2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. Dihydrolipoic Acid

Dihydrolipoic Acid  (Synonyms: DHLA)

Cat. No.: HY-116807 Purity: 98.88%
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Dihydrolipoic Acid (DHLA) is an excellent antioxidant capable of scavenging almost any oxygen-centered radical. Dihydrolipoic acid exhibits anti-inflammatory properties in various diseases. Dihydrolipoic Acid exerts a preventive effect via ERK/Nrf2/HO-1/ROS/NLRP3 pathway in LPS-induced sickness behavior rats. Dihydrolipoic Acid can be used for the reaserch of depression.

For research use only. We do not sell to patients.

Dihydrolipoic Acid Chemical Structure

Dihydrolipoic Acid Chemical Structure

CAS No. : 462-20-4

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Based on 1 publication(s) in Google Scholar

Other Forms of Dihydrolipoic Acid:

Dihydrolipoic Acid Related Antibodies

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Description

Dihydrolipoic Acid (DHLA) is an excellent antioxidant capable of scavenging almost any oxygen-centered radical[1]. Dihydrolipoic acid exhibits anti-inflammatory properties in various diseases. Dihydrolipoic Acid exerts a preventive effect via ERK/Nrf2/HO-1/ROS/NLRP3 pathway in LPS-induced sickness behavior rats. Dihydrolipoic Acid can be used for the reaserch of depression[2].

In Vitro

Dihydrolipoic Acid is an antioxidant. DHLA is capable of scavenging ?OH radicals and scavenging superoxide radical anions with a rate constant of 3.3×105 M-1 s-1. Dihydrolipoic Acid is an excellent antioxidant, and that is very important because O2?- is a relatively mild oxidant that is therefore much more selective and can be useful to discriminate between the antioxidant capacities of different substrates[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dihydrolipoic Acid Related Antibodies
In Vivo

Dihydrolipoic Acid (DHLA) treatment exerts preventive effects in LPS-induced sickness behavior rats. Dihydrolipoic Acid increases the expression of ERK, Nrf2, and HO-1 but decreases the ROS generation levels and reduces the expression of NLRP3, caspase-1, and IL-1β in LPS-induced sickness behavior rats. Dihydrolipoic Acid is a reduced form of α-lipoic acid (LA) that can decrease oxidative stress and act as a strong antioxidant[2]. Dihydrolipoic Acid treatment reverses the LPS-induced sickness behavior[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male Sprague-Dawley (SD) rats (weight, 200-220 g)[2]
Dosage: 15 mg/kg, 30 mg/kg, 60 mg/kg
Administration: Injected intraperitoneally daily
Result: Treatment with 30 mg/kg and 60 mg/kg improved the body weight gain as compared to the LPS group.
Molecular Weight

208.34

Formula

C8H16O2S2
CAS No.
Appearance

Liquid (Density: 1.132 g/cm3)

Color

Colorless to light yellow

SMILES

OC(CCCCC(CCS)S)=O
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (479.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7998 mL 23.9992 mL 47.9985 mL
5 mM 0.9600 mL 4.7998 mL 9.5997 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.83 mg/mL (3.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.83 mg/mL (3.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.57%

SDS (698 KB)

COA (241 KB) HNMR (274 KB) RP-HPLC (218 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.7998 mL 23.9992 mL 47.9985 mL 119.9962 mL
5 mM 0.9600 mL 4.7998 mL 9.5997 mL 23.9992 mL
10 mM 0.4800 mL 2.3999 mL 4.7998 mL 11.9996 mL
15 mM 0.3200 mL 1.5999 mL 3.1999 mL 7.9997 mL
20 mM 0.2400 mL 1.2000 mL 2.3999 mL 5.9998 mL
25 mM 0.1920 mL 0.9600 mL 1.9199 mL 4.7998 mL
30 mM 0.1600 mL 0.8000 mL 1.5999 mL 3.9999 mL
40 mM 0.1200 mL 0.6000 mL 1.2000 mL 2.9999 mL
50 mM 0.0960 mL 0.4800 mL 0.9600 mL 2.3999 mL
60 mM 0.0800 mL 0.4000 mL 0.8000 mL 1.9999 mL
80 mM 0.0600 mL 0.3000 mL 0.6000 mL 1.5000 mL
100 mM 0.0480 mL 0.2400 mL 0.4800 mL 1.2000 mL
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Dihydrolipoic Acid Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dihydrolipoic Acid462-20-4DHLAReactive Oxygen SpeciesNeuroinflammationNLRP3LipopolysaccharideDepressionAntioxidantoxygencenteredradicalanti-inflammatoryERKNrf2HO-1ROSdepressionInhibitorinhibitorinhibit

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