Amitriptyline

Cat. No.: HY-B0527 Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

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Amitriptyline is a common oral anticholinergic. Amitriptyline has anticholinergic properties. Amitriptyline can be used to quantify anticholinergic adverse reactions in patients taking amitriptyline and placebo .

For research use only. We do not sell to patients.

Amitriptyline Chemical Structure

CAS No. : 50-48-6

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Liquid
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 4 publication(s) in Google Scholar

Other Forms of Amitriptyline:

Amitriptyline hydrochloride In-stock
Amitriptyline hydrochloride (Standard) Get quote

4 Publications Citing Use of MCE Amitriptyline

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	3 μM Compound: Amitryptline	Inhibition of human ERG expressed in CHO cells at -80 mV holding potential by whole-cell patch clamp assay Inhibition of human ERG expressed in CHO cells at -80 mV holding potential by whole-cell patch clamp assay	[PMID: 30653317]
CHO-K1	IC₅₀	1.6 μM Compound: amitriptyline	Inhibition of human NaV1.5 alpha subunit expressed in CHOK1 cells at -90 mV holding potential by patch clamp electrophysiological assay Inhibition of human NaV1.5 alpha subunit expressed in CHOK1 cells at -90 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
CHO-K1	IC₅₀	3.1 μM Compound: amitriptyline	Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	1 μM Compound: amitriptyline	Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	12 μM Compound: amitriptyline	Inhibition of human NaV1.7 F1737A mutant expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay Inhibition of human NaV1.7 F1737A mutant expressed in HEK293 cells at -65 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	16.9 μM Compound: amitriptyline	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	[PMID: 18788725]
HEK293	IC₅₀	7 μM Compound: amitriptyline	Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -90 mV holding potential by patch clamp electrophysiological assay Inhibition of recombinant human NaV1.7 alpha subunit expressed in HEK293 cells at -90 mV holding potential by patch clamp electrophysiological assay	[PMID: 22770500]
HEK293	IC₅₀	9.6 μM Compound: Amitriptyline	Inhibition of [3H]-dofetilide binding to human ERG expressed in HEK293 cell membranes measured after 1 hr by liquid scintillation counting method Inhibition of [3H]-dofetilide binding to human ERG expressed in HEK293 cell membranes measured after 1 hr by liquid scintillation counting method	[PMID: 30597328]
HepG2	IC₅₀	29.6 μM Compound: Amitriptyline	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by measuring ATP content incubated for 24 hrs by luminescent analysis Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by measuring ATP content incubated for 24 hrs by luminescent analysis	[PMID: 32442850]
NCI-H1650	GI₅₀	> 40 μM Compound: Amitriptyline	Cytotoxicity against human NCI-H1650 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26372073]
SH-SY5Y	IC₅₀	48.1 μM Compound: Amitriptyline	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability by measuring ATP content incubated for 24 hrs by luminescent analysis Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability by measuring ATP content incubated for 24 hrs by luminescent analysis	[PMID: 32442850]
Ventricular myocyte	IC₅₀	23.2 μM Compound: Amitriptyline	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]
Ventricular myocyte	IC₅₀	3.75 μM Compound: Amitriptyline	Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes	[PMID: 22761000]

Molecular Weight

277.40

Formula

C₂₀H₂₃N

CAS No.

50-48-6

Appearance

Liquid (Density: 1.076±0.06 g/cm³)

Color

Colorless to light yellow

SMILES

CN(CC/C=C1C2=CC=CC=C2CCC3=C\1C=CC=C3)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (360.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6049 mL	18.0245 mL	36.0490 mL
5 mM	0.7210 mL	3.6049 mL	7.2098 mL
10 mM	0.3605 mL	1.8025 mL	3.6049 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (255 KB) HNMR (269 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Brueckle MS, et al. Amitriptyline's anticholinergic adverse drug reactions-A systematic multiple-indication review and meta-analysis. PLoS One. 2023 Apr 5;18(4):e0284168. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6049 mL	18.0245 mL	36.0490 mL	90.1226 mL
	5 mM	0.7210 mL	3.6049 mL	7.2098 mL	18.0245 mL
	10 mM	0.3605 mL	1.8025 mL	3.6049 mL	9.0123 mL
	15 mM	0.2403 mL	1.2016 mL	2.4033 mL	6.0082 mL
	20 mM	0.1802 mL	0.9012 mL	1.8025 mL	4.5061 mL
	25 mM	0.1442 mL	0.7210 mL	1.4420 mL	3.6049 mL
	30 mM	0.1202 mL	0.6008 mL	1.2016 mL	3.0041 mL
	40 mM	0.0901 mL	0.4506 mL	0.9012 mL	2.2531 mL
	50 mM	0.0721 mL	0.3605 mL	0.7210 mL	1.8025 mL
	60 mM	0.0601 mL	0.3004 mL	0.6008 mL	1.5020 mL
	80 mM	0.0451 mL	0.2253 mL	0.4506 mL	1.1265 mL
	100 mM	0.0360 mL	0.1802 mL	0.3605 mL	0.9012 mL

Amitriptyline Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amitriptyline50-48-6Othersanticholinergic propertiesadverse drug reactionsInhibitorinhibitorinhibit

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Amitriptyline

Customer Review

Other Forms of Amitriptyline:

Amitriptyline Related Antibodies

4 Publications Citing Use of MCE Amitriptyline

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Complete Stock Solution Preparation Table

Amitriptyline Related Classifications

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