2'-Deoxyadenosine

Name: 2'-Deoxyadenosine
Brand: MedChemExpress
SKU: HY-W040329
Rating: 4.5 (1 reviews)

Cat. No.: HY-W040329 Purity: 99.99%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

2'-Deoxyadenosine is a purine nucleoside composed of Adenine (HY-B0152) and 2'-deoxyribose. 2'-Deoxyadenosine activates Caspase-3 and promotes Apoptosis. 2'-Deoxyadenosine inhibits the activity of S-adenosyl-L-homocysteine hydrolase. 2'-Deoxyadenosine inhibits the growth of various cells. 2'-Deoxyadenosine has an anticancer effect on colon cancer.

For research use only. We do not sell to patients.

2'-Deoxyadenosine Chemical Structure

CAS No. : 958-09-8

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 2'-Deoxyadenosine:

2'-Deoxyadenosine monohydrate In-stock
2'-Deoxyadenosine-¹³C₁₀,¹⁵N₅ Get quote
2'-Deoxyadenosine-¹³C₁₀ Get quote
2'-Deoxyadenosine-d₁₃ Get quote
2'-Deoxyadenosine-¹⁵N₅,d₁₃ Get quote
2'-Deoxyadenosine-¹⁵⁵ Get quote
2'-Deoxyadenosine-¹³C₅ monohydrate Get quote
2'-Deoxyadenosine (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
C6	IC₅₀	> 250 μM Compound: 2'-Deoxyadenosine	Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against rat C6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
DU-145	IC₅₀	> 250 μM Compound: 2'-Deoxyadenosine	Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
HaCaT	IC₅₀	> 250 μM Compound: 2'-Deoxyadenosine	Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human HaCaT cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
L6	IC₅₀	> 250 μM Compound: 2'-Deoxyadenosine	Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against rat L6 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H
MCF7	IC₅₀	> 250 μM Compound: 2'-Deoxyadenosine	Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	10.1039/C3MD00159H

In Vitro

2'-Deoxyadenosine (0.01-0.5 mM; 24-72 h) inhibits the growth of LoVo human colon carcinoma cells in a concentration- and time-dependent manner and promotes apoptosis, as indicated by characteristic apoptotic morphology^[1].
2'-Deoxyadenosine inhibits S-adenosyl-L-homocysteine hydrolase activity in crude extracts of L1210 leukemia cells in a time-dependent manner when assays are conducted in the presence of Deoxycoformycin (HY-A0006)^[2].
2'-Deoxyadenosine (0.1-2 mM; up to 3 h) inhibits pig keratinocyte outgrowth, [³H] thymidine uptake, and mitosis^[3].
2'-Deoxyadenosine (0.1 mM; 4 days) in combination with 1 μM dCF inhibits the growth of LoVo cells in a cell-density-dependent manner^[4].
2'-Deoxyadenosine (100 μM; 3 days) in the presence of 3 μM Deoxycoformycin (HY-A0006) is toxic to rat chromaffin cells, causing apoptosis-like cell death, as shown by morphological changes, DNA fragmentation, and increased dATP levels^[5].
2'-Deoxyadenosine (0.1 mM) in the presence of 0.01 mM dCF in vitro leads to a significant increase in dATP levels in mouse embryos^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	LoVo human colon carcinoma cell line
Concentration:	0.01 mM, 0.02 mM, 0.05 mM, 0.1 mM, 0.2 mM, 0.5 mM
Incubation Time:	24 h, 48 h, 72 h
Result:	Increased the percentage of apoptotic cells, reaching a maximum of approximately 25% after 72 h (0.5 mM). Processed procaspase-3 and released cytochrome c.

Molecular Weight

251.25

Formula

C₁₀H₁₃N₅O₃

CAS No.

958-09-8

Appearance

Solid

Color

White to off-white

SMILES

NC1=NC=NC2=C1N=CN2[C@H]3C[C@H](O)[C@@H](CO)O3

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (398.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 16.67 mg/mL (66.35 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9802 mL	19.9008 mL	39.8016 mL
5 mM	0.7960 mL	3.9802 mL	7.9603 mL
10 mM	0.3980 mL	1.9901 mL	3.9802 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (280 KB) SDS (479 KB)

COA (254 KB) HNMR (253 KB) RP-HPLC (197 KB)

Handling Instructions (2659 KB)

References

[1]. Giannecchini M, et al. 2'-Deoxyadenosine causes apoptotic cell death in a human colon carcinoma cell line. J Biochem Mol Toxicol. 2003;17(6):329-37. [Content Brief]

[2]. Cass CE, et al. Effects of 2'-deoxyadenosine, 9-beta-D-arabinofuranosyladenine, and related compounds on S-adenosyl-L-homocysteine hydrolase activity in synchronous and asynchronous cultured cells. Cancer Res. 1982 Dec;42(12):4991-8. [Content Brief]

[3]. Iizuka H, et al. Effects of adenosine and 2'-deoxyadenosine on epidermal keratinocyte proliferation: its relation to cyclic AMP formation. J Invest Dermatol. 1984 Jun;82(6):608-12. [Content Brief]

[4]. Bemi V, et al. Deoxyadenosine metabolism in a human colon-carcinoma cell line (LoVo) in relation to its cytotoxic effect in combination with deoxycoformycin. Int J Cancer. 1998 Mar 2;75(5):713-20. [Content Brief]

[5]. Wakade AR, et al. 2'-deoxyadenosine induces apoptosis in rat chromaffin cells. J Neurochem. 1996 Dec;67(6):2273-81. [Content Brief]

[6]. Gao X, et al. Activation of apoptosis in early mouse embryos by 2'-deoxyadenosine exposure. Teratology. 1994 Jan;49(1):1-12. [Content Brief]

[7]. Kuttesch JF Jr, et al. Renal handling of 2'-deoxyadenosine and adenosine in humans and mice. Cancer Chemother Pharmacol. 1982;8(2):221-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.9802 mL	19.9008 mL	39.8016 mL	99.5041 mL
	5 mM	0.7960 mL	3.9802 mL	7.9603 mL	19.9008 mL
	10 mM	0.3980 mL	1.9901 mL	3.9802 mL	9.9504 mL
	15 mM	0.2653 mL	1.3267 mL	2.6534 mL	6.6336 mL
	20 mM	0.1990 mL	0.9950 mL	1.9901 mL	4.9752 mL
	25 mM	0.1592 mL	0.7960 mL	1.5921 mL	3.9802 mL
	30 mM	0.1327 mL	0.6634 mL	1.3267 mL	3.3168 mL
	40 mM	0.0995 mL	0.4975 mL	0.9950 mL	2.4876 mL
	50 mM	0.0796 mL	0.3980 mL	0.7960 mL	1.9901 mL
	60 mM	0.0663 mL	0.3317 mL	0.6634 mL	1.6584 mL
DMSO	80 mM	0.0498 mL	0.2488 mL	0.4975 mL	1.2438 mL
DMSO	100 mM	0.0398 mL	0.1990 mL	0.3980 mL	0.9950 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.