cytometry. Experiments are performed in triplicates and representative images are presented.
Note: Inhibited proliferation and induced G1 phase arrest in MCF-7 and T47D cells.
[1] Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. https://www.ncbi.nlm.nih.gov/pubmed/23236831 [2] Am J Transl Res. 2017 Apr 15;9(4):1834-1844. eCollection 2017. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways. Wang M1, Shu ZJ1, Wang Y1, Peng W1 https://www.ncbi.nlm.nih.gov/pubmed/28469788

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. [Content Brief]

[2]. Wang M, et al. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways. Am J Transl Res. 2017 Apr 15;9(4):1834-1844. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.5667 mL	27.8334 mL	55.6669 mL	139.1672 mL
	5 mM	1.1133 mL	5.5667 mL	11.1334 mL	27.8334 mL
	10 mM	0.5567 mL	2.7833 mL	5.5667 mL	13.9167 mL
	15 mM	0.3711 mL	1.8556 mL	3.7111 mL	9.2778 mL
	20 mM	0.2783 mL	1.3917 mL	2.7833 mL	6.9584 mL
	25 mM	0.2227 mL	1.1133 mL	2.2267 mL	5.5667 mL
	30 mM	0.1856 mL	0.9278 mL	1.8556 mL	4.6389 mL
	40 mM	0.1392 mL	0.6958 mL	1.3917 mL	3.4792 mL
	50 mM	0.1113 mL	0.5567 mL	1.1133 mL	2.7833 mL
	60 mM	0.0928 mL	0.4639 mL	0.9278 mL	2.3195 mL
	80 mM	0.0696 mL	0.3479 mL	0.6958 mL	1.7396 mL
	100 mM	0.0557 mL	0.2783 mL	0.5567 mL	1.3917 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.