2. Anti-infection Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. Parasite Apoptosis Reactive Oxygen Species Keap1-Nrf2 NF-κB
  4. Estragole

Estragole  (Synonyms: 4-Allylanisole)

Cat. No.: HY-N5060 Purity: 98.58%
SDS
COA
Handling Instructions Technical Support

Estragole (4-Allylanisole) is a relatively nontoxic volatile terpenoid ether and major component of the essential oil from many plants. Estragole significantly triggers Apoptosis, suppresses LPS-induced intracellular ROS production. Estragole activats Nrf-2 and regulates NF-κB. Estragole has anti-toxoplasma, anti-inflammatory, anti-edema, antioxidant and immunomodulatory properties. Estragole blocks DRG neuron excitability. Estragole has improves gastric ulcer activity.

For research use only. We do not sell to patients.

Estragole Chemical Structure

Estragole Chemical Structure

CAS No. : 140-67-0

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Liquid
500 mg In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Estragole:

Estragole Related Antibodies

Top Publications Citing Use of Products

View All Parasite Isoform Specific Products:

View All Isoforms
Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Estragole (4-Allylanisole) is a relatively nontoxic volatile terpenoid ether and major component of the essential oil from many plants. Estragole significantly triggers Apoptosis, suppresses LPS-induced intracellular ROS production. Estragole activats Nrf-2 and regulates NF-κB. Estragole has anti-toxoplasma, anti-inflammatory, anti-edema, antioxidant and immunomodulatory properties. Estragole blocks DRG neuron excitability. Estragole has improves gastric ulcer activity[1][2][3][4][5][6][7][8][9][10][11][12][13].

IC50 & Target

Toxoplasma

 

In Vitro

Estragole (0-200 μg/mL, 24 h) exhibits a dose-dependent cytotoxic activity in the monolayer culture of MCF7 cell line with IC50 value of 74 μg/mL[3].
Estragole (2000 μM, 24 h) induces apoptosis in AA8 and EM9 cells[4].
Estragole (84.5-674 μM, 2 h) exhibits anti-inflammatory activity with the regulation of NF-κB and activation of Nrf-2 signaling pathways in LPS-induced RAW 264.7 cells[5].
Estragole (1-300 μM) increases micronuclei counts (3.2-7.1 fold) in HepG2-CYP1A2 cells[6].
Estragole (31.25-500  μg/mL, 24 h) does not exhibit significant cytotoxic effects in the A. Salina and hemolysis tests[7].
Estragole (3-60 μg/mL, 30  min) inhibits neutrophil migration toward fMLP[8].
Estragole (0.6-14 mM) blocks DRG neuronal excitability by direct inhibition of Na+ channels[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Estragole Related Antibodies

Apoptosis Analysis[3]

Cell Line: MCF7
Concentration: 25, 37, 50, 74, 75, 100 μg/mL
Incubation Time: 4 h
Result: Showed apparent apoptotic morphological changes such as nuclear fragmentation, chromatin condensation, and destruction of cell membrane integrity.
Triggered apoptosis.
Showed the early apoptotic cells population of 43% after treatment with IC50/2 (IC50: 74 μg/mL) concentration for 4 h.
Increased and reached 55% after treatment with IC50 (74 μg/mL) concentration for 4 h.
Increased the activity of caspase-3.
In Vivo

Estragole (250-750 mg/kg, p.o., 30 min before the intraperitoneal injection carrageenan solution) significantly reduces the number of rolling, adherent leukocytes and decreases number of leukocyte migrated to perivascular tissue in mice[8].
Estragole (30-60 mg/kg, p.o.) shows anti-inflammatory and antiedematogenic activity, with reducing paw edema in mice[10].
Estragole (31.2-250  mg/kg, p.o., pre-treated 30  min- 1 h) prevents gastric ulcers via cytoprotective, antioxidant and immunoregulatory mechanisms in Swiss mice[11].
Estragole (100 mg/kg, p.o., daily, 6 consecutive days) shows anti-toxoplasma activity in murine models of congenital and noncongenital toxoplasmosis[12].
Estragole (100 g, i.p., once a week for 17 weeks) decreases the HNF4 and FOXA DNA-binding activities and inhibits the induction of tyrosine aminotransferase and tryptophan oxygenase only in susceptible female mice[13].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Carrageenan-induced peritonitis model[8]
Dosage: 250, 500, 750 mg/kg
Administration: Oral gavage (p.o.), 30 min before the intraperitoneal injection carrageenan solution
Result: Inhibited the leukocyte migration at doses of 500 (51%) and 750 mg/kg (52%).
Had a similar effect on anethole.
Molecular Weight

148.20

Formula

C10H12O
CAS No.
Appearance

Liquid (Density: 0.965  g/cm3  )

Color

Colorless to light yellow

SMILES

C=CCC1=CC=C(OC)C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (674.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.7476 mL 33.7382 mL 67.4764 mL
5 mM 1.3495 mL 6.7476 mL 13.4953 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.7476 mL 33.7382 mL 67.4764 mL 168.6910 mL
5 mM 1.3495 mL 6.7476 mL 13.4953 mL 33.7382 mL
10 mM 0.6748 mL 3.3738 mL 6.7476 mL 16.8691 mL
15 mM 0.4498 mL 2.2492 mL 4.4984 mL 11.2461 mL
20 mM 0.3374 mL 1.6869 mL 3.3738 mL 8.4345 mL
25 mM 0.2699 mL 1.3495 mL 2.6991 mL 6.7476 mL
30 mM 0.2249 mL 1.1246 mL 2.2492 mL 5.6230 mL
40 mM 0.1687 mL 0.8435 mL 1.6869 mL 4.2173 mL
50 mM 0.1350 mL 0.6748 mL 1.3495 mL 3.3738 mL
60 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8115 mL
80 mM 0.0843 mL 0.4217 mL 0.8435 mL 2.1086 mL
100 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6869 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Estragole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Estragole140-67-04-AllylanisoleParasiteApoptosisReactive Oxygen SpeciesKeap1-Nrf2NF-κBNuclear factor-κBNuclear factor-kappaBanti-inflammatoryanti-edemaantioxidantimmunomodulatoryMCF7RAW 264.7Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Estragole
Cat. No.:
HY-N5060
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (537 KB) Français - FR (537 KB) Deutsch - DE (537 KB) Norwegian - NO (537 KB) Español - ES (537 KB) Swedish - SV (537 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.