Subasumstat (Synonyms: TAK-981)

Name: Subasumstat
Brand: MedChemExpress
SKU: HY-111789
Rating: 5.0 (13 reviews)

Cat. No.: HY-111789 Purity: 98.56%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Subasumstat (TAK-981) is a first in class and selective inhibitor of the SUMOylation enzymatic cascade, with potential immune-activating and antineoplastic activities.

For research use only. We do not sell to patients.

Subasumstat Chemical Structure

CAS No. : 1858276-04-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 254	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 254	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 593	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Subasumstat:

(S)-Subasumstat Get quote

11 Publications Citing Use of MCE Subasumstat

RT-PCR

Proliferation Assay

: Subasumstat purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Feb 21;14(2):147. [Abstract]; Subasumstat (TAK-981; 25 mg/kg; every 3 days for 21 days) suppresses the growth of glioblastoma in mice.

: Subasumstat purchased from MedChemExpress. Usage Cited in: Nat Microbiol. 2022 Nov 14. [Abstract]; Subasumstat (TAK-981) boosts NLuc expression from unintegrated HIV-1 DNA, reaching a plateau at ~75 nM. Subasumstat is added to CEM-SS Cas9 and ΔSMC5 cells infected with NL-NLuc at 0, 5, 15, 30, 75, 150, 500 and 1,000 nM concentrations, and the cells are collected for an NLuc assay after 2 dpi.

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Biological Activity
Purity & Documentation
References
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Description

Subasumstat (TAK-981) is a first in class and selective inhibitor of the SUMOylation enzymatic cascade, with potential immune-activating and antineoplastic activities^[1]^[2].

IC₅₀ & Target

SUMOylation^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	EC₅₀	0.063 μM Compound: TAK-981	Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 33631934]
OCI-Ly10	EC₅₀	0.004 μM Compound: TAK-981	Cytotoxicity against human OCI-LY10 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human OCI-LY10 cells assessed as reduction in cell viability after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 33631934]

In Vitro

Subasumstat (TAK-981) is able to increase the production of type 1 IFN, thereby increasing type 1 IFN-mediated signaling, activating innate effector cells and enhancing the antitumor innate immune responses^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

A single sub-cutaneous injection of Subasumstat (TAK-981) in naive Balb/c mice at the brachial lymph nodes induces activation of DCs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

578.10

Formula

C₂₅H₂₈ClN₅O₅S₂

CAS No.

1858276-04-6

Appearance

Solid

Color

White to light yellow

SMILES

O=S(OC[C@@H]1[C@@H](O)C[C@H](NC2=NC=NC=C2C(C3=CC([C@@H]4NCCC5=C4C=C(Cl)C=C5)=C(C)S3)=O)C1)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (172.98 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7298 mL	8.6490 mL	17.2980 mL
5 mM	0.3460 mL	1.7298 mL	3.4596 mL
10 mM	0.1730 mL	0.8649 mL	1.7298 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.32 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.56%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (256 KB) HNMR (338 KB) LCMS (150 KB)

Handling Instructions (2659 KB)

References

[1]. Sumoylation inhibitor TAK-981.

[2]. TAK-981.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7298 mL	8.6490 mL	17.2980 mL	43.2451 mL
	5 mM	0.3460 mL	1.7298 mL	3.4596 mL	8.6490 mL
	10 mM	0.1730 mL	0.8649 mL	1.7298 mL	4.3245 mL
	15 mM	0.1153 mL	0.5766 mL	1.1532 mL	2.8830 mL
	20 mM	0.0865 mL	0.4325 mL	0.8649 mL	2.1623 mL
	25 mM	0.0692 mL	0.3460 mL	0.6919 mL	1.7298 mL
	30 mM	0.0577 mL	0.2883 mL	0.5766 mL	1.4415 mL
	40 mM	0.0432 mL	0.2162 mL	0.4325 mL	1.0811 mL
	50 mM	0.0346 mL	0.1730 mL	0.3460 mL	0.8649 mL
	60 mM	0.0288 mL	0.1442 mL	0.2883 mL	0.7208 mL
	80 mM	0.0216 mL	0.1081 mL	0.2162 mL	0.5406 mL
	100 mM	0.0173 mL	0.0865 mL	0.1730 mL	0.4325 mL

Subasumstat Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Subasumstat1858276-04-6TAK-981TAK981TAK 981E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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Subasumstat (Synonyms: TAK-981)

Customer Review

Other Forms of Subasumstat:

Subasumstat Related Antibodies

11 Publications Citing Use of MCE Subasumstat

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References

Customer Review

Subasumstat Related Antibodies

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Complete Stock Solution Preparation Table

Subasumstat Related Classifications

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